Focused Screening and Treatment (FSAT): a PCR-based strategy to detect malaria parasite carriers and contain drug resistant P. falciparum, Pailin, Cambodia.

Recent studies have shown that Plasmodium falciparum malaria parasites in Pailin province, along the border between Thailand and Cambodia, have become resistant to artemisinin derivatives. To better define the epidemiology of P. falciparum populations and to assess the risk of the possible spread of...

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Autores principales:	Stefan Hoyer, Sokomar Nguon, Saorin Kim, Najibullah Habib, Nimol Khim, Sarorn Sum, Eva-Maria Christophel, Steven Bjorge, Andrew Thomson, Sim Kheng, Nguon Chea, Sovann Yok, Samphornarann Top, Seyha Ros, Uth Sophal, Michelle M Thompson, Steve Mellor, Frédéric Ariey, Benoit Witkowski, Chhiang Yeang, Shunmay Yeung, Socheat Duong, Robert D Newman, Didier Menard
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a431e3e98897436e9e09bef3d6c71638
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spelling	oai:doaj.org-article:a431e3e98897436e9e09bef3d6c716382021-11-18T08:13:34ZFocused Screening and Treatment (FSAT): a PCR-based strategy to detect malaria parasite carriers and contain drug resistant P. falciparum, Pailin, Cambodia.1932-620310.1371/journal.pone.0045797https://doaj.org/article/a431e3e98897436e9e09bef3d6c716382012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23049687/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Recent studies have shown that Plasmodium falciparum malaria parasites in Pailin province, along the border between Thailand and Cambodia, have become resistant to artemisinin derivatives. To better define the epidemiology of P. falciparum populations and to assess the risk of the possible spread of these parasites outside Pailin, a new epidemiological tool named "Focused Screening and Treatment" (FSAT), based on active molecular detection of asymptomatic parasite carriers was introduced in 2010. Cross-sectional malariometric surveys using PCR were carried out in 20 out of 109 villages in Pailin province. Individuals detected as P. falciparum carriers were treated with atovaquone-proguanil combination plus a single dose of primaquine if the patient was non-G6PD deficient. Interviews were conducted to elicit history of cross-border travel that might contribute to the spread of artemisinin-resistant parasites. After directly observed treatment, patients were followed up and re-examined on day 7 and day 28. Among 6931 individuals screened, prevalence of P. falciparum carriers was less than 1%, of whom 96% were asymptomatic. Only 1.6% of the individuals had a travel history or plans to go outside Cambodia, with none of those tested being positive for P. falciparum. Retrospective analysis, using 2010 routine surveillance data, showed significant differences in the prevalence of asymptomatic carriers discovered by FSAT between villages classified as "high risk" and "low risk" based on malaria incidence data. All positive individuals treated and followed-up until day 28 were cured. No mutant-type allele related to atovaquone resistance was found. FSAT is a potentially useful tool to detect, treat and track clusters of asymptomatic carriers of P. falciparum along with providing valuable epidemiological information regarding cross-border movements of potential malaria parasite carriers and parasite gene flow.Stefan HoyerSokomar NguonSaorin KimNajibullah HabibNimol KhimSarorn SumEva-Maria ChristophelSteven BjorgeAndrew ThomsonSim KhengNguon CheaSovann YokSamphornarann TopSeyha RosUth SophalMichelle M ThompsonSteve MellorFrédéric ArieyBenoit WitkowskiChhiang YeangShunmay YeungSocheat DuongRobert D NewmanDidier MenardPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e45797 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefan Hoyer Sokomar Nguon Saorin Kim Najibullah Habib Nimol Khim Sarorn Sum Eva-Maria Christophel Steven Bjorge Andrew Thomson Sim Kheng Nguon Chea Sovann Yok Samphornarann Top Seyha Ros Uth Sophal Michelle M Thompson Steve Mellor Frédéric Ariey Benoit Witkowski Chhiang Yeang Shunmay Yeung Socheat Duong Robert D Newman Didier Menard Focused Screening and Treatment (FSAT): a PCR-based strategy to detect malaria parasite carriers and contain drug resistant P. falciparum, Pailin, Cambodia.
