Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia

Cucurbits are important crops in the world. However, leaf curl disease constrains their production. Here, begomovirus diversity and pathogenicity associated with the disease in Malaysia were studied based on 49 begomovirus-detected out of 69 symptomatic plants from seven cucurbit crops in 15 locatio...

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Autores principales:	Yu-Jeng Chen, Hsuan-Chun Lai, Chung-Cheng Lin, Zhuan Yi Neoh, Wen-Shi Tsai
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Squash leaf curl China virus</i> <i>Tomato leaf curl New Delhi virus</i> agroinoculation polymerase chain reaction host range Botany QK1-989
Acceso en línea:	https://doaj.org/article/a418bbef67b74e79b09479a67dae184d
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spelling	oai:doaj.org-article:a418bbef67b74e79b09479a67dae184d2021-11-25T18:46:16ZGenetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia10.3390/plants101123962223-7747https://doaj.org/article/a418bbef67b74e79b09479a67dae184d2021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2396https://doaj.org/toc/2223-7747Cucurbits are important crops in the world. However, leaf curl disease constrains their production. Here, begomovirus diversity and pathogenicity associated with the disease in Malaysia were studied based on 49 begomovirus-detected out of 69 symptomatic plants from seven cucurbit crops in 15 locations during 2016 and 2017. The presence of <i>Squash leaf curl China virus</i> (SLCCNV) and <i>Tomato leaf curl New Delhi virus</i> (ToLCNDV) were confirmed by virus detection by polymerase chain reaction, viral DNA sequence analysis and specific detection of the viral components. ToLCNDV Malaysian isolates were further distinguished into strains A, B, C and D. Virus co-infection was detected in bitter gourd, bottle gourd and squash. Among them, eight bitter gourd samples were detected without SLCCNV DNA-A. However, one bottle gourd and five squash samples were without ToLCNDV DNA-B. Pseudorecombination of ToLCNDV DNA-A and SLCCNV DNA-B was detected in two bitter gourd samples. The pathogenic viruses and pseudorecombinants were confirmed by agroinoculation. The viral DNA-B influencing on symptomology and host range was also confirmed. The results strengthen the epidemic of cucurbit-infecting begomovirus in Malaysia as well as Southeast Asia. Especially, the natural pseudorecombinant of begomovirus that extends host range and causes severe symptom implies a threat to crops.Yu-Jeng ChenHsuan-Chun LaiChung-Cheng LinZhuan Yi NeohWen-Shi TsaiMDPI AGarticle<i>Squash leaf curl China virus</i><i>Tomato leaf curl New Delhi virus</i>agroinoculationpolymerase chain reactionhost rangeBotanyQK1-989ENPlants, Vol 10, Iss 2396, p 2396 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Squash leaf curl China virus</i> <i>Tomato leaf curl New Delhi virus</i> agroinoculation polymerase chain reaction host range Botany QK1-989
spellingShingle	<i>Squash leaf curl China virus</i> <i>Tomato leaf curl New Delhi virus</i> agroinoculation polymerase chain reaction host range Botany QK1-989 Yu-Jeng Chen Hsuan-Chun Lai Chung-Cheng Lin Zhuan Yi Neoh Wen-Shi Tsai Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia
description	Cucurbits are important crops in the world. However, leaf curl disease constrains their production. Here, begomovirus diversity and pathogenicity associated with the disease in Malaysia were studied based on 49 begomovirus-detected out of 69 symptomatic plants from seven cucurbit crops in 15 locations during 2016 and 2017. The presence of <i>Squash leaf curl China virus</i> (SLCCNV) and <i>Tomato leaf curl New Delhi virus</i> (ToLCNDV) were confirmed by virus detection by polymerase chain reaction, viral DNA sequence analysis and specific detection of the viral components. ToLCNDV Malaysian isolates were further distinguished into strains A, B, C and D. Virus co-infection was detected in bitter gourd, bottle gourd and squash. Among them, eight bitter gourd samples were detected without SLCCNV DNA-A. However, one bottle gourd and five squash samples were without ToLCNDV DNA-B. Pseudorecombination of ToLCNDV DNA-A and SLCCNV DNA-B was detected in two bitter gourd samples. The pathogenic viruses and pseudorecombinants were confirmed by agroinoculation. The viral DNA-B influencing on symptomology and host range was also confirmed. The results strengthen the epidemic of cucurbit-infecting begomovirus in Malaysia as well as Southeast Asia. Especially, the natural pseudorecombinant of begomovirus that extends host range and causes severe symptom implies a threat to crops.
format	article
author	Yu-Jeng Chen Hsuan-Chun Lai Chung-Cheng Lin Zhuan Yi Neoh Wen-Shi Tsai
author_facet	Yu-Jeng Chen Hsuan-Chun Lai Chung-Cheng Lin Zhuan Yi Neoh Wen-Shi Tsai
author_sort	Yu-Jeng Chen
title	Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia
title_short	Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia
title_full	Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia
title_fullStr	Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia
title_full_unstemmed	Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia
title_sort	genetic diversity, pathogenicity and pseudorecombination of cucurbit-infecting begomoviruses in malaysia
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a418bbef67b74e79b09479a67dae184d
work_keys_str_mv	AT yujengchen geneticdiversitypathogenicityandpseudorecombinationofcucurbitinfectingbegomovirusesinmalaysia AT hsuanchunlai geneticdiversitypathogenicityandpseudorecombinationofcucurbitinfectingbegomovirusesinmalaysia AT chungchenglin geneticdiversitypathogenicityandpseudorecombinationofcucurbitinfectingbegomovirusesinmalaysia AT zhuanyineoh geneticdiversitypathogenicityandpseudorecombinationofcucurbitinfectingbegomovirusesinmalaysia AT wenshitsai geneticdiversitypathogenicityandpseudorecombinationofcucurbitinfectingbegomovirusesinmalaysia
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Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia

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