Expression Profile and Ligand Screening of a Putative Odorant-Binding Protein, AcerOBP6, from the Asian Honeybee

Olfaction is essential in some behaviors of honeybee, such as nursing, foraging, attracting a mate, social communication, and kin recognition. OBPs (odorant binding proteins) play a key role in the first step of olfactory perception. Here, we focused on a classic OBP with a PBP-GOBP domain from the...

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Autores principales:	Huiting Zhao, Zhu Peng, Li Huang, Shuguo Zhao, Miaomiao Liu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	honeybee odorant binding protein fluorescence binding assay EAG Science Q
Acceso en línea:	https://doaj.org/article/a41b0e3c93314c9b883e4ee2247414c8
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spelling	oai:doaj.org-article:a41b0e3c93314c9b883e4ee2247414c82021-11-25T17:59:02ZExpression Profile and Ligand Screening of a Putative Odorant-Binding Protein, AcerOBP6, from the Asian Honeybee10.3390/insects121109552075-4450https://doaj.org/article/a41b0e3c93314c9b883e4ee2247414c82021-10-01T00:00:00Zhttps://www.mdpi.com/2075-4450/12/11/955https://doaj.org/toc/2075-4450Olfaction is essential in some behaviors of honeybee, such as nursing, foraging, attracting a mate, social communication, and kin recognition. OBPs (odorant binding proteins) play a key role in the first step of olfactory perception. Here, we focused on a classic OBP with a PBP-GOBP domain from the Asian honeybee, <i>Apis cerana cerana</i>. Beyond that, the mRNA expression profiles and the binding affinity of AcerOBP6 were researched. According to qRT-PCR analysis, AcerOBP6 transcripts were mainly expressed in the antennae of forager bees. In addition, we found that the expression level of AcerOBP6 was higher than that of AmelOBP6. The fluorescence competitive binding assay indicated that the AcerOBP6 protein had binding affinity with most of the tested odors, including queen pheromone, worker pheromone, and floral volatiles, among which the strongest one was linolenic acid (with a Ki value of 1.67). However, AcerOBP6 was not sensitive to the brood pheromones. A further study based on EAG assay revealed that the antennae had the strongest response to 2-heptanone. The EAG recording values of the selected ligands were all reduced after AcerOBP6 was silenced, with 8 of 14 declining significantly (<i>p</i> < 0.01) given that these odors could specifically bind to AcerOBP6. As revealed in our current study, AcerOBP6 might be a crucial protein involved in olfactory recognition for foraging. Overall, the research provides a foundation for exploring the olfactory mechanism of <i>A. cerana cerana</i>.Huiting ZhaoZhu PengLi HuangShuguo ZhaoMiaomiao LiuMDPI AGarticlehoneybeeodorant binding proteinfluorescence binding assayEAGScienceQENInsects, Vol 12, Iss 955, p 955 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	honeybee odorant binding protein fluorescence binding assay EAG Science Q
spellingShingle	honeybee odorant binding protein fluorescence binding assay EAG Science Q Huiting Zhao Zhu Peng Li Huang Shuguo Zhao Miaomiao Liu Expression Profile and Ligand Screening of a Putative Odorant-Binding Protein, AcerOBP6, from the Asian Honeybee
description	Olfaction is essential in some behaviors of honeybee, such as nursing, foraging, attracting a mate, social communication, and kin recognition. OBPs (odorant binding proteins) play a key role in the first step of olfactory perception. Here, we focused on a classic OBP with a PBP-GOBP domain from the Asian honeybee, <i>Apis cerana cerana</i>. Beyond that, the mRNA expression profiles and the binding affinity of AcerOBP6 were researched. According to qRT-PCR analysis, AcerOBP6 transcripts were mainly expressed in the antennae of forager bees. In addition, we found that the expression level of AcerOBP6 was higher than that of AmelOBP6. The fluorescence competitive binding assay indicated that the AcerOBP6 protein had binding affinity with most of the tested odors, including queen pheromone, worker pheromone, and floral volatiles, among which the strongest one was linolenic acid (with a Ki value of 1.67). However, AcerOBP6 was not sensitive to the brood pheromones. A further study based on EAG assay revealed that the antennae had the strongest response to 2-heptanone. The EAG recording values of the selected ligands were all reduced after AcerOBP6 was silenced, with 8 of 14 declining significantly (<i>p</i> < 0.01) given that these odors could specifically bind to AcerOBP6. As revealed in our current study, AcerOBP6 might be a crucial protein involved in olfactory recognition for foraging. Overall, the research provides a foundation for exploring the olfactory mechanism of <i>A. cerana cerana</i>.
format	article
author	Huiting Zhao Zhu Peng Li Huang Shuguo Zhao Miaomiao Liu
author_facet	Huiting Zhao Zhu Peng Li Huang Shuguo Zhao Miaomiao Liu
author_sort	Huiting Zhao
title	Expression Profile and Ligand Screening of a Putative Odorant-Binding Protein, AcerOBP6, from the Asian Honeybee
title_short	Expression Profile and Ligand Screening of a Putative Odorant-Binding Protein, AcerOBP6, from the Asian Honeybee
title_full	Expression Profile and Ligand Screening of a Putative Odorant-Binding Protein, AcerOBP6, from the Asian Honeybee
title_fullStr	Expression Profile and Ligand Screening of a Putative Odorant-Binding Protein, AcerOBP6, from the Asian Honeybee
title_full_unstemmed	Expression Profile and Ligand Screening of a Putative Odorant-Binding Protein, AcerOBP6, from the Asian Honeybee
title_sort	expression profile and ligand screening of a putative odorant-binding protein, acerobp6, from the asian honeybee
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a41b0e3c93314c9b883e4ee2247414c8
work_keys_str_mv	AT huitingzhao expressionprofileandligandscreeningofaputativeodorantbindingproteinacerobp6fromtheasianhoneybee AT zhupeng expressionprofileandligandscreeningofaputativeodorantbindingproteinacerobp6fromtheasianhoneybee AT lihuang expressionprofileandligandscreeningofaputativeodorantbindingproteinacerobp6fromtheasianhoneybee AT shuguozhao expressionprofileandligandscreeningofaputativeodorantbindingproteinacerobp6fromtheasianhoneybee AT miaomiaoliu expressionprofileandligandscreeningofaputativeodorantbindingproteinacerobp6fromtheasianhoneybee
_version_	1718411764434993152

Expression Profile and Ligand Screening of a Putative Odorant-Binding Protein, AcerOBP6, from the Asian Honeybee

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