Baculovirus Vectors Induce the Production of Interferons in Swine: Their Potential in the Development of Antiviral Strategies

The huge variety of viruses affecting swine represents a global threat. Since vaccines against highly contagious viruses last several days to induce protective immune responses, antiviral strategies for rapid control of outbreak situations are needed. The baculovirus <i>Autographa californica&...

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Autores principales:	Guido Nicolás Molina, Sabrina Amalfi, Ignacio Otero, Oscar Taboga, María Paula Molinari
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	baculovirus interferon swine antiviral Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/a4652dba0363478490281f2f1ea502d2
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spelling	oai:doaj.org-article:a4652dba0363478490281f2f1ea502d22021-11-25T19:12:07ZBaculovirus Vectors Induce the Production of Interferons in Swine: Their Potential in the Development of Antiviral Strategies10.3390/vetsci81102782306-7381https://doaj.org/article/a4652dba0363478490281f2f1ea502d22021-11-01T00:00:00Zhttps://www.mdpi.com/2306-7381/8/11/278https://doaj.org/toc/2306-7381The huge variety of viruses affecting swine represents a global threat. Since vaccines against highly contagious viruses last several days to induce protective immune responses, antiviral strategies for rapid control of outbreak situations are needed. The baculovirus <i>Autographa californica</i> multiple nucleopolyhedrovirus (AcMNPV), an insect virus, has been demonstrated to be an effective vaccine vector for mammals. Besides the ability to display or transduce heterologous antigens, it also induces strong innate immune responses and provides IFN-mediated protection against lethal challenges with viruses like foot-and-mouth disease virus (FMDV) in mice. Thus, the aim of this study was to evaluate the ability of AcMNPV to induce IFN production and elicit antiviral activity in porcine peripheral blood mononuclear cells (PBMCs). Our results demonstrated that AcMNPV induced an IFN-α-mediated antiviral activity in PBMCs in vitro. Moreover, the inoculation of AcMNPV in piglets led to the production of type I and II IFNs in sera from inoculated animals and antiviral activities against vesicular stomatitis virus (VSV) and FMDV measured by in vitro assays. Finally, it was demonstrated that the pseudotyping of AcMNPV with VSV-G protein, but not the enrichment of the AcMNPV genome with specific immunostimulatory CpG motifs for the porcine TLR9, improved the ability to induce IFN-α production in PBMCs in vitro. Together, these results suggest that AcMNPV is a promising tool for the induction of IFNs in antiviral strategies, with the potential to be biotechnologically improved.Guido Nicolás MolinaSabrina AmalfiIgnacio OteroOscar TabogaMaría Paula MolinariMDPI AGarticlebaculovirusinterferonswineantiviralVeterinary medicineSF600-1100ENVeterinary Sciences, Vol 8, Iss 278, p 278 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	baculovirus interferon swine antiviral Veterinary medicine SF600-1100
spellingShingle	baculovirus interferon swine antiviral Veterinary medicine SF600-1100 Guido Nicolás Molina Sabrina Amalfi Ignacio Otero Oscar Taboga María Paula Molinari Baculovirus Vectors Induce the Production of Interferons in Swine: Their Potential in the Development of Antiviral Strategies
description	The huge variety of viruses affecting swine represents a global threat. Since vaccines against highly contagious viruses last several days to induce protective immune responses, antiviral strategies for rapid control of outbreak situations are needed. The baculovirus <i>Autographa californica</i> multiple nucleopolyhedrovirus (AcMNPV), an insect virus, has been demonstrated to be an effective vaccine vector for mammals. Besides the ability to display or transduce heterologous antigens, it also induces strong innate immune responses and provides IFN-mediated protection against lethal challenges with viruses like foot-and-mouth disease virus (FMDV) in mice. Thus, the aim of this study was to evaluate the ability of AcMNPV to induce IFN production and elicit antiviral activity in porcine peripheral blood mononuclear cells (PBMCs). Our results demonstrated that AcMNPV induced an IFN-α-mediated antiviral activity in PBMCs in vitro. Moreover, the inoculation of AcMNPV in piglets led to the production of type I and II IFNs in sera from inoculated animals and antiviral activities against vesicular stomatitis virus (VSV) and FMDV measured by in vitro assays. Finally, it was demonstrated that the pseudotyping of AcMNPV with VSV-G protein, but not the enrichment of the AcMNPV genome with specific immunostimulatory CpG motifs for the porcine TLR9, improved the ability to induce IFN-α production in PBMCs in vitro. Together, these results suggest that AcMNPV is a promising tool for the induction of IFNs in antiviral strategies, with the potential to be biotechnologically improved.
format	article
author	Guido Nicolás Molina Sabrina Amalfi Ignacio Otero Oscar Taboga María Paula Molinari
author_facet	Guido Nicolás Molina Sabrina Amalfi Ignacio Otero Oscar Taboga María Paula Molinari
author_sort	Guido Nicolás Molina
title	Baculovirus Vectors Induce the Production of Interferons in Swine: Their Potential in the Development of Antiviral Strategies
title_short	Baculovirus Vectors Induce the Production of Interferons in Swine: Their Potential in the Development of Antiviral Strategies
title_full	Baculovirus Vectors Induce the Production of Interferons in Swine: Their Potential in the Development of Antiviral Strategies
title_fullStr	Baculovirus Vectors Induce the Production of Interferons in Swine: Their Potential in the Development of Antiviral Strategies
title_full_unstemmed	Baculovirus Vectors Induce the Production of Interferons in Swine: Their Potential in the Development of Antiviral Strategies
title_sort	baculovirus vectors induce the production of interferons in swine: their potential in the development of antiviral strategies
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a4652dba0363478490281f2f1ea502d2
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Baculovirus Vectors Induce the Production of Interferons in Swine: Their Potential in the Development of Antiviral Strategies

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