A thiol probe for measuring unfolded protein load and proteostasis in cells
Proteostasis is maintained through a number of molecular mechanisms, some of which function to protect the folded state of proteins. Here the authors demonstrate the use of TPE-MI in a fluorigenic dye assay for the quantitation of unfolded proteins that can be used to assess proteostasis on a cellul...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2017
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/a4333004f228480fbf7846ca619a3233 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:a4333004f228480fbf7846ca619a3233 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:a4333004f228480fbf7846ca619a32332021-12-02T14:42:38ZA thiol probe for measuring unfolded protein load and proteostasis in cells10.1038/s41467-017-00203-52041-1723https://doaj.org/article/a4333004f228480fbf7846ca619a32332017-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-00203-5https://doaj.org/toc/2041-1723Proteostasis is maintained through a number of molecular mechanisms, some of which function to protect the folded state of proteins. Here the authors demonstrate the use of TPE-MI in a fluorigenic dye assay for the quantitation of unfolded proteins that can be used to assess proteostasis on a cellular or proteome scale.Moore Z. ChenNagaraj S. MoilyJessica L. BridgfordRebecca J. WoodMona RadwanTrevor A. SmithZhegang SongBen Zhong TangLeann TilleyXiaohong XuGavin E. ReidMahmoud A. PouladiYuning HongDanny M. HattersNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-11 (2017) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Moore Z. Chen Nagaraj S. Moily Jessica L. Bridgford Rebecca J. Wood Mona Radwan Trevor A. Smith Zhegang Song Ben Zhong Tang Leann Tilley Xiaohong Xu Gavin E. Reid Mahmoud A. Pouladi Yuning Hong Danny M. Hatters A thiol probe for measuring unfolded protein load and proteostasis in cells |
| description |
Proteostasis is maintained through a number of molecular mechanisms, some of which function to protect the folded state of proteins. Here the authors demonstrate the use of TPE-MI in a fluorigenic dye assay for the quantitation of unfolded proteins that can be used to assess proteostasis on a cellular or proteome scale. |
| format |
article |
| author |
Moore Z. Chen Nagaraj S. Moily Jessica L. Bridgford Rebecca J. Wood Mona Radwan Trevor A. Smith Zhegang Song Ben Zhong Tang Leann Tilley Xiaohong Xu Gavin E. Reid Mahmoud A. Pouladi Yuning Hong Danny M. Hatters |
| author_facet |
Moore Z. Chen Nagaraj S. Moily Jessica L. Bridgford Rebecca J. Wood Mona Radwan Trevor A. Smith Zhegang Song Ben Zhong Tang Leann Tilley Xiaohong Xu Gavin E. Reid Mahmoud A. Pouladi Yuning Hong Danny M. Hatters |
| author_sort |
Moore Z. Chen |
| title |
A thiol probe for measuring unfolded protein load and proteostasis in cells |
| title_short |
A thiol probe for measuring unfolded protein load and proteostasis in cells |
| title_full |
A thiol probe for measuring unfolded protein load and proteostasis in cells |
| title_fullStr |
A thiol probe for measuring unfolded protein load and proteostasis in cells |
| title_full_unstemmed |
A thiol probe for measuring unfolded protein load and proteostasis in cells |
| title_sort |
thiol probe for measuring unfolded protein load and proteostasis in cells |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/a4333004f228480fbf7846ca619a3233 |
| work_keys_str_mv |
AT moorezchen athiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT nagarajsmoily athiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT jessicalbridgford athiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT rebeccajwood athiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT monaradwan athiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT trevorasmith athiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT zhegangsong athiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT benzhongtang athiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT leanntilley athiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT xiaohongxu athiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT gavinereid athiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT mahmoudapouladi athiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT yuninghong athiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT dannymhatters athiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT moorezchen thiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT nagarajsmoily thiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT jessicalbridgford thiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT rebeccajwood thiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT monaradwan thiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT trevorasmith thiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT zhegangsong thiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT benzhongtang thiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT leanntilley thiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT xiaohongxu thiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT gavinereid thiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT mahmoudapouladi thiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT yuninghong thiolprobeformeasuringunfoldedproteinloadandproteostasisincells AT dannymhatters thiolprobeformeasuringunfoldedproteinloadandproteostasisincells |
| _version_ |
1718389649798332416 |