Colonization factor CS30 from enterotoxigenic Escherichia coli binds to sulfatide in human and porcine small intestine

The ability to adhere via colonization factors to specific receptors located on the intestinal mucosa is a key virulence factor in enterotoxigenic Escherichia coli (ETEC) pathogenesis. Here, the potential glycosphingolipid receptors of the novel human ETEC colonization factor CS30 were examined by b...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Astrid Von Mentzer, Dani Zalem, Zofia Chrienova, Susann Teneberg
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	enterotoxigenic e. coli colonization factor cs30 microbial adhesion carbohydrate binding glycosphingolipid characterization sulfatide Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/a428fc35df25481694a39ad273dced73
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a428fc35df25481694a39ad273dced73
record_format	dspace
spelling	oai:doaj.org-article:a428fc35df25481694a39ad273dced732021-11-17T14:21:58ZColonization factor CS30 from enterotoxigenic Escherichia coli binds to sulfatide in human and porcine small intestine2150-55942150-560810.1080/21505594.2020.1749497https://doaj.org/article/a428fc35df25481694a39ad273dced732020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1749497https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608The ability to adhere via colonization factors to specific receptors located on the intestinal mucosa is a key virulence factor in enterotoxigenic Escherichia coli (ETEC) pathogenesis. Here, the potential glycosphingolipid receptors of the novel human ETEC colonization factor CS30 were examined by binding of CS30-expressing bacteria to glycosphingolipids on thin-layer chromatograms. We thereby found a highly specific binding of CS30-expressing bacteria to a fast-migrating acid glycosphingolipid of human and porcine small intestine, while no binding was obtained with a mutant ETEC strain unable to express CS30 fimbriae. The CS30 binding glycosphingolipid from human small intestine was isolated and characterized by mass spectrometry as sulfatide (SO3-3Galβ1Cer). Comparative binding studies using sulfatides with different ceramide compositions gave a preferential binding of CS30 to sulfatide with d18:1-h24:0 ceramide. This ceramide species of sulfatide was also isolated from human small intestine and characterized by mass spectrometry and antibody binding. These studies implicate sulfatide as candidate receptor for mediating attachment of CS30-fimbriated ETEC to human and porcine small intestinal cells. Our findings may be a basis for designing receptor saccharide analogues for inhibition of the intestinal adhesion of CS30-expressing E. coli.Astrid Von MentzerDani ZalemZofia ChrienovaSusann TenebergTaylor & Francis Grouparticleenterotoxigenic e. colicolonization factor cs30microbial adhesioncarbohydrate bindingglycosphingolipid characterizationsulfatideInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 381-390 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	enterotoxigenic e. coli colonization factor cs30 microbial adhesion carbohydrate binding glycosphingolipid characterization sulfatide Infectious and parasitic diseases RC109-216
spellingShingle	enterotoxigenic e. coli colonization factor cs30 microbial adhesion carbohydrate binding glycosphingolipid characterization sulfatide Infectious and parasitic diseases RC109-216 Astrid Von Mentzer Dani Zalem Zofia Chrienova Susann Teneberg Colonization factor CS30 from enterotoxigenic Escherichia coli binds to sulfatide in human and porcine small intestine
description	The ability to adhere via colonization factors to specific receptors located on the intestinal mucosa is a key virulence factor in enterotoxigenic Escherichia coli (ETEC) pathogenesis. Here, the potential glycosphingolipid receptors of the novel human ETEC colonization factor CS30 were examined by binding of CS30-expressing bacteria to glycosphingolipids on thin-layer chromatograms. We thereby found a highly specific binding of CS30-expressing bacteria to a fast-migrating acid glycosphingolipid of human and porcine small intestine, while no binding was obtained with a mutant ETEC strain unable to express CS30 fimbriae. The CS30 binding glycosphingolipid from human small intestine was isolated and characterized by mass spectrometry as sulfatide (SO3-3Galβ1Cer). Comparative binding studies using sulfatides with different ceramide compositions gave a preferential binding of CS30 to sulfatide with d18:1-h24:0 ceramide. This ceramide species of sulfatide was also isolated from human small intestine and characterized by mass spectrometry and antibody binding. These studies implicate sulfatide as candidate receptor for mediating attachment of CS30-fimbriated ETEC to human and porcine small intestinal cells. Our findings may be a basis for designing receptor saccharide analogues for inhibition of the intestinal adhesion of CS30-expressing E. coli.
format	article
author	Astrid Von Mentzer Dani Zalem Zofia Chrienova Susann Teneberg
author_facet	Astrid Von Mentzer Dani Zalem Zofia Chrienova Susann Teneberg
author_sort	Astrid Von Mentzer
title	Colonization factor CS30 from enterotoxigenic Escherichia coli binds to sulfatide in human and porcine small intestine
title_short	Colonization factor CS30 from enterotoxigenic Escherichia coli binds to sulfatide in human and porcine small intestine
title_full	Colonization factor CS30 from enterotoxigenic Escherichia coli binds to sulfatide in human and porcine small intestine
title_fullStr	Colonization factor CS30 from enterotoxigenic Escherichia coli binds to sulfatide in human and porcine small intestine
title_full_unstemmed	Colonization factor CS30 from enterotoxigenic Escherichia coli binds to sulfatide in human and porcine small intestine
title_sort	colonization factor cs30 from enterotoxigenic escherichia coli binds to sulfatide in human and porcine small intestine
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/a428fc35df25481694a39ad273dced73
work_keys_str_mv	AT astridvonmentzer colonizationfactorcs30fromenterotoxigenicescherichiacolibindstosulfatideinhumanandporcinesmallintestine AT danizalem colonizationfactorcs30fromenterotoxigenicescherichiacolibindstosulfatideinhumanandporcinesmallintestine AT zofiachrienova colonizationfactorcs30fromenterotoxigenicescherichiacolibindstosulfatideinhumanandporcinesmallintestine AT susannteneberg colonizationfactorcs30fromenterotoxigenicescherichiacolibindstosulfatideinhumanandporcinesmallintestine
_version_	1718425500188147712

Colonization factor CS30 from enterotoxigenic Escherichia coli binds to sulfatide in human and porcine small intestine

Ejemplares similares