Adult Neural Stem Cells from Midbrain Periventricular Regions Show Limited Neurogenic Potential after Transplantation into the Hippocampal Neurogenic Niche

The regulation of adult neural stem or progenitor cell (aNSC) proliferation and differentiation as an interplay of cell-intrinsic and local environmental cues remains in part unclear, impeding their role in putative regenerative therapies. aNSCs with all major properties of NSCs in vitro have been i...

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Autores principales:	Mareike Fauser, Kai F Loewenbrück, Johannes Rangnick, Moritz D Brandt, Andreas Hermann, Alexander Storch
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	adult neural stem cells adult neurogenesis cell transplantation neurogenic niche dentate gyrus non-neurogenic region Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a43aa66860c7470794ea6699d1e87086
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spelling	oai:doaj.org-article:a43aa66860c7470794ea6699d1e870862021-11-25T17:10:28ZAdult Neural Stem Cells from Midbrain Periventricular Regions Show Limited Neurogenic Potential after Transplantation into the Hippocampal Neurogenic Niche10.3390/cells101130212073-4409https://doaj.org/article/a43aa66860c7470794ea6699d1e870862021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3021https://doaj.org/toc/2073-4409The regulation of adult neural stem or progenitor cell (aNSC) proliferation and differentiation as an interplay of cell-intrinsic and local environmental cues remains in part unclear, impeding their role in putative regenerative therapies. aNSCs with all major properties of NSCs in vitro have been identified in a variety of brain regions beyond the classic neurogenic niches, including the caudal periventricular regions (PVRs) of the midbrain, though active neurogenesis is either limited or merely absent in these regions. To elucidate cell-intrinsic properties of aNSCs from various PVRs, we here examined the proliferation and early differentiation capacity of murine aNSCs from non-neurogenic midbrain PVRs (PVR<sub>MB</sub>) compared to aNSCs from the neurogenic ventricular-subventricular zone (PVR<sub>V-SVZ</sub>) 7 days after transplantation into the permissive pro-neurogenic niche of the dentate gyrus (DG) of the hippocampus in mice. An initial in vitro characterization of the transplants displayed very similar characteristics of both aNSC grafts after in vitro expansion with equal capacities of terminal differentiation into astrocytes and Tuj1<sup>+</sup> neurons. Upon the allogenic transplantation of the respective aNSCs into the DG, PVR<sub>MB</sub> grafts showed a significantly lower graft survival and proliferative capacity compared to PVR<sub>V-SVZ</sub> transplants, whereby the latter are exclusively capable of generating new neurons. Although these differences might be—in part—related to the transplantation procedure and the short-term study design, our data strongly imply important cell-intrinsic differences between aNSCs from neurogenic compared to non-neurogenic PVRs with respect to their neurogenic potential and/or their sensitivity to neurogenic cues.Mareike FauserKai F LoewenbrückJohannes RangnickMoritz D BrandtAndreas HermannAlexander StorchMDPI AGarticleadult neural stem cellsadult neurogenesiscell transplantationneurogenic nichedentate gyrusnon-neurogenic regionBiology (General)QH301-705.5ENCells, Vol 10, Iss 3021, p 3021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adult neural stem cells adult neurogenesis cell transplantation neurogenic niche dentate gyrus non-neurogenic region Biology (General) QH301-705.5
spellingShingle	adult neural stem cells adult neurogenesis cell transplantation neurogenic niche dentate gyrus non-neurogenic region Biology (General) QH301-705.5 Mareike Fauser Kai F Loewenbrück Johannes Rangnick Moritz D Brandt Andreas Hermann Alexander Storch Adult Neural Stem Cells from Midbrain Periventricular Regions Show Limited Neurogenic Potential after Transplantation into the Hippocampal Neurogenic Niche
description	The regulation of adult neural stem or progenitor cell (aNSC) proliferation and differentiation as an interplay of cell-intrinsic and local environmental cues remains in part unclear, impeding their role in putative regenerative therapies. aNSCs with all major properties of NSCs in vitro have been identified in a variety of brain regions beyond the classic neurogenic niches, including the caudal periventricular regions (PVRs) of the midbrain, though active neurogenesis is either limited or merely absent in these regions. To elucidate cell-intrinsic properties of aNSCs from various PVRs, we here examined the proliferation and early differentiation capacity of murine aNSCs from non-neurogenic midbrain PVRs (PVR<sub>MB</sub>) compared to aNSCs from the neurogenic ventricular-subventricular zone (PVR<sub>V-SVZ</sub>) 7 days after transplantation into the permissive pro-neurogenic niche of the dentate gyrus (DG) of the hippocampus in mice. An initial in vitro characterization of the transplants displayed very similar characteristics of both aNSC grafts after in vitro expansion with equal capacities of terminal differentiation into astrocytes and Tuj1<sup>+</sup> neurons. Upon the allogenic transplantation of the respective aNSCs into the DG, PVR<sub>MB</sub> grafts showed a significantly lower graft survival and proliferative capacity compared to PVR<sub>V-SVZ</sub> transplants, whereby the latter are exclusively capable of generating new neurons. Although these differences might be—in part—related to the transplantation procedure and the short-term study design, our data strongly imply important cell-intrinsic differences between aNSCs from neurogenic compared to non-neurogenic PVRs with respect to their neurogenic potential and/or their sensitivity to neurogenic cues.
format	article
author	Mareike Fauser Kai F Loewenbrück Johannes Rangnick Moritz D Brandt Andreas Hermann Alexander Storch
author_facet	Mareike Fauser Kai F Loewenbrück Johannes Rangnick Moritz D Brandt Andreas Hermann Alexander Storch
author_sort	Mareike Fauser
title	Adult Neural Stem Cells from Midbrain Periventricular Regions Show Limited Neurogenic Potential after Transplantation into the Hippocampal Neurogenic Niche
title_short	Adult Neural Stem Cells from Midbrain Periventricular Regions Show Limited Neurogenic Potential after Transplantation into the Hippocampal Neurogenic Niche
title_full	Adult Neural Stem Cells from Midbrain Periventricular Regions Show Limited Neurogenic Potential after Transplantation into the Hippocampal Neurogenic Niche
title_fullStr	Adult Neural Stem Cells from Midbrain Periventricular Regions Show Limited Neurogenic Potential after Transplantation into the Hippocampal Neurogenic Niche
title_full_unstemmed	Adult Neural Stem Cells from Midbrain Periventricular Regions Show Limited Neurogenic Potential after Transplantation into the Hippocampal Neurogenic Niche
title_sort	adult neural stem cells from midbrain periventricular regions show limited neurogenic potential after transplantation into the hippocampal neurogenic niche
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a43aa66860c7470794ea6699d1e87086
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Adult Neural Stem Cells from Midbrain Periventricular Regions Show Limited Neurogenic Potential after Transplantation into the Hippocampal Neurogenic Niche

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