Neutrophil degranulation interconnects over-represented biological processes in atrial fibrillation
Abstract Despite our expanding knowledge about the mechanism underlying atrial fibrillation (AF), the interplay between the biological events underlying AF remains incompletely understood. This study aimed to identify the functionally enriched gene-sets in AF and capture their interconnection via pi...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/a465c257cf0a4202abba46855f955d7d |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:a465c257cf0a4202abba46855f955d7d |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:a465c257cf0a4202abba46855f955d7d2021-12-02T10:44:21ZNeutrophil degranulation interconnects over-represented biological processes in atrial fibrillation10.1038/s41598-021-82533-52045-2322https://doaj.org/article/a465c257cf0a4202abba46855f955d7d2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82533-5https://doaj.org/toc/2045-2322Abstract Despite our expanding knowledge about the mechanism underlying atrial fibrillation (AF), the interplay between the biological events underlying AF remains incompletely understood. This study aimed to identify the functionally enriched gene-sets in AF and capture their interconnection via pivotal factors, that may drive or be driven by AF. Global abundance of the proteins in the left atrium of AF patients compared to control patients (n = 3/group), and the functionally enriched biological processes in AF were determined by mass-spectrometry and gene set enrichment analysis, respectively. The data were validated in an independent cohort (n = 19–20/group). In AF, the gene-sets of innate immune system, metabolic process, cellular component disassembly and ion homeostasis were up-regulated, while the gene-set of ciliogenesis was down-regulated. The innate immune system was over-represented by neutrophil degranulation, the components of which were extensively shared by other gene-sets altered in AF. In the independent cohort, an activated form of neutrophils was more present in the left atrium of AF patients with the increased gene expression of neutrophil granules. MYH10, required for ciliogenesis, was decreased in the atrial fibroblasts of AF patients. We report the increased neutrophil degranulation appears to play a pivotal role, and affects multiple biological processes altered in AF.Makiri KawasakiEva R. MeulendijksNicoline W. E. van den BergFransisca A. NariswariJolien NeefsRobin WesselinkSarah W. E. BaalmanAldo JongejanTim SchelfhorstSander R. PiersmaThang V. PhamWim J. P. van BovenAntoine H. G. DriessenConnie R. JimenezJoris R. de GrootNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Makiri Kawasaki Eva R. Meulendijks Nicoline W. E. van den Berg Fransisca A. Nariswari Jolien Neefs Robin Wesselink Sarah W. E. Baalman Aldo Jongejan Tim Schelfhorst Sander R. Piersma Thang V. Pham Wim J. P. van Boven Antoine H. G. Driessen Connie R. Jimenez Joris R. de Groot Neutrophil degranulation interconnects over-represented biological processes in atrial fibrillation |
| description |
Abstract Despite our expanding knowledge about the mechanism underlying atrial fibrillation (AF), the interplay between the biological events underlying AF remains incompletely understood. This study aimed to identify the functionally enriched gene-sets in AF and capture their interconnection via pivotal factors, that may drive or be driven by AF. Global abundance of the proteins in the left atrium of AF patients compared to control patients (n = 3/group), and the functionally enriched biological processes in AF were determined by mass-spectrometry and gene set enrichment analysis, respectively. The data were validated in an independent cohort (n = 19–20/group). In AF, the gene-sets of innate immune system, metabolic process, cellular component disassembly and ion homeostasis were up-regulated, while the gene-set of ciliogenesis was down-regulated. The innate immune system was over-represented by neutrophil degranulation, the components of which were extensively shared by other gene-sets altered in AF. In the independent cohort, an activated form of neutrophils was more present in the left atrium of AF patients with the increased gene expression of neutrophil granules. MYH10, required for ciliogenesis, was decreased in the atrial fibroblasts of AF patients. We report the increased neutrophil degranulation appears to play a pivotal role, and affects multiple biological processes altered in AF. |
| format |
article |
| author |
Makiri Kawasaki Eva R. Meulendijks Nicoline W. E. van den Berg Fransisca A. Nariswari Jolien Neefs Robin Wesselink Sarah W. E. Baalman Aldo Jongejan Tim Schelfhorst Sander R. Piersma Thang V. Pham Wim J. P. van Boven Antoine H. G. Driessen Connie R. Jimenez Joris R. de Groot |
| author_facet |
Makiri Kawasaki Eva R. Meulendijks Nicoline W. E. van den Berg Fransisca A. Nariswari Jolien Neefs Robin Wesselink Sarah W. E. Baalman Aldo Jongejan Tim Schelfhorst Sander R. Piersma Thang V. Pham Wim J. P. van Boven Antoine H. G. Driessen Connie R. Jimenez Joris R. de Groot |
| author_sort |
Makiri Kawasaki |
| title |
Neutrophil degranulation interconnects over-represented biological processes in atrial fibrillation |
| title_short |
Neutrophil degranulation interconnects over-represented biological processes in atrial fibrillation |
| title_full |
Neutrophil degranulation interconnects over-represented biological processes in atrial fibrillation |
| title_fullStr |
Neutrophil degranulation interconnects over-represented biological processes in atrial fibrillation |
| title_full_unstemmed |
Neutrophil degranulation interconnects over-represented biological processes in atrial fibrillation |
| title_sort |
neutrophil degranulation interconnects over-represented biological processes in atrial fibrillation |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/a465c257cf0a4202abba46855f955d7d |
| work_keys_str_mv |
AT makirikawasaki neutrophildegranulationinterconnectsoverrepresentedbiologicalprocessesinatrialfibrillation AT evarmeulendijks neutrophildegranulationinterconnectsoverrepresentedbiologicalprocessesinatrialfibrillation AT nicolinewevandenberg neutrophildegranulationinterconnectsoverrepresentedbiologicalprocessesinatrialfibrillation AT fransiscaanariswari neutrophildegranulationinterconnectsoverrepresentedbiologicalprocessesinatrialfibrillation AT jolienneefs neutrophildegranulationinterconnectsoverrepresentedbiologicalprocessesinatrialfibrillation AT robinwesselink neutrophildegranulationinterconnectsoverrepresentedbiologicalprocessesinatrialfibrillation AT sarahwebaalman neutrophildegranulationinterconnectsoverrepresentedbiologicalprocessesinatrialfibrillation AT aldojongejan neutrophildegranulationinterconnectsoverrepresentedbiologicalprocessesinatrialfibrillation AT timschelfhorst neutrophildegranulationinterconnectsoverrepresentedbiologicalprocessesinatrialfibrillation AT sanderrpiersma neutrophildegranulationinterconnectsoverrepresentedbiologicalprocessesinatrialfibrillation AT thangvpham neutrophildegranulationinterconnectsoverrepresentedbiologicalprocessesinatrialfibrillation AT wimjpvanboven neutrophildegranulationinterconnectsoverrepresentedbiologicalprocessesinatrialfibrillation AT antoinehgdriessen neutrophildegranulationinterconnectsoverrepresentedbiologicalprocessesinatrialfibrillation AT connierjimenez neutrophildegranulationinterconnectsoverrepresentedbiologicalprocessesinatrialfibrillation AT jorisrdegroot neutrophildegranulationinterconnectsoverrepresentedbiologicalprocessesinatrialfibrillation |
| _version_ |
1718396781999423488 |