Relative gene expression, micronuclei formation, and ultrastructure alterations induced by heavy metal contamination in Pimelia latreillei (Coleoptera: Tenebrionidae) in an urban-industrial area of Alexandria, Egypt.

The present research aims to evaluate the impact of industrial processes and anthropogenic activities on the beetle Pimelia latreillei inhabiting the polluted site at Zawya Abd El- Qader, Alexandria, Egypt. Beetles were collected from the vicinity of five factories. The genotoxic effects of environm...

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Autores principales:	Lamia M El-Samad, Saeed El-Ashram, Dalia A Kheirallah, Karolin K Abdul-Aziz, Noura A Toto, El Hassan M Mokhamer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a459ab70decf4e63bd87edd3e112a21d
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spelling	oai:doaj.org-article:a459ab70decf4e63bd87edd3e112a21d2021-12-02T20:10:12ZRelative gene expression, micronuclei formation, and ultrastructure alterations induced by heavy metal contamination in Pimelia latreillei (Coleoptera: Tenebrionidae) in an urban-industrial area of Alexandria, Egypt.1932-620310.1371/journal.pone.0253238https://doaj.org/article/a459ab70decf4e63bd87edd3e112a21d2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253238https://doaj.org/toc/1932-6203The present research aims to evaluate the impact of industrial processes and anthropogenic activities on the beetle Pimelia latreillei inhabiting the polluted site at Zawya Abd El- Qader, Alexandria, Egypt. Beetles were collected from the vicinity of five factories. The genotoxic effects of environmental exposures to industrial heavy metals were monitored using a broad range of assays, including energy-dispersive X ray microanalysis and X-ray diffraction (SEM and EDX)), qRT-PCR gene expression assay, micronuclei formation, and transmission electron microscope (TEM). Energy dispersive X-ray microanalysis for the soil and testicular tissues of beetles collected from the polluted site revealed a higher percentage of heavy metals than the beetles collected from the reference site (Sidi Kirier, Alexandria, Egypt). To analyze/monitor genotoxicity in P. latreillei sampled from the polluted site, the transcription levels of levels of heat shock proteins (Hsps) and accessory gland seminal fluid protein (AcPC01) in testicular tissues were recorded. The incidence of micronuclei (MN) formation in the testicular cells was also observed. Quantitative RT-PCR (RT-qPCR) analysis was carried out to detect the changes in the gene expression of the aforementioned proteins. Genes encoding heat shock proteins (Hsp60, Hsp70, and Hsp90) were significantly overexpressed (> 2-fold) in specimens sampled from the polluted site; however, AcPC01 gene expression was under-expressed (<1.5-folds). The incidence of MN was significantly increased in specimens sampled from the polluted site. Ultrastructure anomalies (nuclear and cytoplasmic disruption) were also observed in the testicular cells of the beetles sampled from the polluted site compared to those sampled from the unpolluted site. Our results, therefore, advocate a need for adequate measures to reduce increasing environmental pollution in the urban-industrial areas.Lamia M El-SamadSaeed El-AshramDalia A KheirallahKarolin K Abdul-AzizNoura A TotoEl Hassan M MokhamerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0253238 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lamia M El-Samad Saeed El-Ashram Dalia A Kheirallah Karolin K Abdul-Aziz Noura A Toto El Hassan M Mokhamer Relative gene expression, micronuclei formation, and ultrastructure alterations induced by heavy metal contamination in Pimelia latreillei (Coleoptera: Tenebrionidae) in an urban-industrial area of Alexandria, Egypt.
description	The present research aims to evaluate the impact of industrial processes and anthropogenic activities on the beetle Pimelia latreillei inhabiting the polluted site at Zawya Abd El- Qader, Alexandria, Egypt. Beetles were collected from the vicinity of five factories. The genotoxic effects of environmental exposures to industrial heavy metals were monitored using a broad range of assays, including energy-dispersive X ray microanalysis and X-ray diffraction (SEM and EDX)), qRT-PCR gene expression assay, micronuclei formation, and transmission electron microscope (TEM). Energy dispersive X-ray microanalysis for the soil and testicular tissues of beetles collected from the polluted site revealed a higher percentage of heavy metals than the beetles collected from the reference site (Sidi Kirier, Alexandria, Egypt). To analyze/monitor genotoxicity in P. latreillei sampled from the polluted site, the transcription levels of levels of heat shock proteins (Hsps) and accessory gland seminal fluid protein (AcPC01) in testicular tissues were recorded. The incidence of micronuclei (MN) formation in the testicular cells was also observed. Quantitative RT-PCR (RT-qPCR) analysis was carried out to detect the changes in the gene expression of the aforementioned proteins. Genes encoding heat shock proteins (Hsp60, Hsp70, and Hsp90) were significantly overexpressed (> 2-fold) in specimens sampled from the polluted site; however, AcPC01 gene expression was under-expressed (<1.5-folds). The incidence of MN was significantly increased in specimens sampled from the polluted site. Ultrastructure anomalies (nuclear and cytoplasmic disruption) were also observed in the testicular cells of the beetles sampled from the polluted site compared to those sampled from the unpolluted site. Our results, therefore, advocate a need for adequate measures to reduce increasing environmental pollution in the urban-industrial areas.
format	article
author	Lamia M El-Samad Saeed El-Ashram Dalia A Kheirallah Karolin K Abdul-Aziz Noura A Toto El Hassan M Mokhamer
author_facet	Lamia M El-Samad Saeed El-Ashram Dalia A Kheirallah Karolin K Abdul-Aziz Noura A Toto El Hassan M Mokhamer
author_sort	Lamia M El-Samad
title	Relative gene expression, micronuclei formation, and ultrastructure alterations induced by heavy metal contamination in Pimelia latreillei (Coleoptera: Tenebrionidae) in an urban-industrial area of Alexandria, Egypt.
title_short	Relative gene expression, micronuclei formation, and ultrastructure alterations induced by heavy metal contamination in Pimelia latreillei (Coleoptera: Tenebrionidae) in an urban-industrial area of Alexandria, Egypt.
title_full	Relative gene expression, micronuclei formation, and ultrastructure alterations induced by heavy metal contamination in Pimelia latreillei (Coleoptera: Tenebrionidae) in an urban-industrial area of Alexandria, Egypt.
title_fullStr	Relative gene expression, micronuclei formation, and ultrastructure alterations induced by heavy metal contamination in Pimelia latreillei (Coleoptera: Tenebrionidae) in an urban-industrial area of Alexandria, Egypt.
title_full_unstemmed	Relative gene expression, micronuclei formation, and ultrastructure alterations induced by heavy metal contamination in Pimelia latreillei (Coleoptera: Tenebrionidae) in an urban-industrial area of Alexandria, Egypt.
title_sort	relative gene expression, micronuclei formation, and ultrastructure alterations induced by heavy metal contamination in pimelia latreillei (coleoptera: tenebrionidae) in an urban-industrial area of alexandria, egypt.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a459ab70decf4e63bd87edd3e112a21d
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Relative gene expression, micronuclei formation, and ultrastructure alterations induced by heavy metal contamination in Pimelia latreillei (Coleoptera: Tenebrionidae) in an urban-industrial area of Alexandria, Egypt.

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