A beta-glucosidase of an insect herbivore determines both toxicity and deterrence of a dandelion defense metabolite
Gut enzymes can metabolize plant defense compounds and thereby affect the growth and fitness of insect herbivores. Whether these enzymes also influence feeding preference is largely unknown. We studied the metabolization of taraxinic acid β-D-glucopyranosyl ester (TA-G), a sesquiterpene lactone of t...
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eLife Sciences Publications Ltd
2021
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oai:doaj.org-article:a447ed9d0bc24028bd2ac0f7ba6576702021-11-25T12:33:45ZA beta-glucosidase of an insect herbivore determines both toxicity and deterrence of a dandelion defense metabolite10.7554/eLife.686422050-084Xe68642https://doaj.org/article/a447ed9d0bc24028bd2ac0f7ba6576702021-10-01T00:00:00Zhttps://elifesciences.org/articles/68642https://doaj.org/toc/2050-084XGut enzymes can metabolize plant defense compounds and thereby affect the growth and fitness of insect herbivores. Whether these enzymes also influence feeding preference is largely unknown. We studied the metabolization of taraxinic acid β-D-glucopyranosyl ester (TA-G), a sesquiterpene lactone of the common dandelion (Taraxacum officinale) that deters its major root herbivore, the common cockchafer larva (Melolontha melolontha). We have demonstrated that TA-G is rapidly deglucosylated and conjugated to glutathione in the insect gut. A broad-spectrum M. melolontha β-glucosidase, Mm_bGlc17, is sufficient and necessary for TA-G deglucosylation. Using cross-species RNA interference, we have shown that Mm_bGlc17 reduces TA-G toxicity. Furthermore, Mm_bGlc17 is required for the preference of M. melolontha larvae for TA-G-deficient plants. Thus, herbivore metabolism modulates both the toxicity and deterrence of a plant defense compound. Our work illustrates the multifaceted roles of insect digestive enzymes as mediators of plant-herbivore interactions.Meret HuberThomas RoderSandra IrmischAlexander RiedelSaskia GablenzJulia FrickePeter RahfeldMichael ReicheltChristian PaetzNicole LiechtiLingfei HuZoe BontYe MengWei HuangChristelle AM RobertJonathan GershenzonMatthias ErbeLife Sciences Publications LtdarticleMelolontha melolonthaTaraxacum officinaleplant defenseroot herbivoresesquiterpene lactoneβ-glucosidaseMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021) |
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| collection |
DOAJ |
| language |
EN |
| topic |
Melolontha melolontha Taraxacum officinale plant defense root herbivore sesquiterpene lactone β-glucosidase Medicine R Science Q Biology (General) QH301-705.5 |
| spellingShingle |
Melolontha melolontha Taraxacum officinale plant defense root herbivore sesquiterpene lactone β-glucosidase Medicine R Science Q Biology (General) QH301-705.5 Meret Huber Thomas Roder Sandra Irmisch Alexander Riedel Saskia Gablenz Julia Fricke Peter Rahfeld Michael Reichelt Christian Paetz Nicole Liechti Lingfei Hu Zoe Bont Ye Meng Wei Huang Christelle AM Robert Jonathan Gershenzon Matthias Erb A beta-glucosidase of an insect herbivore determines both toxicity and deterrence of a dandelion defense metabolite |
| description |
Gut enzymes can metabolize plant defense compounds and thereby affect the growth and fitness of insect herbivores. Whether these enzymes also influence feeding preference is largely unknown. We studied the metabolization of taraxinic acid β-D-glucopyranosyl ester (TA-G), a sesquiterpene lactone of the common dandelion (Taraxacum officinale) that deters its major root herbivore, the common cockchafer larva (Melolontha melolontha). We have demonstrated that TA-G is rapidly deglucosylated and conjugated to glutathione in the insect gut. A broad-spectrum M. melolontha β-glucosidase, Mm_bGlc17, is sufficient and necessary for TA-G deglucosylation. Using cross-species RNA interference, we have shown that Mm_bGlc17 reduces TA-G toxicity. Furthermore, Mm_bGlc17 is required for the preference of M. melolontha larvae for TA-G-deficient plants. Thus, herbivore metabolism modulates both the toxicity and deterrence of a plant defense compound. Our work illustrates the multifaceted roles of insect digestive enzymes as mediators of plant-herbivore interactions. |
| format |
article |
| author |
Meret Huber Thomas Roder Sandra Irmisch Alexander Riedel Saskia Gablenz Julia Fricke Peter Rahfeld Michael Reichelt Christian Paetz Nicole Liechti Lingfei Hu Zoe Bont Ye Meng Wei Huang Christelle AM Robert Jonathan Gershenzon Matthias Erb |
| author_facet |
Meret Huber Thomas Roder Sandra Irmisch Alexander Riedel Saskia Gablenz Julia Fricke Peter Rahfeld Michael Reichelt Christian Paetz Nicole Liechti Lingfei Hu Zoe Bont Ye Meng Wei Huang Christelle AM Robert Jonathan Gershenzon Matthias Erb |
| author_sort |
Meret Huber |
| title |
A beta-glucosidase of an insect herbivore determines both toxicity and deterrence of a dandelion defense metabolite |
| title_short |
A beta-glucosidase of an insect herbivore determines both toxicity and deterrence of a dandelion defense metabolite |
| title_full |
A beta-glucosidase of an insect herbivore determines both toxicity and deterrence of a dandelion defense metabolite |
| title_fullStr |
A beta-glucosidase of an insect herbivore determines both toxicity and deterrence of a dandelion defense metabolite |
| title_full_unstemmed |
A beta-glucosidase of an insect herbivore determines both toxicity and deterrence of a dandelion defense metabolite |
| title_sort |
beta-glucosidase of an insect herbivore determines both toxicity and deterrence of a dandelion defense metabolite |
| publisher |
eLife Sciences Publications Ltd |
| publishDate |
2021 |
| url |
https://doaj.org/article/a447ed9d0bc24028bd2ac0f7ba657670 |
| work_keys_str_mv |
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| _version_ |
1718413516939984896 |