Growth of Acinetobacter baumannii in pellicle enhanced the expression of potential virulence factors.

<h4>Background</h4>Interestingly, Acinetobacter baumannii presents an enhanced capacity to form biofilms (also named pellicles) at the air-liquid interface as compared to the other Acinetobacter species. This characteristic questions the contribution of this phenotype to an increased ris...

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Autores principales:	Sara Marti, Yassine Nait Chabane, Stéphane Alexandre, Laurent Coquet, Jordi Vila, Thierry Jouenne, Emmanuelle Dé
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a469b5c790ec414eb38102912753cc13
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spelling	oai:doaj.org-article:a469b5c790ec414eb38102912753cc132021-11-18T07:35:38ZGrowth of Acinetobacter baumannii in pellicle enhanced the expression of potential virulence factors.1932-620310.1371/journal.pone.0026030https://doaj.org/article/a469b5c790ec414eb38102912753cc132011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22046254/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Interestingly, Acinetobacter baumannii presents an enhanced capacity to form biofilms (also named pellicles) at the air-liquid interface as compared to the other Acinetobacter species. This characteristic questions the contribution of this phenotype to an increased risk of clinical infections by this pathogen.<h4>Methodology/principal findings</h4>By a proteomic approach using 2-D gel electrophoresis-LC-MS/MS mass spectrometry, we compared the membrane protein patterns of A. baumannii 77, a pellicle-forming clinical isolate, grown in planktonic and in sessile modes. We identified 52 proteins with a differential expression, including 32 up-regulated and 20 down-regulated in the pellicle state. Several proteins, differentially expressed during pellicle development, were of particular interest. We determined the over-expression of four siderophore iron uptake systems including the acinetobactin and enterobactin receptors and confirmed that the development of this type of biofilm is promoted by ferric ions. Two over-expressed proteins, CarO and an OprD-homologue, putative carbapenem-resistance associated porins, would be involved in the transport of specific compounds, like ornithine, a biosynthesis precursor of a siderophore from the hydroxamate family. We evidenced the overexpression of a lipase and a transporter of LCFA that may be involved in the recycling of lipids inside the pellicle matrix. Finally, we demonstrated both by proteomic and by AFM studies that this particular type of biofilm required multiple pili systems to maintain this cohesive structure at the air-liquid interface; two of these systems have never been described in A. baumannii.<h4>Conclusions/significance</h4>Our study demonstrated that several proteins, overexpressed at a late state of pellicle development, could be potentially involved in virulence processes. Therefore, regarding the number of potential virulence factors that are over-expressed in this growth mode, the pellicle-forming clinical isolates should be kept under survey.Sara MartiYassine Nait ChabaneStéphane AlexandreLaurent CoquetJordi VilaThierry JouenneEmmanuelle DéPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e26030 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sara Marti Yassine Nait Chabane Stéphane Alexandre Laurent Coquet Jordi Vila Thierry Jouenne Emmanuelle Dé Growth of Acinetobacter baumannii in pellicle enhanced the expression of potential virulence factors.
description	<h4>Background</h4>Interestingly, Acinetobacter baumannii presents an enhanced capacity to form biofilms (also named pellicles) at the air-liquid interface as compared to the other Acinetobacter species. This characteristic questions the contribution of this phenotype to an increased risk of clinical infections by this pathogen.<h4>Methodology/principal findings</h4>By a proteomic approach using 2-D gel electrophoresis-LC-MS/MS mass spectrometry, we compared the membrane protein patterns of A. baumannii 77, a pellicle-forming clinical isolate, grown in planktonic and in sessile modes. We identified 52 proteins with a differential expression, including 32 up-regulated and 20 down-regulated in the pellicle state. Several proteins, differentially expressed during pellicle development, were of particular interest. We determined the over-expression of four siderophore iron uptake systems including the acinetobactin and enterobactin receptors and confirmed that the development of this type of biofilm is promoted by ferric ions. Two over-expressed proteins, CarO and an OprD-homologue, putative carbapenem-resistance associated porins, would be involved in the transport of specific compounds, like ornithine, a biosynthesis precursor of a siderophore from the hydroxamate family. We evidenced the overexpression of a lipase and a transporter of LCFA that may be involved in the recycling of lipids inside the pellicle matrix. Finally, we demonstrated both by proteomic and by AFM studies that this particular type of biofilm required multiple pili systems to maintain this cohesive structure at the air-liquid interface; two of these systems have never been described in A. baumannii.<h4>Conclusions/significance</h4>Our study demonstrated that several proteins, overexpressed at a late state of pellicle development, could be potentially involved in virulence processes. Therefore, regarding the number of potential virulence factors that are over-expressed in this growth mode, the pellicle-forming clinical isolates should be kept under survey.
format	article
author	Sara Marti Yassine Nait Chabane Stéphane Alexandre Laurent Coquet Jordi Vila Thierry Jouenne Emmanuelle Dé
author_facet	Sara Marti Yassine Nait Chabane Stéphane Alexandre Laurent Coquet Jordi Vila Thierry Jouenne Emmanuelle Dé
author_sort	Sara Marti
title	Growth of Acinetobacter baumannii in pellicle enhanced the expression of potential virulence factors.
title_short	Growth of Acinetobacter baumannii in pellicle enhanced the expression of potential virulence factors.
title_full	Growth of Acinetobacter baumannii in pellicle enhanced the expression of potential virulence factors.
title_fullStr	Growth of Acinetobacter baumannii in pellicle enhanced the expression of potential virulence factors.
title_full_unstemmed	Growth of Acinetobacter baumannii in pellicle enhanced the expression of potential virulence factors.
title_sort	growth of acinetobacter baumannii in pellicle enhanced the expression of potential virulence factors.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/a469b5c790ec414eb38102912753cc13
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Growth of Acinetobacter baumannii in pellicle enhanced the expression of potential virulence factors.

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