description	Recent studies have shown that Plasmodium falciparum malaria parasites in Pailin province, along the border between Thailand and Cambodia, have become resistant to artemisinin derivatives. To better define the epidemiology of P. falciparum populations and to assess the risk of the possible spread of these parasites outside Pailin, a new epidemiological tool named "Focused Screening and Treatment" (FSAT), based on active molecular detection of asymptomatic parasite carriers was introduced in 2010. Cross-sectional malariometric surveys using PCR were carried out in 20 out of 109 villages in Pailin province. Individuals detected as P. falciparum carriers were treated with atovaquone-proguanil combination plus a single dose of primaquine if the patient was non-G6PD deficient. Interviews were conducted to elicit history of cross-border travel that might contribute to the spread of artemisinin-resistant parasites. After directly observed treatment, patients were followed up and re-examined on day 7 and day 28. Among 6931 individuals screened, prevalence of P. falciparum carriers was less than 1%, of whom 96% were asymptomatic. Only 1.6% of the individuals had a travel history or plans to go outside Cambodia, with none of those tested being positive for P. falciparum. Retrospective analysis, using 2010 routine surveillance data, showed significant differences in the prevalence of asymptomatic carriers discovered by FSAT between villages classified as "high risk" and "low risk" based on malaria incidence data. All positive individuals treated and followed-up until day 28 were cured. No mutant-type allele related to atovaquone resistance was found. FSAT is a potentially useful tool to detect, treat and track clusters of asymptomatic carriers of P. falciparum along with providing valuable epidemiological information regarding cross-border movements of potential malaria parasite carriers and parasite gene flow.
format	article
author	Stefan Hoyer Sokomar Nguon Saorin Kim Najibullah Habib Nimol Khim Sarorn Sum Eva-Maria Christophel Steven Bjorge Andrew Thomson Sim Kheng Nguon Chea Sovann Yok Samphornarann Top Seyha Ros Uth Sophal Michelle M Thompson Steve Mellor Frédéric Ariey Benoit Witkowski Chhiang Yeang Shunmay Yeung Socheat Duong Robert D Newman Didier Menard
author_facet	Stefan Hoyer Sokomar Nguon Saorin Kim Najibullah Habib Nimol Khim Sarorn Sum Eva-Maria Christophel Steven Bjorge Andrew Thomson Sim Kheng Nguon Chea Sovann Yok Samphornarann Top Seyha Ros Uth Sophal Michelle M Thompson Steve Mellor Frédéric Ariey Benoit Witkowski Chhiang Yeang Shunmay Yeung Socheat Duong Robert D Newman Didier Menard
author_sort	Stefan Hoyer
title	Focused Screening and Treatment (FSAT): a PCR-based strategy to detect malaria parasite carriers and contain drug resistant P. falciparum, Pailin, Cambodia.
title_short	Focused Screening and Treatment (FSAT): a PCR-based strategy to detect malaria parasite carriers and contain drug resistant P. falciparum, Pailin, Cambodia.
title_full	Focused Screening and Treatment (FSAT): a PCR-based strategy to detect malaria parasite carriers and contain drug resistant P. falciparum, Pailin, Cambodia.
title_fullStr	Focused Screening and Treatment (FSAT): a PCR-based strategy to detect malaria parasite carriers and contain drug resistant P. falciparum, Pailin, Cambodia.
title_full_unstemmed	Focused Screening and Treatment (FSAT): a PCR-based strategy to detect malaria parasite carriers and contain drug resistant P. falciparum, Pailin, Cambodia.
title_sort	focused screening and treatment (fsat): a pcr-based strategy to detect malaria parasite carriers and contain drug resistant p. falciparum, pailin, cambodia.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/a431e3e98897436e9e09bef3d6c71638
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Focused Screening and Treatment (FSAT): a PCR-based strategy to detect malaria parasite carriers and contain drug resistant P. falciparum, Pailin, Cambodia.

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