Transabdominal amniocentesis in expectant management of preterm premature rupture of membranes: A single center prospective study

Aims. The aim of this study was to evaluate the role of IL-6 point-of-care test in amniotic fluid obtained from serial amniocentesis in expectantly managed women with PPROM between 24 and 34 weeks of gestation. Methods. We conducted a prospective observational cohort study which included 62 pregnant...

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Autores principales:	Veronika Fulova, Eliska Hostinska, Martina Studnickova, Karel Huml, Jana Zapletalova, Jan Halek, Radovan Pilka
Formato:	article
Lenguaje:	EN
Publicado:	Palacký University Olomouc, Faculty of Medicine and Dentistry 2021
Materias:	pprom il-6 poin-of-care test amniocentesis Medicine R
Acceso en línea:	https://doaj.org/article/a429d6d60a7a4f37a8ce70b5a4c0ef67
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spelling	oai:doaj.org-article:a429d6d60a7a4f37a8ce70b5a4c0ef672021-11-29T08:47:12ZTransabdominal amniocentesis in expectant management of preterm premature rupture of membranes: A single center prospective study1213-81181804-752110.5507/bp.2020.041https://doaj.org/article/a429d6d60a7a4f37a8ce70b5a4c0ef672021-09-01T00:00:00Zhttps://biomed.papers.upol.cz/artkey/bio-202103-0011_transabdominal-amniocentesis-in-expectant-management-of-preterm-premature-rupture-of-membranes-a-single-center.phphttps://doaj.org/toc/1213-8118https://doaj.org/toc/1804-7521Aims. The aim of this study was to evaluate the role of IL-6 point-of-care test in amniotic fluid obtained from serial amniocentesis in expectantly managed women with PPROM between 24 and 34 weeks of gestation. Methods. We conducted a prospective observational cohort study which included 62 pregnant women with PPROM in gestational weeks between 22+0 and 34+0. Women aged >18 years were eligible if they presented with PPROM and a singleton pregnancy. Only women who delivered at >24.0 weeks were included in the study. In all women, the maternal blood sampling and a transabdominal amniocentesis were performed at the time of admission prior to the administration of corticosteroids, antibiotics, or tocolytics, to rule out signs of chorioamnionitis. Maternal temperature, maternal serum C-reactive protein (CRP) and white blood cell (WBC) counts were assayed every subsequent day until delivery. Amniotic fluid was used for the clinical assessment (IL-6 point-of-care test, identification of microorganisms in the amniotic fluid. After one week of expectant management of PPROM, second amniocentesis with amniotic fluid sampling was performed in patients who did not deliver. For all newborns, medical records regarding neonatal morbidity and mortality were reviewed. Results. In total, 62 women aged 19 to 41 years were recruited in the study. The mean gestational age at the time of PPROM was 31+0, the mean gestational age at labor was 32+1, and the median time from PPROM to childbirth was 112 h. IL-6 point-of-care test values above 1,000 pg/mL (positive Il-6 AMC) were found in 12 women (19.4%) with median interval from PPROM to childbirth 56 h (min-max: 6.4-288). IL-6 point-of-care test values below 1,000 pg/mL (negative Il-6 AMC) were found in 51 women (81.0%). The neonatal mortality rate was 1.9% and was associated with prematurity. Conclusion. The major clinical finding of our study is that serial transabdominal amniocentesis with Il-6 point-of-care test helps to identify a high inflammatory status in amniotic fluid in women with PPROM. Subsequent expectant management of women with PPROM does not lead to worsening of short-term neonatal outcomes.Veronika FulovaEliska HostinskaMartina StudnickovaKarel HumlJana ZapletalovaJan HalekRadovan PilkaPalacký University Olomouc, Faculty of Medicine and Dentistryarticleppromil-6poin-of-care testamniocentesisMedicineRENBiomedical Papers, Vol 165, Iss 3, Pp 305-315 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pprom il-6 poin-of-care test amniocentesis Medicine R
spellingShingle	pprom il-6 poin-of-care test amniocentesis Medicine R Veronika Fulova Eliska Hostinska Martina Studnickova Karel Huml Jana Zapletalova Jan Halek Radovan Pilka Transabdominal amniocentesis in expectant management of preterm premature rupture of membranes: A single center prospective study
description	Aims. The aim of this study was to evaluate the role of IL-6 point-of-care test in amniotic fluid obtained from serial amniocentesis in expectantly managed women with PPROM between 24 and 34 weeks of gestation. Methods. We conducted a prospective observational cohort study which included 62 pregnant women with PPROM in gestational weeks between 22+0 and 34+0. Women aged >18 years were eligible if they presented with PPROM and a singleton pregnancy. Only women who delivered at >24.0 weeks were included in the study. In all women, the maternal blood sampling and a transabdominal amniocentesis were performed at the time of admission prior to the administration of corticosteroids, antibiotics, or tocolytics, to rule out signs of chorioamnionitis. Maternal temperature, maternal serum C-reactive protein (CRP) and white blood cell (WBC) counts were assayed every subsequent day until delivery. Amniotic fluid was used for the clinical assessment (IL-6 point-of-care test, identification of microorganisms in the amniotic fluid. After one week of expectant management of PPROM, second amniocentesis with amniotic fluid sampling was performed in patients who did not deliver. For all newborns, medical records regarding neonatal morbidity and mortality were reviewed. Results. In total, 62 women aged 19 to 41 years were recruited in the study. The mean gestational age at the time of PPROM was 31+0, the mean gestational age at labor was 32+1, and the median time from PPROM to childbirth was 112 h. IL-6 point-of-care test values above 1,000 pg/mL (positive Il-6 AMC) were found in 12 women (19.4%) with median interval from PPROM to childbirth 56 h (min-max: 6.4-288). IL-6 point-of-care test values below 1,000 pg/mL (negative Il-6 AMC) were found in 51 women (81.0%). The neonatal mortality rate was 1.9% and was associated with prematurity. Conclusion. The major clinical finding of our study is that serial transabdominal amniocentesis with Il-6 point-of-care test helps to identify a high inflammatory status in amniotic fluid in women with PPROM. Subsequent expectant management of women with PPROM does not lead to worsening of short-term neonatal outcomes.
format	article
author	Veronika Fulova Eliska Hostinska Martina Studnickova Karel Huml Jana Zapletalova Jan Halek Radovan Pilka
author_facet	Veronika Fulova Eliska Hostinska Martina Studnickova Karel Huml Jana Zapletalova Jan Halek Radovan Pilka
author_sort	Veronika Fulova
title	Transabdominal amniocentesis in expectant management of preterm premature rupture of membranes: A single center prospective study
title_short	Transabdominal amniocentesis in expectant management of preterm premature rupture of membranes: A single center prospective study
title_full	Transabdominal amniocentesis in expectant management of preterm premature rupture of membranes: A single center prospective study
title_fullStr	Transabdominal amniocentesis in expectant management of preterm premature rupture of membranes: A single center prospective study
title_full_unstemmed	Transabdominal amniocentesis in expectant management of preterm premature rupture of membranes: A single center prospective study
title_sort	transabdominal amniocentesis in expectant management of preterm premature rupture of membranes: a single center prospective study
publisher	Palacký University Olomouc, Faculty of Medicine and Dentistry
publishDate	2021
url	https://doaj.org/article/a429d6d60a7a4f37a8ce70b5a4c0ef67
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Transabdominal amniocentesis in expectant management of preterm premature rupture of membranes: A single center prospective study

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