Impact of Storage Conditions on Equine Fecal Inoculum for Estimating In Vitro Digestibility

This study evaluated the effect of storage conditions of equine fecal material on the viability of microbial inoculum used for in vitro equine digestibility trials. Pooled fecal material from three mature Quarter Horse geldings was stored at 39 °C anaerobically for 15 min (control), while aerobic sa...

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Autores principales:	Delaney O’Donnell, Lacy Sukovaty, Gary Webb
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	equine digestibility microbial inoculum in vitro inoculum storage Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/a44916ce7b4d4bec86d7b629b541acd3
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spelling	oai:doaj.org-article:a44916ce7b4d4bec86d7b629b541acd32021-11-25T16:18:47ZImpact of Storage Conditions on Equine Fecal Inoculum for Estimating In Vitro Digestibility10.3390/ani111131952076-2615https://doaj.org/article/a44916ce7b4d4bec86d7b629b541acd32021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3195https://doaj.org/toc/2076-2615This study evaluated the effect of storage conditions of equine fecal material on the viability of microbial inoculum used for in vitro equine digestibility trials. Pooled fecal material from three mature Quarter Horse geldings was stored at 39 °C anaerobically for 15 min (control), while aerobic samples were stored at 22 °C for 6 h (SC1), 3 °C for 6 h (SC2), and −18 °C for 24 h (SC3). Following storage, the feces were utilized to prepare microbial inoculum for the digestion of six different forages using the Daisy II Incubator. After incubation, DM, NDF, and ADF compositions were determined and used to calculate DMD, NDFD, and ADFD. Analysis using the OLS regression model for differences in DMD, NDFD, and ADFD across the storage conditions found significant interactions between the forage sample and the storage condition (<i>p</i> < 0.05). The results between the control and SC1, SC2, and SC3 were not different (<i>p</i> < 0.8). Fecal material stored aerobically for six hours at 22 °C provided similar digestibility estimates compared to the control, while DMD decreased by 3.86% in SC2 and by 4.08% in SC3.Delaney O’DonnellLacy SukovatyGary WebbMDPI AGarticleequinedigestibilitymicrobial inoculumin vitroinoculum storageVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3195, p 3195 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	equine digestibility microbial inoculum in vitro inoculum storage Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	equine digestibility microbial inoculum in vitro inoculum storage Veterinary medicine SF600-1100 Zoology QL1-991 Delaney O’Donnell Lacy Sukovaty Gary Webb Impact of Storage Conditions on Equine Fecal Inoculum for Estimating In Vitro Digestibility
description	This study evaluated the effect of storage conditions of equine fecal material on the viability of microbial inoculum used for in vitro equine digestibility trials. Pooled fecal material from three mature Quarter Horse geldings was stored at 39 °C anaerobically for 15 min (control), while aerobic samples were stored at 22 °C for 6 h (SC1), 3 °C for 6 h (SC2), and −18 °C for 24 h (SC3). Following storage, the feces were utilized to prepare microbial inoculum for the digestion of six different forages using the Daisy II Incubator. After incubation, DM, NDF, and ADF compositions were determined and used to calculate DMD, NDFD, and ADFD. Analysis using the OLS regression model for differences in DMD, NDFD, and ADFD across the storage conditions found significant interactions between the forage sample and the storage condition (<i>p</i> < 0.05). The results between the control and SC1, SC2, and SC3 were not different (<i>p</i> < 0.8). Fecal material stored aerobically for six hours at 22 °C provided similar digestibility estimates compared to the control, while DMD decreased by 3.86% in SC2 and by 4.08% in SC3.
format	article
author	Delaney O’Donnell Lacy Sukovaty Gary Webb
author_facet	Delaney O’Donnell Lacy Sukovaty Gary Webb
author_sort	Delaney O’Donnell
title	Impact of Storage Conditions on Equine Fecal Inoculum for Estimating In Vitro Digestibility
title_short	Impact of Storage Conditions on Equine Fecal Inoculum for Estimating In Vitro Digestibility
title_full	Impact of Storage Conditions on Equine Fecal Inoculum for Estimating In Vitro Digestibility
title_fullStr	Impact of Storage Conditions on Equine Fecal Inoculum for Estimating In Vitro Digestibility
title_full_unstemmed	Impact of Storage Conditions on Equine Fecal Inoculum for Estimating In Vitro Digestibility
title_sort	impact of storage conditions on equine fecal inoculum for estimating in vitro digestibility
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a44916ce7b4d4bec86d7b629b541acd3
work_keys_str_mv	AT delaneyodonnell impactofstorageconditionsonequinefecalinoculumforestimatinginvitrodigestibility AT lacysukovaty impactofstorageconditionsonequinefecalinoculumforestimatinginvitrodigestibility AT garywebb impactofstorageconditionsonequinefecalinoculumforestimatinginvitrodigestibility
_version_	1718413273394577408

Impact of Storage Conditions on Equine Fecal Inoculum for Estimating In Vitro Digestibility

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