Gene circuit analysis of the terminal gap gene huckebein.

The early embryo of Drosophila melanogaster provides a powerful model system to study the role of genes in pattern formation. The gap gene network constitutes the first zygotic regulatory tier in the hierarchy of the segmentation genes involved in specifying the position of body segments. Here, we u...

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Autores principales:	Maksat Ashyraliyev, Ken Siggens, Hilde Janssens, Joke Blom, Michael Akam, Johannes Jaeger
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a42dbf11a7c6498d890341d47afd4538
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spelling	oai:doaj.org-article:a42dbf11a7c6498d890341d47afd45382021-12-02T19:57:53ZGene circuit analysis of the terminal gap gene huckebein.1553-734X1553-735810.1371/journal.pcbi.1000548https://doaj.org/article/a42dbf11a7c6498d890341d47afd45382009-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19876378/?tool=EBIhttps://doaj.org/toc/1553-734Xhttps://doaj.org/toc/1553-7358The early embryo of Drosophila melanogaster provides a powerful model system to study the role of genes in pattern formation. The gap gene network constitutes the first zygotic regulatory tier in the hierarchy of the segmentation genes involved in specifying the position of body segments. Here, we use an integrative, systems-level approach to investigate the regulatory effect of the terminal gap gene huckebein (hkb) on gap gene expression. We present quantitative expression data for the Hkb protein, which enable us to include hkb in gap gene circuit models. Gap gene circuits are mathematical models of gene networks used as computational tools to extract regulatory information from spatial expression data. This is achieved by fitting the model to gap gene expression patterns, in order to obtain estimates for regulatory parameters which predict a specific network topology. We show how considering variability in the data combined with analysis of parameter determinability significantly improves the biological relevance and consistency of the approach. Our models are in agreement with earlier results, which they extend in two important respects: First, we show that Hkb is involved in the regulation of the posterior hunchback (hb) domain, but does not have any other essential function. Specifically, Hkb is required for the anterior shift in the posterior border of this domain, which is now reproduced correctly in our models. Second, gap gene circuits presented here are able to reproduce mutants of terminal gap genes, while previously published models were unable to reproduce any null mutants correctly. As a consequence, our models now capture the expression dynamics of all posterior gap genes and some variational properties of the system correctly. This is an important step towards a better, quantitative understanding of the developmental and evolutionary dynamics of the gap gene network.Maksat AshyraliyevKen SiggensHilde JanssensJoke BlomMichael AkamJohannes JaegerPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Computational Biology, Vol 5, Iss 10, p e1000548 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Maksat Ashyraliyev Ken Siggens Hilde Janssens Joke Blom Michael Akam Johannes Jaeger Gene circuit analysis of the terminal gap gene huckebein.
description	The early embryo of Drosophila melanogaster provides a powerful model system to study the role of genes in pattern formation. The gap gene network constitutes the first zygotic regulatory tier in the hierarchy of the segmentation genes involved in specifying the position of body segments. Here, we use an integrative, systems-level approach to investigate the regulatory effect of the terminal gap gene huckebein (hkb) on gap gene expression. We present quantitative expression data for the Hkb protein, which enable us to include hkb in gap gene circuit models. Gap gene circuits are mathematical models of gene networks used as computational tools to extract regulatory information from spatial expression data. This is achieved by fitting the model to gap gene expression patterns, in order to obtain estimates for regulatory parameters which predict a specific network topology. We show how considering variability in the data combined with analysis of parameter determinability significantly improves the biological relevance and consistency of the approach. Our models are in agreement with earlier results, which they extend in two important respects: First, we show that Hkb is involved in the regulation of the posterior hunchback (hb) domain, but does not have any other essential function. Specifically, Hkb is required for the anterior shift in the posterior border of this domain, which is now reproduced correctly in our models. Second, gap gene circuits presented here are able to reproduce mutants of terminal gap genes, while previously published models were unable to reproduce any null mutants correctly. As a consequence, our models now capture the expression dynamics of all posterior gap genes and some variational properties of the system correctly. This is an important step towards a better, quantitative understanding of the developmental and evolutionary dynamics of the gap gene network.
format	article
author	Maksat Ashyraliyev Ken Siggens Hilde Janssens Joke Blom Michael Akam Johannes Jaeger
author_facet	Maksat Ashyraliyev Ken Siggens Hilde Janssens Joke Blom Michael Akam Johannes Jaeger
author_sort	Maksat Ashyraliyev
title	Gene circuit analysis of the terminal gap gene huckebein.
title_short	Gene circuit analysis of the terminal gap gene huckebein.
title_full	Gene circuit analysis of the terminal gap gene huckebein.
title_fullStr	Gene circuit analysis of the terminal gap gene huckebein.
title_full_unstemmed	Gene circuit analysis of the terminal gap gene huckebein.
title_sort	gene circuit analysis of the terminal gap gene huckebein.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/a42dbf11a7c6498d890341d47afd4538
work_keys_str_mv	AT maksatashyraliyev genecircuitanalysisoftheterminalgapgenehuckebein AT kensiggens genecircuitanalysisoftheterminalgapgenehuckebein AT hildejanssens genecircuitanalysisoftheterminalgapgenehuckebein AT jokeblom genecircuitanalysisoftheterminalgapgenehuckebein AT michaelakam genecircuitanalysisoftheterminalgapgenehuckebein AT johannesjaeger genecircuitanalysisoftheterminalgapgenehuckebein
_version_	1718375794178260992

Gene circuit analysis of the terminal gap gene huckebein.

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