Differential Circulating MicroRNA Expression in Age-Related Macular Degeneration

This study explored the expression of several miRNAs reported to be deregulated in age-related macular degeneration (AMD). Total RNA was isolated from sera from patients with dry AMD (n = 12), wet AMD (n = 14), and controls (n = 10). Forty-two...

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Autores principales:	Hanan ElShelmani, Ian Brennan, David J. Kelly, David Keegan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microRNA age-related macular degeneration biomarkers serum dry AMD wet AMD Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/a434f79be21b4310bcd04da32ae1e39a
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spelling	oai:doaj.org-article:a434f79be21b4310bcd04da32ae1e39a2021-11-25T17:55:23ZDifferential Circulating MicroRNA Expression in Age-Related Macular Degeneration10.3390/ijms2222123211422-00671661-6596https://doaj.org/article/a434f79be21b4310bcd04da32ae1e39a2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12321https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067This study explored the expression of several miRNAs reported to be deregulated in age-related macular degeneration (AMD). Total RNA was isolated from sera from patients with dry AMD (<i>n</i> = 12), wet AMD (<i>n</i> = 14), and controls (<i>n</i> = 10). Forty-two previously investigated miRNAs were selected based on published data and their role in AMD pathogenesis, such as angiogenic and inflammatory effects, and were co-analysed using a miRCURY LNA miRNA SYBR<sup>®</sup> Green PCR kit via quantitative real-time polymerase chain reaction (qRT-PCR) to validate their presence. Unsupervised hierarchical clustering indicated that AMD serum specimens have a different miRNA profile to healthy controls. We successfully validated the differentially regulated miRNAs in serum from AMD patients versus controls. Eight miRNAs (hsa-let-7a-5p, hsa-let-7d-5p, hsa-miR-23a-3p, hsa-miR-301a-3p, hsa-miR-361-5p, hsa-miR-27b-3p, hsa-miR-874-3p, hsa-miR-19b-1-5p) showed higher expression in the serum of dry AMD patients than wet AMD patients and compared with healthy controls. Increased quantities of certain miRNAs in the serum of AMD patients indicate that these miRNAs could potentially serve as diagnostic AMD biomarkers and might be used as future AMD treatment targets. The discovery of significant serum miRNA biomarkers in AMD patients would provide an easy screening tool for at-risk populations.Hanan ElShelmaniIan BrennanDavid J. KellyDavid KeeganMDPI AGarticlemicroRNAage-related macular degenerationbiomarkersserumdry AMDwet AMDBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12321, p 12321 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microRNA age-related macular degeneration biomarkers serum dry AMD wet AMD Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	microRNA age-related macular degeneration biomarkers serum dry AMD wet AMD Biology (General) QH301-705.5 Chemistry QD1-999 Hanan ElShelmani Ian Brennan David J. Kelly David Keegan Differential Circulating MicroRNA Expression in Age-Related Macular Degeneration
description	This study explored the expression of several miRNAs reported to be deregulated in age-related macular degeneration (AMD). Total RNA was isolated from sera from patients with dry AMD (<i>n</i> = 12), wet AMD (<i>n</i> = 14), and controls (<i>n</i> = 10). Forty-two previously investigated miRNAs were selected based on published data and their role in AMD pathogenesis, such as angiogenic and inflammatory effects, and were co-analysed using a miRCURY LNA miRNA SYBR<sup>®</sup> Green PCR kit via quantitative real-time polymerase chain reaction (qRT-PCR) to validate their presence. Unsupervised hierarchical clustering indicated that AMD serum specimens have a different miRNA profile to healthy controls. We successfully validated the differentially regulated miRNAs in serum from AMD patients versus controls. Eight miRNAs (hsa-let-7a-5p, hsa-let-7d-5p, hsa-miR-23a-3p, hsa-miR-301a-3p, hsa-miR-361-5p, hsa-miR-27b-3p, hsa-miR-874-3p, hsa-miR-19b-1-5p) showed higher expression in the serum of dry AMD patients than wet AMD patients and compared with healthy controls. Increased quantities of certain miRNAs in the serum of AMD patients indicate that these miRNAs could potentially serve as diagnostic AMD biomarkers and might be used as future AMD treatment targets. The discovery of significant serum miRNA biomarkers in AMD patients would provide an easy screening tool for at-risk populations.
format	article
author	Hanan ElShelmani Ian Brennan David J. Kelly David Keegan
author_facet	Hanan ElShelmani Ian Brennan David J. Kelly David Keegan
author_sort	Hanan ElShelmani
title	Differential Circulating MicroRNA Expression in Age-Related Macular Degeneration
title_short	Differential Circulating MicroRNA Expression in Age-Related Macular Degeneration
title_full	Differential Circulating MicroRNA Expression in Age-Related Macular Degeneration
title_fullStr	Differential Circulating MicroRNA Expression in Age-Related Macular Degeneration
title_full_unstemmed	Differential Circulating MicroRNA Expression in Age-Related Macular Degeneration
title_sort	differential circulating microrna expression in age-related macular degeneration
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a434f79be21b4310bcd04da32ae1e39a
work_keys_str_mv	AT hananelshelmani differentialcirculatingmicrornaexpressioninagerelatedmaculardegeneration AT ianbrennan differentialcirculatingmicrornaexpressioninagerelatedmaculardegeneration AT davidjkelly differentialcirculatingmicrornaexpressioninagerelatedmaculardegeneration AT davidkeegan differentialcirculatingmicrornaexpressioninagerelatedmaculardegeneration
_version_	1718411877399134208

Differential Circulating MicroRNA Expression in Age-Related Macular Degeneration

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