Mg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium

A synthetic microbial consortium called Effective Microorganisms (EM) consists mainly of photosynthetic bacteria, lactic acid bacteria and yeast. Various effects of EM∙XGOLD, a health drink produced by EM, on life cycle of Dictyostelium discoideum were described previously. Here, we report our attem...

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Autores principales:	Toru Higashinakagawa, Haruhisa Kikuchi, Hidekazu Kuwayama
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a43542aedbae4c9a9bf18ff23ddb7c02
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spelling	oai:doaj.org-article:a43542aedbae4c9a9bf18ff23ddb7c022021-11-11T06:44:16ZMg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium1932-6203https://doaj.org/article/a43542aedbae4c9a9bf18ff23ddb7c022021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565762/?tool=EBIhttps://doaj.org/toc/1932-6203A synthetic microbial consortium called Effective Microorganisms (EM) consists mainly of photosynthetic bacteria, lactic acid bacteria and yeast. Various effects of EM∙XGOLD, a health drink produced by EM, on life cycle of Dictyostelium discoideum were described previously. Here, we report our attempt to identify the active principle, termed EMF, that brought about the observed effects. Throughout the purification processes, the presence of the active principle was monitored by promoted fruiting body formation. By liquid-liquid separation the activity was recovered in aqueous phase, which, after concentration, was further subjected to reverse-phase column chromatography. No activity was detected in any eluant, while almost all the activity was recovered in residual insoluble material. The application of conventional organic chemistry procedures to the residual fraction did not lead to any informative results. Acid treatment of the insoluble material produced air bubbles, suggesting it to be composed of some inorganic carbonate. Viewed under scanning electronmicroscope, the residue revealed spherical particles of μm size range. Energy Dispersive X-ray (EDX) Spectroscopy pointed to the existence, on the surface of the particles, of magnesium and, to a certain extent, of potassium. In separate experiments, acid treatment and alkali neutralization of EM∙XGOLD completely wiped out the stimulatory activity of fruiting body formation. These lines of evidence indicate these Mg, K-containing microparticles to be an active principle of EM culture extract. How these particles exert their effect is currently under intensive investigation.Toru HigashinakagawaHaruhisa KikuchiHidekazu KuwayamaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Toru Higashinakagawa Haruhisa Kikuchi Hidekazu Kuwayama Mg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium
description	A synthetic microbial consortium called Effective Microorganisms (EM) consists mainly of photosynthetic bacteria, lactic acid bacteria and yeast. Various effects of EM∙XGOLD, a health drink produced by EM, on life cycle of Dictyostelium discoideum were described previously. Here, we report our attempt to identify the active principle, termed EMF, that brought about the observed effects. Throughout the purification processes, the presence of the active principle was monitored by promoted fruiting body formation. By liquid-liquid separation the activity was recovered in aqueous phase, which, after concentration, was further subjected to reverse-phase column chromatography. No activity was detected in any eluant, while almost all the activity was recovered in residual insoluble material. The application of conventional organic chemistry procedures to the residual fraction did not lead to any informative results. Acid treatment of the insoluble material produced air bubbles, suggesting it to be composed of some inorganic carbonate. Viewed under scanning electronmicroscope, the residue revealed spherical particles of μm size range. Energy Dispersive X-ray (EDX) Spectroscopy pointed to the existence, on the surface of the particles, of magnesium and, to a certain extent, of potassium. In separate experiments, acid treatment and alkali neutralization of EM∙XGOLD completely wiped out the stimulatory activity of fruiting body formation. These lines of evidence indicate these Mg, K-containing microparticles to be an active principle of EM culture extract. How these particles exert their effect is currently under intensive investigation.
format	article
author	Toru Higashinakagawa Haruhisa Kikuchi Hidekazu Kuwayama
author_facet	Toru Higashinakagawa Haruhisa Kikuchi Hidekazu Kuwayama
author_sort	Toru Higashinakagawa
title	Mg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium
title_short	Mg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium
title_full	Mg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium
title_fullStr	Mg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium
title_full_unstemmed	Mg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium
title_sort	mg, k-containing microparticle: a possible active principle of a culture extract produced by a microbial consortium
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a43542aedbae4c9a9bf18ff23ddb7c02
work_keys_str_mv	AT toruhigashinakagawa mgkcontainingmicroparticleapossibleactiveprincipleofacultureextractproducedbyamicrobialconsortium AT haruhisakikuchi mgkcontainingmicroparticleapossibleactiveprincipleofacultureextractproducedbyamicrobialconsortium AT hidekazukuwayama mgkcontainingmicroparticleapossibleactiveprincipleofacultureextractproducedbyamicrobialconsortium
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Mg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium

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