Skeletal myotube formation enhanced by electrospun polyurethane carbon nanotube scaffolds

Sirinrath Sirivisoot, Benjamin S Harrison Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Boulevard, Winston-Salem, NC, USA Background: This study examined the effects of electrically conductive materials made from electrospun single- or multiw...

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Autores principales:	Sirivisoot S, Harrison BS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a47395e3ac884bc587729ee56b152a3e
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spelling	oai:doaj.org-article:a47395e3ac884bc587729ee56b152a3e2021-12-02T01:42:37ZSkeletal myotube formation enhanced by electrospun polyurethane carbon nanotube scaffolds1176-91141178-2013https://doaj.org/article/a47395e3ac884bc587729ee56b152a3e2011-10-01T00:00:00Zhttp://www.dovepress.com/skeletal-myotube-formation-enhanced-by-electrospun-polyurethane-carbon-a8514https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Sirinrath Sirivisoot, Benjamin S Harrison Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Boulevard, Winston-Salem, NC, USA Background: This study examined the effects of electrically conductive materials made from electrospun single- or multiwalled carbon nanotubes with polyurethane to promote myoblast differentiation into myotubes in the presence and absence of electrical stimulation. Methods and results: After electrical stimulation, the number of multinucleated myotubes on the electrospun polyurethane carbon nanotube scaffolds was significantly larger than that on nonconductive electrospun polyurethane scaffolds (5% and 10% w/v polyurethane). In the absence of electrical stimulation, myoblasts also differentiated on the electrospun polyurethane carbon nanotube scaffolds, as evidenced by expression of Myf-5 and myosin heavy chains. The myotube number and length were significantly greater on the electrospun carbon nanotubes with 10% w/v polyurethane than on those with 5% w/v polyurethane. The results suggest that, in the absence of electrical stimulation, skeletal myotube formation is dependent on the morphology of the electrospun scaffolds, while with electrical stimulation it is dependent on the electrical conductivity of the scaffolds. Conclusion: This study indicates that electrospun polyurethane carbon nanotubes can be used to modulate skeletal myotube formation with or without application of electrical stimulation. Keywords: myoblasts, electrical field, single-walled, multiwalled, carbon nanotubes, nanocomposites, musculoskeletal, tissue engineeringSirivisoot SHarrison BSDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2483-2497 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Sirivisoot S Harrison BS Skeletal myotube formation enhanced by electrospun polyurethane carbon nanotube scaffolds
description	Sirinrath Sirivisoot, Benjamin S Harrison Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Boulevard, Winston-Salem, NC, USA Background: This study examined the effects of electrically conductive materials made from electrospun single- or multiwalled carbon nanotubes with polyurethane to promote myoblast differentiation into myotubes in the presence and absence of electrical stimulation. Methods and results: After electrical stimulation, the number of multinucleated myotubes on the electrospun polyurethane carbon nanotube scaffolds was significantly larger than that on nonconductive electrospun polyurethane scaffolds (5% and 10% w/v polyurethane). In the absence of electrical stimulation, myoblasts also differentiated on the electrospun polyurethane carbon nanotube scaffolds, as evidenced by expression of Myf-5 and myosin heavy chains. The myotube number and length were significantly greater on the electrospun carbon nanotubes with 10% w/v polyurethane than on those with 5% w/v polyurethane. The results suggest that, in the absence of electrical stimulation, skeletal myotube formation is dependent on the morphology of the electrospun scaffolds, while with electrical stimulation it is dependent on the electrical conductivity of the scaffolds. Conclusion: This study indicates that electrospun polyurethane carbon nanotubes can be used to modulate skeletal myotube formation with or without application of electrical stimulation. Keywords: myoblasts, electrical field, single-walled, multiwalled, carbon nanotubes, nanocomposites, musculoskeletal, tissue engineering
format	article
author	Sirivisoot S Harrison BS
author_facet	Sirivisoot S Harrison BS
author_sort	Sirivisoot S
title	Skeletal myotube formation enhanced by electrospun polyurethane carbon nanotube scaffolds
title_short	Skeletal myotube formation enhanced by electrospun polyurethane carbon nanotube scaffolds
title_full	Skeletal myotube formation enhanced by electrospun polyurethane carbon nanotube scaffolds
title_fullStr	Skeletal myotube formation enhanced by electrospun polyurethane carbon nanotube scaffolds
title_full_unstemmed	Skeletal myotube formation enhanced by electrospun polyurethane carbon nanotube scaffolds
title_sort	skeletal myotube formation enhanced by electrospun polyurethane carbon nanotube scaffolds
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/a47395e3ac884bc587729ee56b152a3e
work_keys_str_mv	AT sirivisoots skeletalmyotubeformationenhancedbyelectrospunpolyurethanecarbonnanotubescaffolds AT harrisonbs skeletalmyotubeformationenhancedbyelectrospunpolyurethanecarbonnanotubescaffolds
_version_	1718402912696139776

Skeletal myotube formation enhanced by electrospun polyurethane carbon nanotube scaffolds

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