Lantibiotic production is a burden for the producing staphylococci

Abstract Lantibiotics are antimicrobial peptides that contain non-proteinogenic amino acids lanthionine and 3-methyllanthionine and are produced by Gram-positive bacteria. Here we addressed the pros and cons of lantibiotic production for its producing strains. Two staphylococcal strains, S. gallinar...

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Autores principales:	Patrick Ebner, Sebastian Reichert, Arif Luqman, Bernhard Krismer, Peter Popella, Friedrich Götz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a412eab7b0dd498fbb338e53ac075f5f
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spelling	oai:doaj.org-article:a412eab7b0dd498fbb338e53ac075f5f2021-12-02T16:08:02ZLantibiotic production is a burden for the producing staphylococci10.1038/s41598-018-25935-22045-2322https://doaj.org/article/a412eab7b0dd498fbb338e53ac075f5f2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25935-2https://doaj.org/toc/2045-2322Abstract Lantibiotics are antimicrobial peptides that contain non-proteinogenic amino acids lanthionine and 3-methyllanthionine and are produced by Gram-positive bacteria. Here we addressed the pros and cons of lantibiotic production for its producing strains. Two staphylococcal strains, S. gallinarum Tü3928 and S. epidermidis Tü3298 producing gallidermin and epidermin respectively were selected. In each of these parental strains, the structural genes gdmA and epiA were deleted; all the other biosynthetic genes including the immunity genes were left intact. Comparative analysis of the lantibiotic-producing strains with their non-producing mutants revealed that lantibiotic production is a burden for the cells. The production affected growth, caused release of ATP, lipids and increased the excretion of cytoplasmic proteins (ECP). The epidermin and gallidermin immunity genes were insufficient to protect the cells from their own product. Co-cultivation studies showed that the ΔgdmA mutant has an advantage over the parental strain; the latter was outcompeted. On the one hand, the production of staphylococcal lantibiotics is beneficial by suppressing competitors, but on the other hand they impose a burden on the producing-strains when they accumulate in higher amounts. Our observations explain why antibiotic-producing strains occur as a minority on our skin and other ecological niches, but retain corresponding antibiotic resistance.Patrick EbnerSebastian ReichertArif LuqmanBernhard KrismerPeter PopellaFriedrich GötzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Patrick Ebner Sebastian Reichert Arif Luqman Bernhard Krismer Peter Popella Friedrich Götz Lantibiotic production is a burden for the producing staphylococci
description	Abstract Lantibiotics are antimicrobial peptides that contain non-proteinogenic amino acids lanthionine and 3-methyllanthionine and are produced by Gram-positive bacteria. Here we addressed the pros and cons of lantibiotic production for its producing strains. Two staphylococcal strains, S. gallinarum Tü3928 and S. epidermidis Tü3298 producing gallidermin and epidermin respectively were selected. In each of these parental strains, the structural genes gdmA and epiA were deleted; all the other biosynthetic genes including the immunity genes were left intact. Comparative analysis of the lantibiotic-producing strains with their non-producing mutants revealed that lantibiotic production is a burden for the cells. The production affected growth, caused release of ATP, lipids and increased the excretion of cytoplasmic proteins (ECP). The epidermin and gallidermin immunity genes were insufficient to protect the cells from their own product. Co-cultivation studies showed that the ΔgdmA mutant has an advantage over the parental strain; the latter was outcompeted. On the one hand, the production of staphylococcal lantibiotics is beneficial by suppressing competitors, but on the other hand they impose a burden on the producing-strains when they accumulate in higher amounts. Our observations explain why antibiotic-producing strains occur as a minority on our skin and other ecological niches, but retain corresponding antibiotic resistance.
format	article
author	Patrick Ebner Sebastian Reichert Arif Luqman Bernhard Krismer Peter Popella Friedrich Götz
author_facet	Patrick Ebner Sebastian Reichert Arif Luqman Bernhard Krismer Peter Popella Friedrich Götz
author_sort	Patrick Ebner
title	Lantibiotic production is a burden for the producing staphylococci
title_short	Lantibiotic production is a burden for the producing staphylococci
title_full	Lantibiotic production is a burden for the producing staphylococci
title_fullStr	Lantibiotic production is a burden for the producing staphylococci
title_full_unstemmed	Lantibiotic production is a burden for the producing staphylococci
title_sort	lantibiotic production is a burden for the producing staphylococci
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a412eab7b0dd498fbb338e53ac075f5f
work_keys_str_mv	AT patrickebner lantibioticproductionisaburdenfortheproducingstaphylococci AT sebastianreichert lantibioticproductionisaburdenfortheproducingstaphylococci AT arifluqman lantibioticproductionisaburdenfortheproducingstaphylococci AT bernhardkrismer lantibioticproductionisaburdenfortheproducingstaphylococci AT peterpopella lantibioticproductionisaburdenfortheproducingstaphylococci AT friedrichgotz lantibioticproductionisaburdenfortheproducingstaphylococci
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Lantibiotic production is a burden for the producing staphylococci

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