Effects of miR-185-5p on replication of hepatitis C virus

This article was designed to explore the effects and mechanisms of miR-185-5p on the replication of hepatitis C virus (HCV). Quantitative reverse transcription PCR (qRT-PCR) was performed for detecting the abundance of miR-185-5p and HCV RNA in HCV-infected primary hepatocytes and Huh7.5 cells. Dual...

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Autores principales:	Huang Wei, Song Lingyan, Zhang Jingyan, Yan Xueqiang, Yan Hui
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	hepatitis c virus replication mir-185-5p galnt8 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a41b464e3a6846bf95243c4235653eda
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spelling	oai:doaj.org-article:a41b464e3a6846bf95243c4235653eda2021-12-05T14:10:41ZEffects of miR-185-5p on replication of hepatitis C virus2391-541210.1515/biol-2021-0067https://doaj.org/article/a41b464e3a6846bf95243c4235653eda2021-08-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0067https://doaj.org/toc/2391-5412This article was designed to explore the effects and mechanisms of miR-185-5p on the replication of hepatitis C virus (HCV). Quantitative reverse transcription PCR (qRT-PCR) was performed for detecting the abundance of miR-185-5p and HCV RNA in HCV-infected primary hepatocytes and Huh7.5 cells. Dual-luciferase reporter gene assay was used for exploring the interaction between miR-185-5p and GALNT8. Western blot analyzed protein expression of GALNT8, NS3, and NS5A. miR-185-5p was remarkably downregulated in HCV-infected primary hepatocytes and Huh7.5 cells. miR-185-5p upregulation inhibited HCV RNA expression, while its inhibition promoted HCV replication. miR-185-5p induced accumulation of NS3 and NS5A in the cells. Dual-luciferase reporter gene assay verified the targeted relationship between miR-185-5p and GALNT8. In addition, the effects of overexpressing or knocking down miR-185-5p on HCV replication could be correspondingly eliminated by the overexpression or knockdown of GALNT8. miR-185-5p may target GALNT8 in JFH1-infected Huh7.5 cells and then inhibit HCV replication. miR-185-5p may be a potential target for treating HCV.Huang WeiSong LingyanZhang JingyanYan XueqiangYan HuiDe Gruyterarticlehepatitis c virusreplicationmir-185-5pgalnt8Biology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 752-757 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hepatitis c virus replication mir-185-5p galnt8 Biology (General) QH301-705.5
spellingShingle	hepatitis c virus replication mir-185-5p galnt8 Biology (General) QH301-705.5 Huang Wei Song Lingyan Zhang Jingyan Yan Xueqiang Yan Hui Effects of miR-185-5p on replication of hepatitis C virus
description	This article was designed to explore the effects and mechanisms of miR-185-5p on the replication of hepatitis C virus (HCV). Quantitative reverse transcription PCR (qRT-PCR) was performed for detecting the abundance of miR-185-5p and HCV RNA in HCV-infected primary hepatocytes and Huh7.5 cells. Dual-luciferase reporter gene assay was used for exploring the interaction between miR-185-5p and GALNT8. Western blot analyzed protein expression of GALNT8, NS3, and NS5A. miR-185-5p was remarkably downregulated in HCV-infected primary hepatocytes and Huh7.5 cells. miR-185-5p upregulation inhibited HCV RNA expression, while its inhibition promoted HCV replication. miR-185-5p induced accumulation of NS3 and NS5A in the cells. Dual-luciferase reporter gene assay verified the targeted relationship between miR-185-5p and GALNT8. In addition, the effects of overexpressing or knocking down miR-185-5p on HCV replication could be correspondingly eliminated by the overexpression or knockdown of GALNT8. miR-185-5p may target GALNT8 in JFH1-infected Huh7.5 cells and then inhibit HCV replication. miR-185-5p may be a potential target for treating HCV.
format	article
author	Huang Wei Song Lingyan Zhang Jingyan Yan Xueqiang Yan Hui
author_facet	Huang Wei Song Lingyan Zhang Jingyan Yan Xueqiang Yan Hui
author_sort	Huang Wei
title	Effects of miR-185-5p on replication of hepatitis C virus
title_short	Effects of miR-185-5p on replication of hepatitis C virus
title_full	Effects of miR-185-5p on replication of hepatitis C virus
title_fullStr	Effects of miR-185-5p on replication of hepatitis C virus
title_full_unstemmed	Effects of miR-185-5p on replication of hepatitis C virus
title_sort	effects of mir-185-5p on replication of hepatitis c virus
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/a41b464e3a6846bf95243c4235653eda
work_keys_str_mv	AT huangwei effectsofmir1855ponreplicationofhepatitiscvirus AT songlingyan effectsofmir1855ponreplicationofhepatitiscvirus AT zhangjingyan effectsofmir1855ponreplicationofhepatitiscvirus AT yanxueqiang effectsofmir1855ponreplicationofhepatitiscvirus AT yanhui effectsofmir1855ponreplicationofhepatitiscvirus
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Effects of miR-185-5p on replication of hepatitis C virus

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