Ex-Vivo Adhesion of <i>Enterococcus faecalis</i> and <i>Enterococcus faecium</i> to the Intestinal Mucosa of Healthy Beagles
Some <i>Enterococcus faecalis</i> and <i>E. faecium</i> strains are used as probiotics or feed additives. Adherence to the intestinal mucosa is considered a crucial step for intestinal bacteria to colonize and further interact with the host epithelium and the immune system. I...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/a415aa587a064f258bafca7476045232 |
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| Sumario: | Some <i>Enterococcus faecalis</i> and <i>E. faecium</i> strains are used as probiotics or feed additives. Adherence to the intestinal mucosa is considered a crucial step for intestinal bacteria to colonize and further interact with the host epithelium and the immune system. In dogs, there are no studies investigating the adhesion of <i>E. faecalis</i> and <i>E. faecium</i> to paraffin-embedded intestinal mucosa. Therefore, we aimed to investigate the adhesion of <i>E. faecalis</i> and <i>E. faecium</i> to the intestinal mucosa of six healthy beagles using bacteria derived from dogs and chickens. In addition, we aimed to validate a method to test the adhesion of Alexa Fluor-labeled bacteria to paraffin-embedded canine intestinal mucosa. The results of our study show that both canine- and chicken-derived <i>E. faecalis</i> strains adhered significantly better than <i>E. faecium</i> to the duodenal mucosa of healthy beagles (<i>p</i> = 0.002). In addition, canine <i>E. faecalis</i> and <i>E. faecium</i> adhered in higher numbers to canine duodenal mucosa, compared to chicken-derived strains of the same species (<i>p</i> = 0.015 for <i>E. faecalis</i> and <i>p</i> = 0.002 for <i>E. faecium</i>). The determination of the hydrophobicity of bacteria revealed that canine <i>E. faecalis</i> had the highest hydrophobicity level (36.6%), followed by chicken <i>E. faecalis</i> (20.4%), while canine <i>E. faecium</i> (5.7%) and chicken <i>E. faecium</i> (4.5%) had the lowest levels. Our results suggest that both the bacterial species and the host origin of the strain may influence mucosal adhesion. |
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