Microsatellite polymorphism for molecular characterization of pomelo (Citrus maxima) accessions from Indonesia

Abstract. Susandarini R, Subandiyah S, Daryono BS, Rugayah. 2020. Microsatellite polymorphism for molecular characterization of pomelo (Citrus maxima) accessions from Indonesia. Biodiversitas 21: 2390-2395. Citrus maxima (Burm.) Merr. (pomelo) as a minor fruit crop deserve attention on its phenotypi...

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Autores principales:	Ratna Susandarini, Siti Subandiyah, Budi Setiadi Daryono, Rugayah
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	citrus maxima, microsatellite, characterization, genotypic variability Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a41646f468f84069848d8eb709feeb6e
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spelling	oai:doaj.org-article:a41646f468f84069848d8eb709feeb6e2021-11-22T00:40:46ZMicrosatellite polymorphism for molecular characterization of pomelo (Citrus maxima) accessions from Indonesia1412-033X2085-472210.13057/biodiv/d210608https://doaj.org/article/a41646f468f84069848d8eb709feeb6e2020-05-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5774https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Susandarini R, Subandiyah S, Daryono BS, Rugayah. 2020. Microsatellite polymorphism for molecular characterization of pomelo (Citrus maxima) accessions from Indonesia. Biodiversitas 21: 2390-2395. Citrus maxima (Burm.) Merr. (pomelo) as a minor fruit crop deserve attention on its phenotypic and genotypic variability to avoid the risk of extinction. Previous study showed that pomelo from Indonesia has high morphological variability, and thus it is interesting to explore its genotypic variability using molecular markers. Microsatellite is a molecular marker widely used in Citrus taxonomy studies. This study aimed at revealing microsatellite polymorphism and its potential application in cultivar characterization of C .maxima. Eighty accessions of C. maxima consisting of registered cultivars and landraces from Indonesia were used in this study. Analysis of microsatellite sequences from genomic DNA amplified using DY296883 primer showed that C. maxima microsatellite has high polymorphism in the form of repeat length variation of (GA)n, ranging from (GA)7 to (GA)19. This study proved the existence of high genotypic variability in C. maxima, and confirmed the role of microsatellite as a useful molecular marker for uncovering variability at intraspecific level. Observation of the microsatellite polymorphisms indicated that variability of (GA)n can be used to distinguish some pomelo cultivars.Ratna SusandariniSiti SubandiyahBudi Setiadi DaryonoRugayahMBI & UNS Soloarticlecitrus maxima, microsatellite, characterization, genotypic variabilityBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 6 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	citrus maxima, microsatellite, characterization, genotypic variability Biology (General) QH301-705.5
spellingShingle	citrus maxima, microsatellite, characterization, genotypic variability Biology (General) QH301-705.5 Ratna Susandarini Siti Subandiyah Budi Setiadi Daryono Rugayah Microsatellite polymorphism for molecular characterization of pomelo (Citrus maxima) accessions from Indonesia
description	Abstract. Susandarini R, Subandiyah S, Daryono BS, Rugayah. 2020. Microsatellite polymorphism for molecular characterization of pomelo (Citrus maxima) accessions from Indonesia. Biodiversitas 21: 2390-2395. Citrus maxima (Burm.) Merr. (pomelo) as a minor fruit crop deserve attention on its phenotypic and genotypic variability to avoid the risk of extinction. Previous study showed that pomelo from Indonesia has high morphological variability, and thus it is interesting to explore its genotypic variability using molecular markers. Microsatellite is a molecular marker widely used in Citrus taxonomy studies. This study aimed at revealing microsatellite polymorphism and its potential application in cultivar characterization of C .maxima. Eighty accessions of C. maxima consisting of registered cultivars and landraces from Indonesia were used in this study. Analysis of microsatellite sequences from genomic DNA amplified using DY296883 primer showed that C. maxima microsatellite has high polymorphism in the form of repeat length variation of (GA)n, ranging from (GA)7 to (GA)19. This study proved the existence of high genotypic variability in C. maxima, and confirmed the role of microsatellite as a useful molecular marker for uncovering variability at intraspecific level. Observation of the microsatellite polymorphisms indicated that variability of (GA)n can be used to distinguish some pomelo cultivars.
format	article
author	Ratna Susandarini Siti Subandiyah Budi Setiadi Daryono Rugayah
author_facet	Ratna Susandarini Siti Subandiyah Budi Setiadi Daryono Rugayah
author_sort	Ratna Susandarini
title	Microsatellite polymorphism for molecular characterization of pomelo (Citrus maxima) accessions from Indonesia
title_short	Microsatellite polymorphism for molecular characterization of pomelo (Citrus maxima) accessions from Indonesia
title_full	Microsatellite polymorphism for molecular characterization of pomelo (Citrus maxima) accessions from Indonesia
title_fullStr	Microsatellite polymorphism for molecular characterization of pomelo (Citrus maxima) accessions from Indonesia
title_full_unstemmed	Microsatellite polymorphism for molecular characterization of pomelo (Citrus maxima) accessions from Indonesia
title_sort	microsatellite polymorphism for molecular characterization of pomelo (citrus maxima) accessions from indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/a41646f468f84069848d8eb709feeb6e
work_keys_str_mv	AT ratnasusandarini microsatellitepolymorphismformolecularcharacterizationofpomelocitrusmaximaaccessionsfromindonesia AT sitisubandiyah microsatellitepolymorphismformolecularcharacterizationofpomelocitrusmaximaaccessionsfromindonesia AT budisetiadidaryono microsatellitepolymorphismformolecularcharacterizationofpomelocitrusmaximaaccessionsfromindonesia AT rugayah microsatellitepolymorphismformolecularcharacterizationofpomelocitrusmaximaaccessionsfromindonesia
_version_	1718418542021312512

Microsatellite polymorphism for molecular characterization of pomelo (Citrus maxima) accessions from Indonesia

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