Polyfunctional T cell responses in children in early stages of chronic Trypanosoma cruzi infection contrast with monofunctional responses of long-term infected adults.

<h4>Background</h4>Adults with chronic Trypanosoma cruzi exhibit a poorly functional T cell compartment, characterized by monofunctional (IFN-γ-only secreting) parasite-specific T cells and increased levels of terminally differentiated T cells. It is possible that persistent infection an...

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Autores principales:	María C Albareda, Ana M De Rissio, Gonzalo Tomas, Alicia Serjan, María G Alvarez, Rodolfo Viotti, Laura E Fichera, Mónica I Esteva, Daniel Potente, Alejandro Armenti, Rick L Tarleton, Susana A Laucella
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/a45df8bb769e448e918b841e2efa56f2
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spelling	oai:doaj.org-article:a45df8bb769e448e918b841e2efa56f22021-11-18T09:16:35ZPolyfunctional T cell responses in children in early stages of chronic Trypanosoma cruzi infection contrast with monofunctional responses of long-term infected adults.1935-27271935-273510.1371/journal.pntd.0002575https://doaj.org/article/a45df8bb769e448e918b841e2efa56f22013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349591/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>Adults with chronic Trypanosoma cruzi exhibit a poorly functional T cell compartment, characterized by monofunctional (IFN-γ-only secreting) parasite-specific T cells and increased levels of terminally differentiated T cells. It is possible that persistent infection and/or sustained exposure to parasites antigens may lead to a progressive loss of function of the immune T cells.<h4>Methodology/principal findings</h4>To test this hypothesis, the quality and magnitude of T. cruzi-specific T cell responses were evaluated in T. cruzi-infected children and compared with long-term T. cruzi-infected adults with no evidence of heart failure. The phenotype of CD4(+) T cells was also assessed in T. cruzi-infected children and uninfected controls. Simultaneous secretion of IFN-γ and IL-2 measured by ELISPOT assays in response to T. cruzi antigens was prevalent among T. cruzi-infected children. Flow cytometric analysis of co-expression profiles of CD4(+) T cells with the ability to produce IFN-γ, TNF-α, or to express the co-stimulatory molecule CD154 in response to T. cruzi showed polyfunctional T cell responses in most T. cruzi-infected children. Monofunctional T cell responses and an absence of CD4(+)TNF-α(+)-secreting T cells were observed in T. cruzi-infected adults. A relatively high degree of activation and differentiation of CD4(+) T cells was evident in T. cruzi-infected children.<h4>Conclusions/significance</h4>Our observations are compatible with our initial hypothesis that persistent T. cruzi infection promotes eventual exhaustion of immune system, which might contribute to disease progression in long-term infected subjects.María C AlbaredaAna M De RissioGonzalo TomasAlicia SerjanMaría G AlvarezRodolfo ViottiLaura E FicheraMónica I EstevaDaniel PotenteAlejandro ArmentiRick L TarletonSusana A LaucellaPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 7, Iss 12, p e2575 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 María C Albareda Ana M De Rissio Gonzalo Tomas Alicia Serjan María G Alvarez Rodolfo Viotti Laura E Fichera Mónica I Esteva Daniel Potente Alejandro Armenti Rick L Tarleton Susana A Laucella Polyfunctional T cell responses in children in early stages of chronic Trypanosoma cruzi infection contrast with monofunctional responses of long-term infected adults.
description	<h4>Background</h4>Adults with chronic Trypanosoma cruzi exhibit a poorly functional T cell compartment, characterized by monofunctional (IFN-γ-only secreting) parasite-specific T cells and increased levels of terminally differentiated T cells. It is possible that persistent infection and/or sustained exposure to parasites antigens may lead to a progressive loss of function of the immune T cells.<h4>Methodology/principal findings</h4>To test this hypothesis, the quality and magnitude of T. cruzi-specific T cell responses were evaluated in T. cruzi-infected children and compared with long-term T. cruzi-infected adults with no evidence of heart failure. The phenotype of CD4(+) T cells was also assessed in T. cruzi-infected children and uninfected controls. Simultaneous secretion of IFN-γ and IL-2 measured by ELISPOT assays in response to T. cruzi antigens was prevalent among T. cruzi-infected children. Flow cytometric analysis of co-expression profiles of CD4(+) T cells with the ability to produce IFN-γ, TNF-α, or to express the co-stimulatory molecule CD154 in response to T. cruzi showed polyfunctional T cell responses in most T. cruzi-infected children. Monofunctional T cell responses and an absence of CD4(+)TNF-α(+)-secreting T cells were observed in T. cruzi-infected adults. A relatively high degree of activation and differentiation of CD4(+) T cells was evident in T. cruzi-infected children.<h4>Conclusions/significance</h4>Our observations are compatible with our initial hypothesis that persistent T. cruzi infection promotes eventual exhaustion of immune system, which might contribute to disease progression in long-term infected subjects.
format	article
author	María C Albareda Ana M De Rissio Gonzalo Tomas Alicia Serjan María G Alvarez Rodolfo Viotti Laura E Fichera Mónica I Esteva Daniel Potente Alejandro Armenti Rick L Tarleton Susana A Laucella
author_facet	María C Albareda Ana M De Rissio Gonzalo Tomas Alicia Serjan María G Alvarez Rodolfo Viotti Laura E Fichera Mónica I Esteva Daniel Potente Alejandro Armenti Rick L Tarleton Susana A Laucella
author_sort	María C Albareda
title	Polyfunctional T cell responses in children in early stages of chronic Trypanosoma cruzi infection contrast with monofunctional responses of long-term infected adults.
title_short	Polyfunctional T cell responses in children in early stages of chronic Trypanosoma cruzi infection contrast with monofunctional responses of long-term infected adults.
title_full	Polyfunctional T cell responses in children in early stages of chronic Trypanosoma cruzi infection contrast with monofunctional responses of long-term infected adults.
title_fullStr	Polyfunctional T cell responses in children in early stages of chronic Trypanosoma cruzi infection contrast with monofunctional responses of long-term infected adults.
title_full_unstemmed	Polyfunctional T cell responses in children in early stages of chronic Trypanosoma cruzi infection contrast with monofunctional responses of long-term infected adults.
title_sort	polyfunctional t cell responses in children in early stages of chronic trypanosoma cruzi infection contrast with monofunctional responses of long-term infected adults.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a45df8bb769e448e918b841e2efa56f2
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Polyfunctional T cell responses in children in early stages of chronic Trypanosoma cruzi infection contrast with monofunctional responses of long-term infected adults.

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