Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET

Abstract Synthetic vascular smooth muscle cells (VSMCs) play important roles in atherosclerosis, in-stent restenosis, and transplant vasculopathy. We investigated the synthetic activity of VSMCs in the atherosclerotic carotid artery using 18F-fluorodeoxyglucose (FDG) positron emission tomography (PE...

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Autores principales:	Kisoo Pahk, Chanmin Joung, Se-Mi Jung, Hwa Young Song, Ji Yong Park, Jung Woo Byun, Yun-Sang Lee, Jin Chul Paeng, Chunsook Kim, Sungeun Kim, Won-Ki Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a44ff81d29b34e39887f2315406f4991
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spelling	oai:doaj.org-article:a44ff81d29b34e39887f2315406f49912021-12-02T16:08:21ZVisualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET10.1038/s41598-017-07073-32045-2322https://doaj.org/article/a44ff81d29b34e39887f2315406f49912017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07073-3https://doaj.org/toc/2045-2322Abstract Synthetic vascular smooth muscle cells (VSMCs) play important roles in atherosclerosis, in-stent restenosis, and transplant vasculopathy. We investigated the synthetic activity of VSMCs in the atherosclerotic carotid artery using 18F-fluorodeoxyglucose (FDG) positron emission tomography (PET). Atherosclerosis was induced in rats by partial ligation of the right carotid artery coupled with an atherogenic diet and vitamin D injections (2 consecutive days, 600,000 IU/day). One month later, rats were imaged by F-18 FDG PET. The atherosclerotic right carotid arteries showed prominent luminal narrowing with neointimal hyperplasia. The regions with neointimal hyperplasia were composed of α-smooth muscle actin-positive cells with decreased expression of smooth muscle myosin heavy chain. Surrogate markers of synthetic VSMCs such as collagen type III, cyclophilin A, and matrix metallopeptidase-9 were increased in neointima region. However, neither macrophages nor neutrophils were observed in regions with neointimal hyperplasia. F-18 FDG PET imaging and autoradiography showed elevated FDG uptake into the atherosclerotic carotid artery. The inner vessel layer showed higher tracer uptake than the outer layer. Consistently, the expression of glucose transporter 1 was highly increased in neointima. The present results indicate that F-18 FDG PET may be a useful tool for evaluating synthetic activities of VSMCs in vascular remodeling disorders.Kisoo PahkChanmin JoungSe-Mi JungHwa Young SongJi Yong ParkJung Woo ByunYun-Sang LeeJin Chul PaengChunsook KimSungeun KimWon-Ki KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kisoo Pahk Chanmin Joung Se-Mi Jung Hwa Young Song Ji Yong Park Jung Woo Byun Yun-Sang Lee Jin Chul Paeng Chunsook Kim Sungeun Kim Won-Ki Kim Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET
description	Abstract Synthetic vascular smooth muscle cells (VSMCs) play important roles in atherosclerosis, in-stent restenosis, and transplant vasculopathy. We investigated the synthetic activity of VSMCs in the atherosclerotic carotid artery using 18F-fluorodeoxyglucose (FDG) positron emission tomography (PET). Atherosclerosis was induced in rats by partial ligation of the right carotid artery coupled with an atherogenic diet and vitamin D injections (2 consecutive days, 600,000 IU/day). One month later, rats were imaged by F-18 FDG PET. The atherosclerotic right carotid arteries showed prominent luminal narrowing with neointimal hyperplasia. The regions with neointimal hyperplasia were composed of α-smooth muscle actin-positive cells with decreased expression of smooth muscle myosin heavy chain. Surrogate markers of synthetic VSMCs such as collagen type III, cyclophilin A, and matrix metallopeptidase-9 were increased in neointima region. However, neither macrophages nor neutrophils were observed in regions with neointimal hyperplasia. F-18 FDG PET imaging and autoradiography showed elevated FDG uptake into the atherosclerotic carotid artery. The inner vessel layer showed higher tracer uptake than the outer layer. Consistently, the expression of glucose transporter 1 was highly increased in neointima. The present results indicate that F-18 FDG PET may be a useful tool for evaluating synthetic activities of VSMCs in vascular remodeling disorders.
format	article
author	Kisoo Pahk Chanmin Joung Se-Mi Jung Hwa Young Song Ji Yong Park Jung Woo Byun Yun-Sang Lee Jin Chul Paeng Chunsook Kim Sungeun Kim Won-Ki Kim
author_facet	Kisoo Pahk Chanmin Joung Se-Mi Jung Hwa Young Song Ji Yong Park Jung Woo Byun Yun-Sang Lee Jin Chul Paeng Chunsook Kim Sungeun Kim Won-Ki Kim
author_sort	Kisoo Pahk
title	Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET
title_short	Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET
title_full	Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET
title_fullStr	Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET
title_full_unstemmed	Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET
title_sort	visualization of synthetic vascular smooth muscle cells in atherosclerotic carotid rat arteries by f-18 fdg pet
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a44ff81d29b34e39887f2315406f4991
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Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET

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