Membrane bound monomer of Staphylococcal alpha-hemolysin induces caspase activation and apoptotic cell death despite initiation of membrane repair pathway.

<h4>Background</h4>Wild type Staphylococcal alpha-hemolysin (alpha-HL) assembly on target mammalian cells usually results in necrotic form of cell death; however, caspase activation also occurs. The pathways of caspase activation due to binding/partial assembly by alpha-HL are unknown ti...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Saumya S Srivastava, Satyabrata Pany, Amita Sneh, Neesar Ahmed, Aejazur Rahman, Krishnasastry V Musti
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a47a6a8880ae4da0be153eea14ba6d5e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a47a6a8880ae4da0be153eea14ba6d5e
record_format	dspace
spelling	oai:doaj.org-article:a47a6a8880ae4da0be153eea14ba6d5e2021-11-25T06:21:31ZMembrane bound monomer of Staphylococcal alpha-hemolysin induces caspase activation and apoptotic cell death despite initiation of membrane repair pathway.1932-620310.1371/journal.pone.0006293https://doaj.org/article/a47a6a8880ae4da0be153eea14ba6d5e2009-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19621082/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Wild type Staphylococcal alpha-hemolysin (alpha-HL) assembly on target mammalian cells usually results in necrotic form of cell death; however, caspase activation also occurs. The pathways of caspase activation due to binding/partial assembly by alpha-HL are unknown till date.<h4>Results</h4>Cells treated with H35N (a mutant of alpha-HL that remains as membrane bound monomer), have been shown to accumulate hypodiploid nuclei, activate caspases and induce intrinsic mitochondrial apoptotic pathway. We have earlier shown that the binding and assembly of alpha-HL requires functional form of Caveolin-1 which is an integral part of caveolae. In this report, we show that the caveolae of mammalian cells, which undergo a continuous cycle of 'kiss and run' dynamics with the plasma membrane, have become immobile upon the binding of the monomer. The cells treated with H35N were unable to recover despite activation of membrane repair mechanism involving caspase-1 dependent activation of sterol regulatory element binding protein-1.<h4>Conclusions</h4>This is for the first time we show the range of cellular changes and responses that take place immediately after the binding of the monomeric form of staphylococcal alpha-hemolysin.Saumya S SrivastavaSatyabrata PanyAmita SnehNeesar AhmedAejazur RahmanKrishnasastry V MustiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 7, p e6293 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Saumya S Srivastava Satyabrata Pany Amita Sneh Neesar Ahmed Aejazur Rahman Krishnasastry V Musti Membrane bound monomer of Staphylococcal alpha-hemolysin induces caspase activation and apoptotic cell death despite initiation of membrane repair pathway.
description	<h4>Background</h4>Wild type Staphylococcal alpha-hemolysin (alpha-HL) assembly on target mammalian cells usually results in necrotic form of cell death; however, caspase activation also occurs. The pathways of caspase activation due to binding/partial assembly by alpha-HL are unknown till date.<h4>Results</h4>Cells treated with H35N (a mutant of alpha-HL that remains as membrane bound monomer), have been shown to accumulate hypodiploid nuclei, activate caspases and induce intrinsic mitochondrial apoptotic pathway. We have earlier shown that the binding and assembly of alpha-HL requires functional form of Caveolin-1 which is an integral part of caveolae. In this report, we show that the caveolae of mammalian cells, which undergo a continuous cycle of 'kiss and run' dynamics with the plasma membrane, have become immobile upon the binding of the monomer. The cells treated with H35N were unable to recover despite activation of membrane repair mechanism involving caspase-1 dependent activation of sterol regulatory element binding protein-1.<h4>Conclusions</h4>This is for the first time we show the range of cellular changes and responses that take place immediately after the binding of the monomeric form of staphylococcal alpha-hemolysin.
format	article
author	Saumya S Srivastava Satyabrata Pany Amita Sneh Neesar Ahmed Aejazur Rahman Krishnasastry V Musti
author_facet	Saumya S Srivastava Satyabrata Pany Amita Sneh Neesar Ahmed Aejazur Rahman Krishnasastry V Musti
author_sort	Saumya S Srivastava
title	Membrane bound monomer of Staphylococcal alpha-hemolysin induces caspase activation and apoptotic cell death despite initiation of membrane repair pathway.
title_short	Membrane bound monomer of Staphylococcal alpha-hemolysin induces caspase activation and apoptotic cell death despite initiation of membrane repair pathway.
title_full	Membrane bound monomer of Staphylococcal alpha-hemolysin induces caspase activation and apoptotic cell death despite initiation of membrane repair pathway.
title_fullStr	Membrane bound monomer of Staphylococcal alpha-hemolysin induces caspase activation and apoptotic cell death despite initiation of membrane repair pathway.
title_full_unstemmed	Membrane bound monomer of Staphylococcal alpha-hemolysin induces caspase activation and apoptotic cell death despite initiation of membrane repair pathway.
title_sort	membrane bound monomer of staphylococcal alpha-hemolysin induces caspase activation and apoptotic cell death despite initiation of membrane repair pathway.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/a47a6a8880ae4da0be153eea14ba6d5e
work_keys_str_mv	AT saumyassrivastava membraneboundmonomerofstaphylococcalalphahemolysininducescaspaseactivationandapoptoticcelldeathdespiteinitiationofmembranerepairpathway AT satyabratapany membraneboundmonomerofstaphylococcalalphahemolysininducescaspaseactivationandapoptoticcelldeathdespiteinitiationofmembranerepairpathway AT amitasneh membraneboundmonomerofstaphylococcalalphahemolysininducescaspaseactivationandapoptoticcelldeathdespiteinitiationofmembranerepairpathway AT neesarahmed membraneboundmonomerofstaphylococcalalphahemolysininducescaspaseactivationandapoptoticcelldeathdespiteinitiationofmembranerepairpathway AT aejazurrahman membraneboundmonomerofstaphylococcalalphahemolysininducescaspaseactivationandapoptoticcelldeathdespiteinitiationofmembranerepairpathway AT krishnasastryvmusti membraneboundmonomerofstaphylococcalalphahemolysininducescaspaseactivationandapoptoticcelldeathdespiteinitiationofmembranerepairpathway
_version_	1718413818542948352

Membrane bound monomer of Staphylococcal alpha-hemolysin induces caspase activation and apoptotic cell death despite initiation of membrane repair pathway.

Ejemplares similares