Genomic Analysis of Multidrug-Resistant Mycobacterium tuberculosis Strains From Patients in Kazakhstan

Tuberculosis (TB) is an infectious disease that remains an essential public health problem in many countries. Despite decreasing numbers of new cases worldwide, the incidence of antibiotic-resistant forms (multidrug resistant and extensively drug-resistant) of TB is increasing. Next-generation seque...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Asset Daniyarov, Askhat Molkenov, Saule Rakhimova, Ainur Akhmetova, Dauren Yerezhepov, Lyailya Chingissova, Venera Bismilda, Bekzat Toksanbayeva, Anar Rakisheva, Ainur Akilzhanova, Ulan Kozhamkulov, Ulykbek Kairov
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	tuberculosis genomic analysis whole genome genetic variants Mycobacterium tuberculosis multidrug resistant TB Genetics QH426-470
Acceso en línea:	https://doaj.org/article/a44e2d98233940709c585c2dff2e993d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a44e2d98233940709c585c2dff2e993d
record_format	dspace
spelling	oai:doaj.org-article:a44e2d98233940709c585c2dff2e993d2021-11-09T05:10:10ZGenomic Analysis of Multidrug-Resistant Mycobacterium tuberculosis Strains From Patients in Kazakhstan1664-802110.3389/fgene.2021.683515https://doaj.org/article/a44e2d98233940709c585c2dff2e993d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.683515/fullhttps://doaj.org/toc/1664-8021Tuberculosis (TB) is an infectious disease that remains an essential public health problem in many countries. Despite decreasing numbers of new cases worldwide, the incidence of antibiotic-resistant forms (multidrug resistant and extensively drug-resistant) of TB is increasing. Next-generation sequencing technologies provide a high-throughput approach to identify known and novel potential genetic variants that are associated with drug resistance in Mycobacterium tuberculosis (Mtb). There are limited reports and data related to whole-genome characteristics of drug-resistant Mtb strains circulating in Kazakhstan. Here, we report whole-genome sequencing and analysis results of eight multidrug-resistant strains collected from TB patients in Kazakhstan. Genotyping and validation of all strains by MIRU-VNTR and spoligotyping methodologies revealed that these strains belong to the Beijing family. The spectrum of specific and potentially novel genomic variants (single-nucleotide polymorphisms, insertions, and deletions) related to drug resistance was identified and annotated. ResFinder, CARD, and CASTB antibiotic resistance databases were used for the characterization of genetic variants in genes associated with drug resistance. Our results provide reference data and genomic profiles of multidrug-resistant isolates for further comparative studies and investigations of genetic patterns in drug-resistant Mtb strains.Asset DaniyarovAskhat MolkenovSaule RakhimovaAinur AkhmetovaDauren YerezhepovLyailya ChingissovaVenera BismildaBekzat ToksanbayevaAnar RakishevaAinur AkilzhanovaUlan KozhamkulovUlykbek KairovFrontiers Media S.A.articletuberculosisgenomic analysiswhole genomegenetic variantsMycobacterium tuberculosismultidrug resistant TBGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	tuberculosis genomic analysis whole genome genetic variants Mycobacterium tuberculosis multidrug resistant TB Genetics QH426-470
spellingShingle	tuberculosis genomic analysis whole genome genetic variants Mycobacterium tuberculosis multidrug resistant TB Genetics QH426-470 Asset Daniyarov Askhat Molkenov Saule Rakhimova Ainur Akhmetova Dauren Yerezhepov Lyailya Chingissova Venera Bismilda Bekzat Toksanbayeva Anar Rakisheva Ainur Akilzhanova Ulan Kozhamkulov Ulykbek Kairov Genomic Analysis of Multidrug-Resistant Mycobacterium tuberculosis Strains From Patients in Kazakhstan
