Preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering

Preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering

YueLong Wang,1,2,* Gang Guo,1,* HaiFeng Chen,2 Xiang Gao,1 RangRang Fan,1 DongMei Zhang,1 LiangXue Zhou2 1State Key Laboratory of Biotherapy and Cancer Center, 2Department of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu, People's Republic of Chin...

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Autores principales: Wang YL, Guo G, Chen HF, Gao X, Fan RR, Zhang DM, Zhou LX
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2014
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/a451d20e36a84225ba884d63829ecc63
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spelling oai:doaj.org-article:a451d20e36a84225ba884d63829ecc632021-12-02T02:42:21ZPreparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering1178-2013https://doaj.org/article/a451d20e36a84225ba884d63829ecc632014-04-01T00:00:00Zhttp://www.dovepress.com/preparation-and-characterization-of-polylactidepolyepsilon-caprolacton-a16510https://doaj.org/toc/1178-2013 YueLong Wang,1,2,* Gang Guo,1,* HaiFeng Chen,2 Xiang Gao,1 RangRang Fan,1 DongMei Zhang,1 LiangXue Zhou2 1State Key Laboratory of Biotherapy and Cancer Center, 2Department of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu, People's Republic of China *These authors contributed equally to this paper Abstract: The aim of this study was to develop a kind of osteogenic biodegradable composite graft consisting of human placenta-derived mesenchymal stem cell (hPMSC) material for site-specific repair of bone defects and attenuation of clinical symptoms. The novel nano- to micro-structured biodegradable hybrid fibers were prepared by electrospinning. The characteristics of the hybrid membranes were investigated by a range of methods, including Fourier transform infrared spectroscopy, X-ray diffraction, and differential scanning calorimetry. Morphological study with scanning electron microscopy showed that the average fiber diameter and the number of nanoscale pores on each individual fiber surface decreased with increasing concentration of poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCEC). The prepared polylactide (PLA)/PCEC fibrous membranes favored hPMSC attachment and proliferation by providing an interconnected, porous, three-dimensional mimicked extracellular environment. What is more, hPMSCs cultured on the electrospun hybrid PLA/PCEC fibrous scaffolds could be effectively differentiated into bone-associated cells by positive alizarin red staining. Given the good cellular response and excellent osteogenic potential in vitro, the electrospun PLA/PCEC fibrous scaffolds could be one of the most promising candidates for bone tissue engineering. Keywords: electrospinning, PLA, PCEC, hPMSCs, bone tissue engineeringWang YLGuo GChen HFGao XFan RRZhang DMZhou LXDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 1991-2003 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Wang YL
Guo G
Chen HF
Gao X
Fan RR
Zhang DM
Zhou LX
Preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering
description YueLong Wang,1,2,* Gang Guo,1,* HaiFeng Chen,2 Xiang Gao,1 RangRang Fan,1 DongMei Zhang,1 LiangXue Zhou2 1State Key Laboratory of Biotherapy and Cancer Center, 2Department of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu, People's Republic of China *These authors contributed equally to this paper Abstract: The aim of this study was to develop a kind of osteogenic biodegradable composite graft consisting of human placenta-derived mesenchymal stem cell (hPMSC) material for site-specific repair of bone defects and attenuation of clinical symptoms. The novel nano- to micro-structured biodegradable hybrid fibers were prepared by electrospinning. The characteristics of the hybrid membranes were investigated by a range of methods, including Fourier transform infrared spectroscopy, X-ray diffraction, and differential scanning calorimetry. Morphological study with scanning electron microscopy showed that the average fiber diameter and the number of nanoscale pores on each individual fiber surface decreased with increasing concentration of poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCEC). The prepared polylactide (PLA)/PCEC fibrous membranes favored hPMSC attachment and proliferation by providing an interconnected, porous, three-dimensional mimicked extracellular environment. What is more, hPMSCs cultured on the electrospun hybrid PLA/PCEC fibrous scaffolds could be effectively differentiated into bone-associated cells by positive alizarin red staining. Given the good cellular response and excellent osteogenic potential in vitro, the electrospun PLA/PCEC fibrous scaffolds could be one of the most promising candidates for bone tissue engineering. Keywords: electrospinning, PLA, PCEC, hPMSCs, bone tissue engineering
format article
author Wang YL
Guo G
Chen HF
Gao X
Fan RR
Zhang DM
Zhou LX
author_facet Wang YL
Guo G
Chen HF
Gao X
Fan RR
Zhang DM
Zhou LX
author_sort Wang YL
title Preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering
title_short Preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering
title_full Preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering
title_fullStr Preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering
title_full_unstemmed Preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering
title_sort preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering
publisher Dove Medical Press
publishDate 2014
url https://doaj.org/article/a451d20e36a84225ba884d63829ecc63
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