The Genetic Profile and Serum Level of IL-8 Are Associated with Chronic Hepatitis B and C Virus Infection

The present study evaluated the <i>IL8</i>-251 A/T polymorphism in samples from 74 patients with chronic hepatitis B (HBV), 100 patients with chronic hepatitis C (HCV), and 300 healthy donors (CG). The correlations of this polymorphism with plasma IL-8 and disease stage were calculated....

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Autores principales: Ednelza da Silva Graça Amoras, William Botelho de Brito, Maria Alice Freitas Queiroz, Simone Regina Souza da Silva Conde, Izaura Maria Vieira Cayres Vallinoto, Ricardo Ishak, Antonio Carlos Rosário Vallinoto
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Publicado: MDPI AG 2021
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HBV
HCV
Acceso en línea:https://doaj.org/article/a47b80f0036f47518c40194bd1a8290e
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spelling oai:doaj.org-article:a47b80f0036f47518c40194bd1a8290e2021-11-25T16:53:31ZThe Genetic Profile and Serum Level of IL-8 Are Associated with Chronic Hepatitis B and C Virus Infection10.3390/biom111116642218-273Xhttps://doaj.org/article/a47b80f0036f47518c40194bd1a8290e2021-11-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1664https://doaj.org/toc/2218-273XThe present study evaluated the <i>IL8</i>-251 A/T polymorphism in samples from 74 patients with chronic hepatitis B (HBV), 100 patients with chronic hepatitis C (HCV), and 300 healthy donors (CG). The correlations of this polymorphism with plasma IL-8 and disease stage were calculated. Polymorphisms were identified by real-time PCR. IL-8 was measured by enzyme-linked immunosorbent assay. The <i>IL8</i>-251 A/T genotype was not associated with susceptibility to infection by HBV or HCV. The wild-type allele (A) was associated with higher levels of inflammation (<i>p</i> = 0.0464) and fibrosis scores (<i>p</i> = 0.0016) in the HBV group, representing an increased risk for increased inflammatory activity (<i>OR</i> = 1.84; <i>p</i> = 0.0464) and for high fibrosis scores (<i>OR</i> = 2.63; <i>p</i> = 0.0016). Viral load was higher in HBV patients with polymorphic genotypes (TA and TT) at the <i>IL8</i>-251 A/T polymorphism than in those with the wild-type genotype (<i>p</i> = 0.0272 and <i>p</i> = 0.0464, respectively). Plasma IL-8 was higher among patients infected with HBV or HCV than in the control group (<i>p</i> = 0.0445 and <i>p</i> = 0.0001, respectively). The polymorphic genotype was associated with lower IL-8 than the wild-type genotype in the HBV group (<i>p</i> = 0.0239) and the HCV group (<i>p</i> = 0.0372). The wild-type genotype for <i>IL8</i>-251 A/T and high IL-8 were associated with a worse prognosis for infections; therefore, they may contribute to viral persistence and the development of more severe forms of chronic viral liver diseases.Ednelza da Silva Graça AmorasWilliam Botelho de BritoMaria Alice Freitas QueirozSimone Regina Souza da Silva CondeIzaura Maria Vieira Cayres VallinotoRicardo IshakAntonio Carlos Rosário VallinotoMDPI AGarticleHBVHCVIL-8polymorphismplasma levelsMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1664, p 1664 (2021)
institution DOAJ
collection DOAJ
language EN
topic HBV
HCV
IL-8
polymorphism
plasma levels
Microbiology
QR1-502
spellingShingle HBV
HCV
IL-8
polymorphism
plasma levels
Microbiology
QR1-502
Ednelza da Silva Graça Amoras
William Botelho de Brito
Maria Alice Freitas Queiroz
Simone Regina Souza da Silva Conde
Izaura Maria Vieira Cayres Vallinoto
Ricardo Ishak
Antonio Carlos Rosário Vallinoto
The Genetic Profile and Serum Level of IL-8 Are Associated with Chronic Hepatitis B and C Virus Infection
description The present study evaluated the <i>IL8</i>-251 A/T polymorphism in samples from 74 patients with chronic hepatitis B (HBV), 100 patients with chronic hepatitis C (HCV), and 300 healthy donors (CG). The correlations of this polymorphism with plasma IL-8 and disease stage were calculated. Polymorphisms were identified by real-time PCR. IL-8 was measured by enzyme-linked immunosorbent assay. The <i>IL8</i>-251 A/T genotype was not associated with susceptibility to infection by HBV or HCV. The wild-type allele (A) was associated with higher levels of inflammation (<i>p</i> = 0.0464) and fibrosis scores (<i>p</i> = 0.0016) in the HBV group, representing an increased risk for increased inflammatory activity (<i>OR</i> = 1.84; <i>p</i> = 0.0464) and for high fibrosis scores (<i>OR</i> = 2.63; <i>p</i> = 0.0016). Viral load was higher in HBV patients with polymorphic genotypes (TA and TT) at the <i>IL8</i>-251 A/T polymorphism than in those with the wild-type genotype (<i>p</i> = 0.0272 and <i>p</i> = 0.0464, respectively). Plasma IL-8 was higher among patients infected with HBV or HCV than in the control group (<i>p</i> = 0.0445 and <i>p</i> = 0.0001, respectively). The polymorphic genotype was associated with lower IL-8 than the wild-type genotype in the HBV group (<i>p</i> = 0.0239) and the HCV group (<i>p</i> = 0.0372). The wild-type genotype for <i>IL8</i>-251 A/T and high IL-8 were associated with a worse prognosis for infections; therefore, they may contribute to viral persistence and the development of more severe forms of chronic viral liver diseases.
format article
author Ednelza da Silva Graça Amoras
William Botelho de Brito
Maria Alice Freitas Queiroz
Simone Regina Souza da Silva Conde
Izaura Maria Vieira Cayres Vallinoto
Ricardo Ishak
Antonio Carlos Rosário Vallinoto
author_facet Ednelza da Silva Graça Amoras
William Botelho de Brito
Maria Alice Freitas Queiroz
Simone Regina Souza da Silva Conde
Izaura Maria Vieira Cayres Vallinoto
Ricardo Ishak
Antonio Carlos Rosário Vallinoto
author_sort Ednelza da Silva Graça Amoras
title The Genetic Profile and Serum Level of IL-8 Are Associated with Chronic Hepatitis B and C Virus Infection
title_short The Genetic Profile and Serum Level of IL-8 Are Associated with Chronic Hepatitis B and C Virus Infection
title_full The Genetic Profile and Serum Level of IL-8 Are Associated with Chronic Hepatitis B and C Virus Infection
title_fullStr The Genetic Profile and Serum Level of IL-8 Are Associated with Chronic Hepatitis B and C Virus Infection
title_full_unstemmed The Genetic Profile and Serum Level of IL-8 Are Associated with Chronic Hepatitis B and C Virus Infection
title_sort genetic profile and serum level of il-8 are associated with chronic hepatitis b and c virus infection
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/a47b80f0036f47518c40194bd1a8290e
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