description	Tuberculosis (TB) is an infectious disease that remains an essential public health problem in many countries. Despite decreasing numbers of new cases worldwide, the incidence of antibiotic-resistant forms (multidrug resistant and extensively drug-resistant) of TB is increasing. Next-generation sequencing technologies provide a high-throughput approach to identify known and novel potential genetic variants that are associated with drug resistance in Mycobacterium tuberculosis (Mtb). There are limited reports and data related to whole-genome characteristics of drug-resistant Mtb strains circulating in Kazakhstan. Here, we report whole-genome sequencing and analysis results of eight multidrug-resistant strains collected from TB patients in Kazakhstan. Genotyping and validation of all strains by MIRU-VNTR and spoligotyping methodologies revealed that these strains belong to the Beijing family. The spectrum of specific and potentially novel genomic variants (single-nucleotide polymorphisms, insertions, and deletions) related to drug resistance was identified and annotated. ResFinder, CARD, and CASTB antibiotic resistance databases were used for the characterization of genetic variants in genes associated with drug resistance. Our results provide reference data and genomic profiles of multidrug-resistant isolates for further comparative studies and investigations of genetic patterns in drug-resistant Mtb strains.
format	article
author	Asset Daniyarov Askhat Molkenov Saule Rakhimova Ainur Akhmetova Dauren Yerezhepov Lyailya Chingissova Venera Bismilda Bekzat Toksanbayeva Anar Rakisheva Ainur Akilzhanova Ulan Kozhamkulov Ulykbek Kairov
author_facet	Asset Daniyarov Askhat Molkenov Saule Rakhimova Ainur Akhmetova Dauren Yerezhepov Lyailya Chingissova Venera Bismilda Bekzat Toksanbayeva Anar Rakisheva Ainur Akilzhanova Ulan Kozhamkulov Ulykbek Kairov
author_sort	Asset Daniyarov
title	Genomic Analysis of Multidrug-Resistant Mycobacterium tuberculosis Strains From Patients in Kazakhstan
title_short	Genomic Analysis of Multidrug-Resistant Mycobacterium tuberculosis Strains From Patients in Kazakhstan
title_full	Genomic Analysis of Multidrug-Resistant Mycobacterium tuberculosis Strains From Patients in Kazakhstan
title_fullStr	Genomic Analysis of Multidrug-Resistant Mycobacterium tuberculosis Strains From Patients in Kazakhstan
title_full_unstemmed	Genomic Analysis of Multidrug-Resistant Mycobacterium tuberculosis Strains From Patients in Kazakhstan
title_sort	genomic analysis of multidrug-resistant mycobacterium tuberculosis strains from patients in kazakhstan
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/a44e2d98233940709c585c2dff2e993d
work_keys_str_mv	AT assetdaniyarov genomicanalysisofmultidrugresistantmycobacteriumtuberculosisstrainsfrompatientsinkazakhstan AT askhatmolkenov genomicanalysisofmultidrugresistantmycobacteriumtuberculosisstrainsfrompatientsinkazakhstan AT saulerakhimova genomicanalysisofmultidrugresistantmycobacteriumtuberculosisstrainsfrompatientsinkazakhstan AT ainurakhmetova genomicanalysisofmultidrugresistantmycobacteriumtuberculosisstrainsfrompatientsinkazakhstan AT daurenyerezhepov genomicanalysisofmultidrugresistantmycobacteriumtuberculosisstrainsfrompatientsinkazakhstan AT lyailyachingissova genomicanalysisofmultidrugresistantmycobacteriumtuberculosisstrainsfrompatientsinkazakhstan AT venerabismilda genomicanalysisofmultidrugresistantmycobacteriumtuberculosisstrainsfrompatientsinkazakhstan AT bekzattoksanbayeva genomicanalysisofmultidrugresistantmycobacteriumtuberculosisstrainsfrompatientsinkazakhstan AT anarrakisheva genomicanalysisofmultidrugresistantmycobacteriumtuberculosisstrainsfrompatientsinkazakhstan AT ainurakilzhanova genomicanalysisofmultidrugresistantmycobacteriumtuberculosisstrainsfrompatientsinkazakhstan AT ulankozhamkulov genomicanalysisofmultidrugresistantmycobacteriumtuberculosisstrainsfrompatientsinkazakhstan AT ulykbekkairov genomicanalysisofmultidrugresistantmycobacteriumtuberculosisstrainsfrompatientsinkazakhstan
_version_	1718441322029776896

Genomic Analysis of Multidrug-Resistant Mycobacterium tuberculosis Strains From Patients in Kazakhstan

Ejemplares similares