Enhanced penetration into 3D cell culture using two and three layered gold nanoparticles

Christopher G England,1 Thomas Priest,2 Guandong Zhang,2 Xinghua Sun,2 Dhruvinkumar N Patel,2 Lacey R McNally,3,4 Victor van Berkel,4,5 André M Gobin,2 Hermann B Frieboes1,2,41Department of Pharmacology and Toxicology, 2Department of Bioengineering, 3Department of Medicine, 4James Graham...

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Autores principales:	Engl, CG, Priest T, Zhang G, Sun X, Patel DN, McNally LR, van Berkel V, Gobin AM, Frieboes HB
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a444e78fdc46408d9217ba36e88d293a
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spelling	oai:doaj.org-article:a444e78fdc46408d9217ba36e88d293a2021-12-02T02:42:07ZEnhanced penetration into 3D cell culture using two and three layered gold nanoparticles1176-91141178-2013https://doaj.org/article/a444e78fdc46408d9217ba36e88d293a2013-10-01T00:00:00Zhttp://www.dovepress.com/enhanced-penetration-into-3d-cell-culture-using-two-and-three-layered--a14536https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Christopher G England,1 Thomas Priest,2 Guandong Zhang,2 Xinghua Sun,2 Dhruvinkumar N Patel,2 Lacey R McNally,3,4 Victor van Berkel,4,5 André M Gobin,2 Hermann B Frieboes1,2,41Department of Pharmacology and Toxicology, 2Department of Bioengineering, 3Department of Medicine, 4James Graham Brown Cancer Center, 5Department of Surgery, University of Louisville, KY, USAAbstract: Nano-scale particles sized 10–400 nm administered systemically preferentially extravasate from tumor vasculature due to the enhanced permeability and retention effect. Therapeutic success remains elusive, however, because of inhomogeneous particle distribution within tumor tissue. Insufficient tumor vascularization limits particle transport and also results in avascular hypoxic regions with non-proliferating cells, which can regenerate tissue after nanoparticle-delivered cytotoxicity or thermal ablation. Nanoparticle surface modifications provide for increasing tumor targeting and uptake while decreasing immunogenicity and toxicity. Herein, we created novel two layer gold-nanoshell particles coated with alkanethiol and phosphatidylcholine, and three layer nanoshells additionally coated with high-density-lipoprotein. We hypothesize that these particles have enhanced penetration into 3-dimensional cell cultures modeling avascular tissue when compared to standard poly(ethylene glycol) (PEG)-coated nanoshells. Particle uptake and distribution in liver, lung, and pancreatic tumor cell cultures were evaluated using silver-enhancement staining and hyperspectral imaging with dark field microscopy. Two layer nanoshells exhibited significantly higher uptake compared to PEGylated nanoshells. This multilayer formulation may help overcome transport barriers presented by tumor vasculature, and could be further investigated in vivo as a platform for targeted cancer therapies.Keywords: cancer nanotherapy, tumor hypoxia, nanovector transportEnglCGPriest TZhang GSun XPatel DNMcNally LRvan Berkel VGobin AMFrieboes HBDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss Issue 1, Pp 3603-3617 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Engl CG Priest T Zhang G Sun X Patel DN McNally LR van Berkel V Gobin AM Frieboes HB Enhanced penetration into 3D cell culture using two and three layered gold nanoparticles
description	Christopher G England,1 Thomas Priest,2 Guandong Zhang,2 Xinghua Sun,2 Dhruvinkumar N Patel,2 Lacey R McNally,3,4 Victor van Berkel,4,5 André M Gobin,2 Hermann B Frieboes1,2,41Department of Pharmacology and Toxicology, 2Department of Bioengineering, 3Department of Medicine, 4James Graham Brown Cancer Center, 5Department of Surgery, University of Louisville, KY, USAAbstract: Nano-scale particles sized 10–400 nm administered systemically preferentially extravasate from tumor vasculature due to the enhanced permeability and retention effect. Therapeutic success remains elusive, however, because of inhomogeneous particle distribution within tumor tissue. Insufficient tumor vascularization limits particle transport and also results in avascular hypoxic regions with non-proliferating cells, which can regenerate tissue after nanoparticle-delivered cytotoxicity or thermal ablation. Nanoparticle surface modifications provide for increasing tumor targeting and uptake while decreasing immunogenicity and toxicity. Herein, we created novel two layer gold-nanoshell particles coated with alkanethiol and phosphatidylcholine, and three layer nanoshells additionally coated with high-density-lipoprotein. We hypothesize that these particles have enhanced penetration into 3-dimensional cell cultures modeling avascular tissue when compared to standard poly(ethylene glycol) (PEG)-coated nanoshells. Particle uptake and distribution in liver, lung, and pancreatic tumor cell cultures were evaluated using silver-enhancement staining and hyperspectral imaging with dark field microscopy. Two layer nanoshells exhibited significantly higher uptake compared to PEGylated nanoshells. This multilayer formulation may help overcome transport barriers presented by tumor vasculature, and could be further investigated in vivo as a platform for targeted cancer therapies.Keywords: cancer nanotherapy, tumor hypoxia, nanovector transport
format	article
author	Engl CG Priest T Zhang G Sun X Patel DN McNally LR van Berkel V Gobin AM Frieboes HB
author_facet	Engl CG Priest T Zhang G Sun X Patel DN McNally LR van Berkel V Gobin AM Frieboes HB
author_sort	Engl
title	Enhanced penetration into 3D cell culture using two and three layered gold nanoparticles
title_short	Enhanced penetration into 3D cell culture using two and three layered gold nanoparticles
title_full	Enhanced penetration into 3D cell culture using two and three layered gold nanoparticles
title_fullStr	Enhanced penetration into 3D cell culture using two and three layered gold nanoparticles
title_full_unstemmed	Enhanced penetration into 3D cell culture using two and three layered gold nanoparticles
title_sort	enhanced penetration into 3d cell culture using two and three layered gold nanoparticles
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/a444e78fdc46408d9217ba36e88d293a
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Enhanced penetration into 3D cell culture using two and three layered gold nanoparticles

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