Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach
Abstract Cold environments dominate Earth’s biosphere, hosting complex microbial communities with the ability to thrive at low temperatures. However, the underlying molecular mechanisms and the metabolic pathways involved in bacterial cold-adaptation mechanisms are still not fully understood. Herein...
Guardado en:
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2017
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/a437404c4f384932a1bb28a3e4a0d789 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:a437404c4f384932a1bb28a3e4a0d789 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:a437404c4f384932a1bb28a3e4a0d7892021-12-02T15:05:37ZEcology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach10.1038/s41598-017-00876-42045-2322https://doaj.org/article/a437404c4f384932a1bb28a3e4a0d7892017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00876-4https://doaj.org/toc/2045-2322Abstract Cold environments dominate Earth’s biosphere, hosting complex microbial communities with the ability to thrive at low temperatures. However, the underlying molecular mechanisms and the metabolic pathways involved in bacterial cold-adaptation mechanisms are still not fully understood. Herein, we assessed the metabolic features of the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 (PhTAC125), a model organism for cold-adaptation, at both 4 °C and 15 °C, by integrating genomic and phenomic (high-throughput phenotyping) data and comparing the obtained results to the taxonomically related Antarctic bacterium Pseudoalteromonas sp. TB41 (PspTB41). Although the genome size of PspTB41 is considerably larger than PhTAC125, the higher number of genes did not reflect any higher metabolic versatility at 4 °C as compared to PhTAC125. Remarkably, protein S-thiolation regulated by glutathione and glutathionylspermidine appeared to be a new possible mechanism for cold adaptation in PhTAC125. More in general, this study represents an example of how ‘multi-omic’ information might potentially contribute in filling the gap between genotypic and phenotypic features related to cold-adaptation mechanisms in bacteria.Stefano MocaliCarolina ChielliniArturo FabianiSilvia DecuzziDonatella de PascaleErmenegilda ParrilliMaria Luisa TutinoElena PerrinEmanuele BosiMarco FondiAngelina Lo GiudiceRenato FaniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Stefano Mocali Carolina Chiellini Arturo Fabiani Silvia Decuzzi Donatella de Pascale Ermenegilda Parrilli Maria Luisa Tutino Elena Perrin Emanuele Bosi Marco Fondi Angelina Lo Giudice Renato Fani Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach |
| description |
Abstract Cold environments dominate Earth’s biosphere, hosting complex microbial communities with the ability to thrive at low temperatures. However, the underlying molecular mechanisms and the metabolic pathways involved in bacterial cold-adaptation mechanisms are still not fully understood. Herein, we assessed the metabolic features of the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 (PhTAC125), a model organism for cold-adaptation, at both 4 °C and 15 °C, by integrating genomic and phenomic (high-throughput phenotyping) data and comparing the obtained results to the taxonomically related Antarctic bacterium Pseudoalteromonas sp. TB41 (PspTB41). Although the genome size of PspTB41 is considerably larger than PhTAC125, the higher number of genes did not reflect any higher metabolic versatility at 4 °C as compared to PhTAC125. Remarkably, protein S-thiolation regulated by glutathione and glutathionylspermidine appeared to be a new possible mechanism for cold adaptation in PhTAC125. More in general, this study represents an example of how ‘multi-omic’ information might potentially contribute in filling the gap between genotypic and phenotypic features related to cold-adaptation mechanisms in bacteria. |
| format |
article |
| author |
Stefano Mocali Carolina Chiellini Arturo Fabiani Silvia Decuzzi Donatella de Pascale Ermenegilda Parrilli Maria Luisa Tutino Elena Perrin Emanuele Bosi Marco Fondi Angelina Lo Giudice Renato Fani |
| author_facet |
Stefano Mocali Carolina Chiellini Arturo Fabiani Silvia Decuzzi Donatella de Pascale Ermenegilda Parrilli Maria Luisa Tutino Elena Perrin Emanuele Bosi Marco Fondi Angelina Lo Giudice Renato Fani |
| author_sort |
Stefano Mocali |
| title |
Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach |
| title_short |
Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach |
| title_full |
Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach |
| title_fullStr |
Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach |
| title_full_unstemmed |
Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach |
| title_sort |
ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/a437404c4f384932a1bb28a3e4a0d789 |
| work_keys_str_mv |
AT stefanomocali ecologyofcoldenvironmentsnewinsightsofbacterialmetabolicadaptationthroughanintegratedgenomicphenomicapproach AT carolinachiellini ecologyofcoldenvironmentsnewinsightsofbacterialmetabolicadaptationthroughanintegratedgenomicphenomicapproach AT arturofabiani ecologyofcoldenvironmentsnewinsightsofbacterialmetabolicadaptationthroughanintegratedgenomicphenomicapproach AT silviadecuzzi ecologyofcoldenvironmentsnewinsightsofbacterialmetabolicadaptationthroughanintegratedgenomicphenomicapproach AT donatelladepascale ecologyofcoldenvironmentsnewinsightsofbacterialmetabolicadaptationthroughanintegratedgenomicphenomicapproach AT ermenegildaparrilli ecologyofcoldenvironmentsnewinsightsofbacterialmetabolicadaptationthroughanintegratedgenomicphenomicapproach AT marialuisatutino ecologyofcoldenvironmentsnewinsightsofbacterialmetabolicadaptationthroughanintegratedgenomicphenomicapproach AT elenaperrin ecologyofcoldenvironmentsnewinsightsofbacterialmetabolicadaptationthroughanintegratedgenomicphenomicapproach AT emanuelebosi ecologyofcoldenvironmentsnewinsightsofbacterialmetabolicadaptationthroughanintegratedgenomicphenomicapproach AT marcofondi ecologyofcoldenvironmentsnewinsightsofbacterialmetabolicadaptationthroughanintegratedgenomicphenomicapproach AT angelinalogiudice ecologyofcoldenvironmentsnewinsightsofbacterialmetabolicadaptationthroughanintegratedgenomicphenomicapproach AT renatofani ecologyofcoldenvironmentsnewinsightsofbacterialmetabolicadaptationthroughanintegratedgenomicphenomicapproach |
| _version_ |
1718388785155145728 |