The Lyme disease agent co-opts adiponectin receptor-mediated signaling in its arthropod vector
Adiponectin-mediated pathways contribute to mammalian homeostasis; however, little is known about adiponectin and adiponectin receptor signaling in arthropods. In this study, we demonstrate that Ixodes scapularis ticks have an adiponectin receptor-like protein (ISARL) but lack adiponectin, suggestin...
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eLife Sciences Publications Ltd
2021
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oai:doaj.org-article:a44fd9503ecd448a8e723336d1c6c5fc2021-12-02T11:50:11ZThe Lyme disease agent co-opts adiponectin receptor-mediated signaling in its arthropod vector10.7554/eLife.725682050-084Xe72568https://doaj.org/article/a44fd9503ecd448a8e723336d1c6c5fc2021-11-01T00:00:00Zhttps://elifesciences.org/articles/72568https://doaj.org/toc/2050-084XAdiponectin-mediated pathways contribute to mammalian homeostasis; however, little is known about adiponectin and adiponectin receptor signaling in arthropods. In this study, we demonstrate that Ixodes scapularis ticks have an adiponectin receptor-like protein (ISARL) but lack adiponectin, suggesting activation by alternative pathways. ISARL expression is significantly upregulated in the tick gut after Borrelia burgdorferi infection, suggesting that ISARL signaling may be co-opted by the Lyme disease agent. Consistent with this, RNA interference (RNAi)-mediated silencing of ISARL significantly reduced the B. burgdorferi burden in the tick. RNA-seq-based transcriptomics and RNAi assays demonstrate that ISARL-mediated phospholipid metabolism by phosphatidylserine synthase I is associated with B. burgdorferi survival. Furthermore, the tick complement C1q-like protein 3 interacts with ISARL, and B. burgdorferi facilitates this process. This study identifies a new tick metabolic pathway that is connected to the life cycle of the Lyme disease spirochete.Xiaotian TangYongguo CaoGunjan AroraJesse HwangAndaleeb SajidCourtney L BrownSameet MehtaAlejandro Marín-LópezYu-Min ChuangMing-Jie WuHongwei MaUtpal PalSukanya NarasimhanErol FikrigeLife Sciences Publications Ltdarticleadiponectin receptorIxodes scapularisBorrelia burgdorferiMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021) |
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DOAJ |
| collection |
DOAJ |
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| topic |
adiponectin receptor Ixodes scapularis Borrelia burgdorferi Medicine R Science Q Biology (General) QH301-705.5 |
| spellingShingle |
adiponectin receptor Ixodes scapularis Borrelia burgdorferi Medicine R Science Q Biology (General) QH301-705.5 Xiaotian Tang Yongguo Cao Gunjan Arora Jesse Hwang Andaleeb Sajid Courtney L Brown Sameet Mehta Alejandro Marín-López Yu-Min Chuang Ming-Jie Wu Hongwei Ma Utpal Pal Sukanya Narasimhan Erol Fikrig The Lyme disease agent co-opts adiponectin receptor-mediated signaling in its arthropod vector |
| description |
Adiponectin-mediated pathways contribute to mammalian homeostasis; however, little is known about adiponectin and adiponectin receptor signaling in arthropods. In this study, we demonstrate that Ixodes scapularis ticks have an adiponectin receptor-like protein (ISARL) but lack adiponectin, suggesting activation by alternative pathways. ISARL expression is significantly upregulated in the tick gut after Borrelia burgdorferi infection, suggesting that ISARL signaling may be co-opted by the Lyme disease agent. Consistent with this, RNA interference (RNAi)-mediated silencing of ISARL significantly reduced the B. burgdorferi burden in the tick. RNA-seq-based transcriptomics and RNAi assays demonstrate that ISARL-mediated phospholipid metabolism by phosphatidylserine synthase I is associated with B. burgdorferi survival. Furthermore, the tick complement C1q-like protein 3 interacts with ISARL, and B. burgdorferi facilitates this process. This study identifies a new tick metabolic pathway that is connected to the life cycle of the Lyme disease spirochete. |
| format |
article |
| author |
Xiaotian Tang Yongguo Cao Gunjan Arora Jesse Hwang Andaleeb Sajid Courtney L Brown Sameet Mehta Alejandro Marín-López Yu-Min Chuang Ming-Jie Wu Hongwei Ma Utpal Pal Sukanya Narasimhan Erol Fikrig |
| author_facet |
Xiaotian Tang Yongguo Cao Gunjan Arora Jesse Hwang Andaleeb Sajid Courtney L Brown Sameet Mehta Alejandro Marín-López Yu-Min Chuang Ming-Jie Wu Hongwei Ma Utpal Pal Sukanya Narasimhan Erol Fikrig |
| author_sort |
Xiaotian Tang |
| title |
The Lyme disease agent co-opts adiponectin receptor-mediated signaling in its arthropod vector |
| title_short |
The Lyme disease agent co-opts adiponectin receptor-mediated signaling in its arthropod vector |
| title_full |
The Lyme disease agent co-opts adiponectin receptor-mediated signaling in its arthropod vector |
| title_fullStr |
The Lyme disease agent co-opts adiponectin receptor-mediated signaling in its arthropod vector |
| title_full_unstemmed |
The Lyme disease agent co-opts adiponectin receptor-mediated signaling in its arthropod vector |
| title_sort |
lyme disease agent co-opts adiponectin receptor-mediated signaling in its arthropod vector |
| publisher |
eLife Sciences Publications Ltd |
| publishDate |
2021 |
| url |
https://doaj.org/article/a44fd9503ecd448a8e723336d1c6c5fc |
| work_keys_str_mv |
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