Differential Circulating MicroRNA Expression in Age-Related Macular Degeneration
This study explored the expression of several miRNAs reported to be deregulated in age-related macular degeneration (AMD). Total RNA was isolated from sera from patients with dry AMD (<i>n</i> = 12), wet AMD (<i>n</i> = 14), and controls (<i>n</i> = 10). Forty-two...
Guardado en:
Autores principales: | , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
MDPI AG
2021
|
Materias: | |
Acceso en línea: | https://doaj.org/article/a434f79be21b4310bcd04da32ae1e39a |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:a434f79be21b4310bcd04da32ae1e39a |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:a434f79be21b4310bcd04da32ae1e39a2021-11-25T17:55:23ZDifferential Circulating MicroRNA Expression in Age-Related Macular Degeneration10.3390/ijms2222123211422-00671661-6596https://doaj.org/article/a434f79be21b4310bcd04da32ae1e39a2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12321https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067This study explored the expression of several miRNAs reported to be deregulated in age-related macular degeneration (AMD). Total RNA was isolated from sera from patients with dry AMD (<i>n</i> = 12), wet AMD (<i>n</i> = 14), and controls (<i>n</i> = 10). Forty-two previously investigated miRNAs were selected based on published data and their role in AMD pathogenesis, such as angiogenic and inflammatory effects, and were co-analysed using a miRCURY LNA miRNA SYBR<sup>®</sup> Green PCR kit via quantitative real-time polymerase chain reaction (qRT-PCR) to validate their presence. Unsupervised hierarchical clustering indicated that AMD serum specimens have a different miRNA profile to healthy controls. We successfully validated the differentially regulated miRNAs in serum from AMD patients versus controls. Eight miRNAs (hsa-let-7a-5p, hsa-let-7d-5p, hsa-miR-23a-3p, hsa-miR-301a-3p, hsa-miR-361-5p, hsa-miR-27b-3p, hsa-miR-874-3p, hsa-miR-19b-1-5p) showed higher expression in the serum of dry AMD patients than wet AMD patients and compared with healthy controls. Increased quantities of certain miRNAs in the serum of AMD patients indicate that these miRNAs could potentially serve as diagnostic AMD biomarkers and might be used as future AMD treatment targets. The discovery of significant serum miRNA biomarkers in AMD patients would provide an easy screening tool for at-risk populations.Hanan ElShelmaniIan BrennanDavid J. KellyDavid KeeganMDPI AGarticlemicroRNAage-related macular degenerationbiomarkersserumdry AMDwet AMDBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12321, p 12321 (2021) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
microRNA age-related macular degeneration biomarkers serum dry AMD wet AMD Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
microRNA age-related macular degeneration biomarkers serum dry AMD wet AMD Biology (General) QH301-705.5 Chemistry QD1-999 Hanan ElShelmani Ian Brennan David J. Kelly David Keegan Differential Circulating MicroRNA Expression in Age-Related Macular Degeneration |
description |
This study explored the expression of several miRNAs reported to be deregulated in age-related macular degeneration (AMD). Total RNA was isolated from sera from patients with dry AMD (<i>n</i> = 12), wet AMD (<i>n</i> = 14), and controls (<i>n</i> = 10). Forty-two previously investigated miRNAs were selected based on published data and their role in AMD pathogenesis, such as angiogenic and inflammatory effects, and were co-analysed using a miRCURY LNA miRNA SYBR<sup>®</sup> Green PCR kit via quantitative real-time polymerase chain reaction (qRT-PCR) to validate their presence. Unsupervised hierarchical clustering indicated that AMD serum specimens have a different miRNA profile to healthy controls. We successfully validated the differentially regulated miRNAs in serum from AMD patients versus controls. Eight miRNAs (hsa-let-7a-5p, hsa-let-7d-5p, hsa-miR-23a-3p, hsa-miR-301a-3p, hsa-miR-361-5p, hsa-miR-27b-3p, hsa-miR-874-3p, hsa-miR-19b-1-5p) showed higher expression in the serum of dry AMD patients than wet AMD patients and compared with healthy controls. Increased quantities of certain miRNAs in the serum of AMD patients indicate that these miRNAs could potentially serve as diagnostic AMD biomarkers and might be used as future AMD treatment targets. The discovery of significant serum miRNA biomarkers in AMD patients would provide an easy screening tool for at-risk populations. |
format |
article |
author |
Hanan ElShelmani Ian Brennan David J. Kelly David Keegan |
author_facet |
Hanan ElShelmani Ian Brennan David J. Kelly David Keegan |
author_sort |
Hanan ElShelmani |
title |
Differential Circulating MicroRNA Expression in Age-Related Macular Degeneration |
title_short |
Differential Circulating MicroRNA Expression in Age-Related Macular Degeneration |
title_full |
Differential Circulating MicroRNA Expression in Age-Related Macular Degeneration |
title_fullStr |
Differential Circulating MicroRNA Expression in Age-Related Macular Degeneration |
title_full_unstemmed |
Differential Circulating MicroRNA Expression in Age-Related Macular Degeneration |
title_sort |
differential circulating microrna expression in age-related macular degeneration |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doaj.org/article/a434f79be21b4310bcd04da32ae1e39a |
work_keys_str_mv |
AT hananelshelmani differentialcirculatingmicrornaexpressioninagerelatedmaculardegeneration AT ianbrennan differentialcirculatingmicrornaexpressioninagerelatedmaculardegeneration AT davidjkelly differentialcirculatingmicrornaexpressioninagerelatedmaculardegeneration AT davidkeegan differentialcirculatingmicrornaexpressioninagerelatedmaculardegeneration |
_version_ |
1718411877399134208 |