Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters

Analogues of α-ketoglutarate are used in many cellular studies but assumptions are made about cellular uptake. Here, the authors show that esterified analogues rapidly hydrolyse in aqueous medium resulting in an analogue which can be quickly taken up by many cell lines, contrary to prevailing assump...

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Autores principales:	Seth J. Parker, Joel Encarnación-Rosado, Kate E. R. Hollinshead, David M. Hollinshead, Leonard J. Ash, Juan A. K. Rossi, Elaine Y. Lin, Albert S. W. Sohn, Mark R. Philips, Drew R. Jones, Alec C. Kimmelman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/a430c84ac3014555b5b20af966982f02
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spelling	oai:doaj.org-article:a430c84ac3014555b5b20af966982f022021-12-02T16:28:52ZSpontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters10.1038/s41467-021-25228-92041-1723https://doaj.org/article/a430c84ac3014555b5b20af966982f022021-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25228-9https://doaj.org/toc/2041-1723Analogues of α-ketoglutarate are used in many cellular studies but assumptions are made about cellular uptake. Here, the authors show that esterified analogues rapidly hydrolyse in aqueous medium resulting in an analogue which can be quickly taken up by many cell lines, contrary to prevailing assumptions.Seth J. ParkerJoel Encarnación-RosadoKate E. R. HollinsheadDavid M. HollinsheadLeonard J. AshJuan A. K. RossiElaine Y. LinAlbert S. W. SohnMark R. PhilipsDrew R. JonesAlec C. KimmelmanNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Seth J. Parker Joel Encarnación-Rosado Kate E. R. Hollinshead David M. Hollinshead Leonard J. Ash Juan A. K. Rossi Elaine Y. Lin Albert S. W. Sohn Mark R. Philips Drew R. Jones Alec C. Kimmelman Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters
description	Analogues of α-ketoglutarate are used in many cellular studies but assumptions are made about cellular uptake. Here, the authors show that esterified analogues rapidly hydrolyse in aqueous medium resulting in an analogue which can be quickly taken up by many cell lines, contrary to prevailing assumptions.
format	article
author	Seth J. Parker Joel Encarnación-Rosado Kate E. R. Hollinshead David M. Hollinshead Leonard J. Ash Juan A. K. Rossi Elaine Y. Lin Albert S. W. Sohn Mark R. Philips Drew R. Jones Alec C. Kimmelman
author_facet	Seth J. Parker Joel Encarnación-Rosado Kate E. R. Hollinshead David M. Hollinshead Leonard J. Ash Juan A. K. Rossi Elaine Y. Lin Albert S. W. Sohn Mark R. Philips Drew R. Jones Alec C. Kimmelman
author_sort	Seth J. Parker
title	Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters
title_short	Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters
title_full	Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters
title_fullStr	Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters
title_full_unstemmed	Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters
title_sort	spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a430c84ac3014555b5b20af966982f02
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Spontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters

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