Genetic Diversity and Transmission of Multidrug-Resistant Mycobacterium tuberculosis strains in Lusaka, Zambia

ABSTRACT: Objective: Zambia is among the 30 high tuberculosis burden countries in the world. Despite increasing reports of multidrug-resistant tuberculosis (MDR-TB) in routine surveillance, information on the transmission of MDR Mycobacterium tuberculosis strains is largely unknown. This study eluc...

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Autores principales:	Joseph Yamweka Chizimu, Eddie Samuneti Solo, Precious Bwalya, Thoko Flav Kapalamula, Mwangala Lonah Akapelwa, Patrick Lungu, Dipti Shrestha, Yukari Fukushima, Victor Mukonka, Jeewan Thapa, Chie Nakajima, Yasuhiko Suzuki
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Mycobacterium tuberculosis multidrug resistance mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) Zambia Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/a4247faa5f1b40d0a4985862dbd022d7
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spelling	oai:doaj.org-article:a4247faa5f1b40d0a4985862dbd022d72021-12-02T04:59:43ZGenetic Diversity and Transmission of Multidrug-Resistant Mycobacterium tuberculosis strains in Lusaka, Zambia1201-971210.1016/j.ijid.2021.10.044https://doaj.org/article/a4247faa5f1b40d0a4985862dbd022d72022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1201971221008262https://doaj.org/toc/1201-9712ABSTRACT: Objective: Zambia is among the 30 high tuberculosis burden countries in the world. Despite increasing reports of multidrug-resistant tuberculosis (MDR-TB) in routine surveillance, information on the transmission of MDR Mycobacterium tuberculosis strains is largely unknown. This study elucidated the genetic diversity and transmission of MDR M. tuberculosis strains in Lusaka, Zambia. Methods: Eighty-five MDR M. tuberculosis samples collected from 2013 to 2017 at the University Teaching Hospital were used. Drug-resistance associated gene sequencing, spoligotyping, 24-loci mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR), and multiplex PCR for RD-Rio sub-lineage identification were applied. Results: The identified clades were LAM (48%), CAS (29%), T (14%), X (6%) and Harlem (2%). Strains belonging to SITs 21/CAS1-Kili and 20/LAM1 formed the largest clonal complexes. Combined spoligotyping and 24 loci-MIRU-VNTR revealed 47 genotypic patterns with a clustering rate of 63%. Ninety-five percent of LAM strains belonged to the RD-Rio sub-lineage. Conclusion: The high clustering rate suggested that a large proportion of MDR-TB was due to recent transmission rather than the independent acquisition of MDR. This spread was attributed to clonal expansion of SIT21/CAS1-Kili and SIT20/LAM1 strains. Therefore, TB control programs recommending genotyping coupled with conventional epidemiological methods can guide measures for stopping the spread of MDR-TB.Joseph Yamweka ChizimuEddie Samuneti SoloPrecious BwalyaThoko Flav KapalamulaMwangala Lonah AkapelwaPatrick LunguDipti ShresthaYukari FukushimaVictor MukonkaJeewan ThapaChie NakajimaYasuhiko SuzukiElsevierarticleMycobacterium tuberculosismultidrug resistancemycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR)ZambiaInfectious and parasitic diseasesRC109-216ENInternational Journal of Infectious Diseases, Vol 114, Iss , Pp 142-150 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mycobacterium tuberculosis multidrug resistance mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) Zambia Infectious and parasitic diseases RC109-216
spellingShingle	Mycobacterium tuberculosis multidrug resistance mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) Zambia Infectious and parasitic diseases RC109-216 Joseph Yamweka Chizimu Eddie Samuneti Solo Precious Bwalya Thoko Flav Kapalamula Mwangala Lonah Akapelwa Patrick Lungu Dipti Shrestha Yukari Fukushima Victor Mukonka Jeewan Thapa Chie Nakajima Yasuhiko Suzuki Genetic Diversity and Transmission of Multidrug-Resistant Mycobacterium tuberculosis strains in Lusaka, Zambia
description	ABSTRACT: Objective: Zambia is among the 30 high tuberculosis burden countries in the world. Despite increasing reports of multidrug-resistant tuberculosis (MDR-TB) in routine surveillance, information on the transmission of MDR Mycobacterium tuberculosis strains is largely unknown. This study elucidated the genetic diversity and transmission of MDR M. tuberculosis strains in Lusaka, Zambia. Methods: Eighty-five MDR M. tuberculosis samples collected from 2013 to 2017 at the University Teaching Hospital were used. Drug-resistance associated gene sequencing, spoligotyping, 24-loci mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR), and multiplex PCR for RD-Rio sub-lineage identification were applied. Results: The identified clades were LAM (48%), CAS (29%), T (14%), X (6%) and Harlem (2%). Strains belonging to SITs 21/CAS1-Kili and 20/LAM1 formed the largest clonal complexes. Combined spoligotyping and 24 loci-MIRU-VNTR revealed 47 genotypic patterns with a clustering rate of 63%. Ninety-five percent of LAM strains belonged to the RD-Rio sub-lineage. Conclusion: The high clustering rate suggested that a large proportion of MDR-TB was due to recent transmission rather than the independent acquisition of MDR. This spread was attributed to clonal expansion of SIT21/CAS1-Kili and SIT20/LAM1 strains. Therefore, TB control programs recommending genotyping coupled with conventional epidemiological methods can guide measures for stopping the spread of MDR-TB.
format	article
author	Joseph Yamweka Chizimu Eddie Samuneti Solo Precious Bwalya Thoko Flav Kapalamula Mwangala Lonah Akapelwa Patrick Lungu Dipti Shrestha Yukari Fukushima Victor Mukonka Jeewan Thapa Chie Nakajima Yasuhiko Suzuki
author_facet	Joseph Yamweka Chizimu Eddie Samuneti Solo Precious Bwalya Thoko Flav Kapalamula Mwangala Lonah Akapelwa Patrick Lungu Dipti Shrestha Yukari Fukushima Victor Mukonka Jeewan Thapa Chie Nakajima Yasuhiko Suzuki
author_sort	Joseph Yamweka Chizimu
title	Genetic Diversity and Transmission of Multidrug-Resistant Mycobacterium tuberculosis strains in Lusaka, Zambia
title_short	Genetic Diversity and Transmission of Multidrug-Resistant Mycobacterium tuberculosis strains in Lusaka, Zambia
title_full	Genetic Diversity and Transmission of Multidrug-Resistant Mycobacterium tuberculosis strains in Lusaka, Zambia
title_fullStr	Genetic Diversity and Transmission of Multidrug-Resistant Mycobacterium tuberculosis strains in Lusaka, Zambia
title_full_unstemmed	Genetic Diversity and Transmission of Multidrug-Resistant Mycobacterium tuberculosis strains in Lusaka, Zambia
title_sort	genetic diversity and transmission of multidrug-resistant mycobacterium tuberculosis strains in lusaka, zambia
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/a4247faa5f1b40d0a4985862dbd022d7
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Genetic Diversity and Transmission of Multidrug-Resistant Mycobacterium tuberculosis strains in Lusaka, Zambia

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