Green quantitative spectrofluorometric analysis of rupatadine and montelukast at nanogram scale using direct and synchronous techniques
Two green-sensitive spectrofluorometric methods were investigated for assay of rupatadine (RUP) [method I] and its binary mixture with montelukast (MKT) [method II]. Method I depends on measuring native fluorescence of RUP in the presence of 0.10 M H2SO4 and 0.10%w/v sodium dodecyl sulfate at 455 nm...
Guardado en:
Autores principales: | , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
The Royal Society
2021
|
Materias: | |
Acceso en línea: | https://doaj.org/article/a46cd60db20a4d92a4974821adc9db7b |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:a46cd60db20a4d92a4974821adc9db7b |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:a46cd60db20a4d92a4974821adc9db7b2021-11-10T08:06:33ZGreen quantitative spectrofluorometric analysis of rupatadine and montelukast at nanogram scale using direct and synchronous techniques10.1098/rsos.2111962054-5703https://doaj.org/article/a46cd60db20a4d92a4974821adc9db7b2021-11-01T00:00:00Zhttps://royalsocietypublishing.org/doi/10.1098/rsos.211196https://doaj.org/toc/2054-5703Two green-sensitive spectrofluorometric methods were investigated for assay of rupatadine (RUP) [method I] and its binary mixture with montelukast (MKT) [method II]. Method I depends on measuring native fluorescence of RUP in the presence of 0.10 M H2SO4 and 0.10%w/v sodium dodecyl sulfate at 455 nm after excitation at 277 nm. The range of the first method was 0.20–2.00 µg ml−1 with detection and quantitation limits of 59.00 and 179.00 ng ml−1, respectively. Method II depends on the first derivative synchronous spectrofluorometry. The derivative intensities were measured for the two drugs in an aqueous solution containing Mcllvaine's buffer pH 2.60 at fixed Δλ of 140 nm. Each drug was estimated at zero-contribution of the other. The intensity was measured at 261 and 371 nm for RUP and MKT, respectively. The method was linear over 0.10–4.00 and 0.20–1.60 µg ml−1 with limits of detection 31.00 and 66.00 ng ml−1 and limits of quantitation 94.00 and 200.00 ng ml−1 for RUP and MKT, respectively. The method was extended to determine this mixture in laboratory-prepared mixtures and combined tablets. Method validation was performed according to ICH guidelines. Statistical interpretation of data revealed good agreement with the comparison method. Method greenness was confirmed by applying three different assessment tools.Rana GhonimMohamed I. El-AwadyManar M. TolbaFawzia IbrahimThe Royal Societyarticlerupatadinemontelukastnative fluorescencederivative synchronous fluorescence spectroscopypharmaceutical dosage formsScienceQENRoyal Society Open Science, Vol 8, Iss 11 (2021) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
rupatadine montelukast native fluorescence derivative synchronous fluorescence spectroscopy pharmaceutical dosage forms Science Q |
spellingShingle |
rupatadine montelukast native fluorescence derivative synchronous fluorescence spectroscopy pharmaceutical dosage forms Science Q Rana Ghonim Mohamed I. El-Awady Manar M. Tolba Fawzia Ibrahim Green quantitative spectrofluorometric analysis of rupatadine and montelukast at nanogram scale using direct and synchronous techniques |
description |
Two green-sensitive spectrofluorometric methods were investigated for assay of rupatadine (RUP) [method I] and its binary mixture with montelukast (MKT) [method II]. Method I depends on measuring native fluorescence of RUP in the presence of 0.10 M H2SO4 and 0.10%w/v sodium dodecyl sulfate at 455 nm after excitation at 277 nm. The range of the first method was 0.20–2.00 µg ml−1 with detection and quantitation limits of 59.00 and 179.00 ng ml−1, respectively. Method II depends on the first derivative synchronous spectrofluorometry. The derivative intensities were measured for the two drugs in an aqueous solution containing Mcllvaine's buffer pH 2.60 at fixed Δλ of 140 nm. Each drug was estimated at zero-contribution of the other. The intensity was measured at 261 and 371 nm for RUP and MKT, respectively. The method was linear over 0.10–4.00 and 0.20–1.60 µg ml−1 with limits of detection 31.00 and 66.00 ng ml−1 and limits of quantitation 94.00 and 200.00 ng ml−1 for RUP and MKT, respectively. The method was extended to determine this mixture in laboratory-prepared mixtures and combined tablets. Method validation was performed according to ICH guidelines. Statistical interpretation of data revealed good agreement with the comparison method. Method greenness was confirmed by applying three different assessment tools. |
format |
article |
author |
Rana Ghonim Mohamed I. El-Awady Manar M. Tolba Fawzia Ibrahim |
author_facet |
Rana Ghonim Mohamed I. El-Awady Manar M. Tolba Fawzia Ibrahim |
author_sort |
Rana Ghonim |
title |
Green quantitative spectrofluorometric analysis of rupatadine and montelukast at nanogram scale using direct and synchronous techniques |
title_short |
Green quantitative spectrofluorometric analysis of rupatadine and montelukast at nanogram scale using direct and synchronous techniques |
title_full |
Green quantitative spectrofluorometric analysis of rupatadine and montelukast at nanogram scale using direct and synchronous techniques |
title_fullStr |
Green quantitative spectrofluorometric analysis of rupatadine and montelukast at nanogram scale using direct and synchronous techniques |
title_full_unstemmed |
Green quantitative spectrofluorometric analysis of rupatadine and montelukast at nanogram scale using direct and synchronous techniques |
title_sort |
green quantitative spectrofluorometric analysis of rupatadine and montelukast at nanogram scale using direct and synchronous techniques |
publisher |
The Royal Society |
publishDate |
2021 |
url |
https://doaj.org/article/a46cd60db20a4d92a4974821adc9db7b |
work_keys_str_mv |
AT ranaghonim greenquantitativespectrofluorometricanalysisofrupatadineandmontelukastatnanogramscaleusingdirectandsynchronoustechniques AT mohamedielawady greenquantitativespectrofluorometricanalysisofrupatadineandmontelukastatnanogramscaleusingdirectandsynchronoustechniques AT manarmtolba greenquantitativespectrofluorometricanalysisofrupatadineandmontelukastatnanogramscaleusingdirectandsynchronoustechniques AT fawziaibrahim greenquantitativespectrofluorometricanalysisofrupatadineandmontelukastatnanogramscaleusingdirectandsynchronoustechniques |
_version_ |
1718440405243002880 |