Expression of canine distemper virus receptor nectin-4 in the central nervous system of dogs

Abstract Canine distemper virus (CDV) exhibits lymphotropic, epitheliotropic, and neurotropic nature, and causes a severe systemic infection in susceptible animals. Initially, signaling lymphocyte activation molecule (SLAM) expressed on immune cells has been identified as a crucial cellular receptor...

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Autores principales:	Watanyoo Pratakpiriya, Angeline Ping Ping Teh, Araya Radtanakatikanon, Nopadon Pirarat, Nguyen Thi Lan, Makoto Takeda, Somporn Techangamsuwan, Ryoji Yamaguchi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a476b1dfb3354bfeb409e7b5b114beb8
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spelling	oai:doaj.org-article:a476b1dfb3354bfeb409e7b5b114beb82021-12-02T12:32:12ZExpression of canine distemper virus receptor nectin-4 in the central nervous system of dogs10.1038/s41598-017-00375-62045-2322https://doaj.org/article/a476b1dfb3354bfeb409e7b5b114beb82017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00375-6https://doaj.org/toc/2045-2322Abstract Canine distemper virus (CDV) exhibits lymphotropic, epitheliotropic, and neurotropic nature, and causes a severe systemic infection in susceptible animals. Initially, signaling lymphocyte activation molecule (SLAM) expressed on immune cells has been identified as a crucial cellular receptor for CDV. Currently, nectin-4 expressed in epithelia has been shown to be another receptor for CDV. Our previous study demonstrated that neurons express nectin-4 and are infected with CDV. In this study, we investigated the distribution pattern of nectin-4 in various cell types in the canine central nervous system and showed its relation to CDV infection to further clarify the pathology of disease. Histopathological, immunohistochemical and immunofluorescent analyses were done using formalin-fixed paraffin-embedded tissues of CDV-infected dogs. Dual staining of nectin-4 and CDV antigen or nectin-4 and brain cell markers was performed. Nectin-4 was detected in ependymal cells, epithelia of choroid plexus, meningeal cells, neurons, granular cells, and Purkinje’s cells. CDV antigens were detected in these nectin-4-positive cells, further suggesting contribution of nectin-4 for the CDV neurovirulence. On the other hand, astrocytes did not express nectin-4, although they were frequently infected with CDV. Since astrocytes are negative for SLAM expression, they must express an unidentified CDV receptor, which also contributes to CDV neurovirulence.Watanyoo PratakpiriyaAngeline Ping Ping TehAraya RadtanakatikanonNopadon PiraratNguyen Thi LanMakoto TakedaSomporn TechangamsuwanRyoji YamaguchiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Watanyoo Pratakpiriya Angeline Ping Ping Teh Araya Radtanakatikanon Nopadon Pirarat Nguyen Thi Lan Makoto Takeda Somporn Techangamsuwan Ryoji Yamaguchi Expression of canine distemper virus receptor nectin-4 in the central nervous system of dogs
description	Abstract Canine distemper virus (CDV) exhibits lymphotropic, epitheliotropic, and neurotropic nature, and causes a severe systemic infection in susceptible animals. Initially, signaling lymphocyte activation molecule (SLAM) expressed on immune cells has been identified as a crucial cellular receptor for CDV. Currently, nectin-4 expressed in epithelia has been shown to be another receptor for CDV. Our previous study demonstrated that neurons express nectin-4 and are infected with CDV. In this study, we investigated the distribution pattern of nectin-4 in various cell types in the canine central nervous system and showed its relation to CDV infection to further clarify the pathology of disease. Histopathological, immunohistochemical and immunofluorescent analyses were done using formalin-fixed paraffin-embedded tissues of CDV-infected dogs. Dual staining of nectin-4 and CDV antigen or nectin-4 and brain cell markers was performed. Nectin-4 was detected in ependymal cells, epithelia of choroid plexus, meningeal cells, neurons, granular cells, and Purkinje’s cells. CDV antigens were detected in these nectin-4-positive cells, further suggesting contribution of nectin-4 for the CDV neurovirulence. On the other hand, astrocytes did not express nectin-4, although they were frequently infected with CDV. Since astrocytes are negative for SLAM expression, they must express an unidentified CDV receptor, which also contributes to CDV neurovirulence.
format	article
author	Watanyoo Pratakpiriya Angeline Ping Ping Teh Araya Radtanakatikanon Nopadon Pirarat Nguyen Thi Lan Makoto Takeda Somporn Techangamsuwan Ryoji Yamaguchi
author_facet	Watanyoo Pratakpiriya Angeline Ping Ping Teh Araya Radtanakatikanon Nopadon Pirarat Nguyen Thi Lan Makoto Takeda Somporn Techangamsuwan Ryoji Yamaguchi
author_sort	Watanyoo Pratakpiriya
title	Expression of canine distemper virus receptor nectin-4 in the central nervous system of dogs
title_short	Expression of canine distemper virus receptor nectin-4 in the central nervous system of dogs
title_full	Expression of canine distemper virus receptor nectin-4 in the central nervous system of dogs
title_fullStr	Expression of canine distemper virus receptor nectin-4 in the central nervous system of dogs
title_full_unstemmed	Expression of canine distemper virus receptor nectin-4 in the central nervous system of dogs
title_sort	expression of canine distemper virus receptor nectin-4 in the central nervous system of dogs
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a476b1dfb3354bfeb409e7b5b114beb8
work_keys_str_mv	AT watanyoopratakpiriya expressionofcaninedistempervirusreceptornectin4inthecentralnervoussystemofdogs AT angelinepingpingteh expressionofcaninedistempervirusreceptornectin4inthecentralnervoussystemofdogs AT arayaradtanakatikanon expressionofcaninedistempervirusreceptornectin4inthecentralnervoussystemofdogs AT nopadonpirarat expressionofcaninedistempervirusreceptornectin4inthecentralnervoussystemofdogs AT nguyenthilan expressionofcaninedistempervirusreceptornectin4inthecentralnervoussystemofdogs AT makototakeda expressionofcaninedistempervirusreceptornectin4inthecentralnervoussystemofdogs AT somporntechangamsuwan expressionofcaninedistempervirusreceptornectin4inthecentralnervoussystemofdogs AT ryojiyamaguchi expressionofcaninedistempervirusreceptornectin4inthecentralnervoussystemofdogs
_version_	1718394176147554304

Expression of canine distemper virus receptor nectin-4 in the central nervous system of dogs

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