Low temperature plasma irradiation products of sodium lactate solution that induce cell death on U251SP glioblastoma cells were identified

Abstract Low-temperature plasma is being widely used in the various fields of life science, such as medicine and agriculture. Plasma-activated solutions have been proposed as potential cancer therapeutic reagents. We previously reported that plasma-activated Ringer’s lactate solution exhibited selec...

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Autores principales:	Hiromasa Tanaka, Yugo Hosoi, Kenji Ishikawa, Jun Yoshitake, Takahiro Shibata, Koji Uchida, Hiroshi Hashizume, Masaaki Mizuno, Yasumasa Okazaki, Shinya Toyokuni, Kae Nakamura, Hiroaki Kajiyama, Fumitaka Kikkawa, Masaru Hori
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a48ba159ba0b4042bfe8677c7933f572
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spelling	oai:doaj.org-article:a48ba159ba0b4042bfe8677c7933f5722021-12-02T18:02:23ZLow temperature plasma irradiation products of sodium lactate solution that induce cell death on U251SP glioblastoma cells were identified10.1038/s41598-021-98020-w2045-2322https://doaj.org/article/a48ba159ba0b4042bfe8677c7933f5722021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98020-whttps://doaj.org/toc/2045-2322Abstract Low-temperature plasma is being widely used in the various fields of life science, such as medicine and agriculture. Plasma-activated solutions have been proposed as potential cancer therapeutic reagents. We previously reported that plasma-activated Ringer’s lactate solution exhibited selective cancer-killing effects, and that the plasma-treated L-sodium lactate in the solution was an anti-tumor factor; however, the components that are generated through the interactions between plasma and L-sodium lactate and the components responsible for the selective killing of cancer cells remain unidentified. In this study, we quantified several major chemical products, such as pyruvate, formate, and acetate, in plasma-activated L-sodium lactate solution by nuclear magnetic resonance analysis. We further identified novel chemical products, such as glyoxylate and 2,3-dimethyltartrate, in the solution by direct infusion-electrospray ionization with tandem mass spectrometry analysis. We found that 2,3-dimethyltartrate exhibited cytotoxic effects in glioblastoma cells, but not in normal astrocytes. These findings shed light on the identities of the components that are responsible for the selective cytotoxic effect of plasma-activated solutions on cancer cells, and provide useful data for the potential development of cancer treatments using plasma-activated L-sodium lactate solution.Hiromasa TanakaYugo HosoiKenji IshikawaJun YoshitakeTakahiro ShibataKoji UchidaHiroshi HashizumeMasaaki MizunoYasumasa OkazakiShinya ToyokuniKae NakamuraHiroaki KajiyamaFumitaka KikkawaMasaru HoriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hiromasa Tanaka Yugo Hosoi Kenji Ishikawa Jun Yoshitake Takahiro Shibata Koji Uchida Hiroshi Hashizume Masaaki Mizuno Yasumasa Okazaki Shinya Toyokuni Kae Nakamura Hiroaki Kajiyama Fumitaka Kikkawa Masaru Hori Low temperature plasma irradiation products of sodium lactate solution that induce cell death on U251SP glioblastoma cells were identified
description	Abstract Low-temperature plasma is being widely used in the various fields of life science, such as medicine and agriculture. Plasma-activated solutions have been proposed as potential cancer therapeutic reagents. We previously reported that plasma-activated Ringer’s lactate solution exhibited selective cancer-killing effects, and that the plasma-treated L-sodium lactate in the solution was an anti-tumor factor; however, the components that are generated through the interactions between plasma and L-sodium lactate and the components responsible for the selective killing of cancer cells remain unidentified. In this study, we quantified several major chemical products, such as pyruvate, formate, and acetate, in plasma-activated L-sodium lactate solution by nuclear magnetic resonance analysis. We further identified novel chemical products, such as glyoxylate and 2,3-dimethyltartrate, in the solution by direct infusion-electrospray ionization with tandem mass spectrometry analysis. We found that 2,3-dimethyltartrate exhibited cytotoxic effects in glioblastoma cells, but not in normal astrocytes. These findings shed light on the identities of the components that are responsible for the selective cytotoxic effect of plasma-activated solutions on cancer cells, and provide useful data for the potential development of cancer treatments using plasma-activated L-sodium lactate solution.
format	article
author	Hiromasa Tanaka Yugo Hosoi Kenji Ishikawa Jun Yoshitake Takahiro Shibata Koji Uchida Hiroshi Hashizume Masaaki Mizuno Yasumasa Okazaki Shinya Toyokuni Kae Nakamura Hiroaki Kajiyama Fumitaka Kikkawa Masaru Hori
author_facet	Hiromasa Tanaka Yugo Hosoi Kenji Ishikawa Jun Yoshitake Takahiro Shibata Koji Uchida Hiroshi Hashizume Masaaki Mizuno Yasumasa Okazaki Shinya Toyokuni Kae Nakamura Hiroaki Kajiyama Fumitaka Kikkawa Masaru Hori
author_sort	Hiromasa Tanaka
title	Low temperature plasma irradiation products of sodium lactate solution that induce cell death on U251SP glioblastoma cells were identified
title_short	Low temperature plasma irradiation products of sodium lactate solution that induce cell death on U251SP glioblastoma cells were identified
title_full	Low temperature plasma irradiation products of sodium lactate solution that induce cell death on U251SP glioblastoma cells were identified
title_fullStr	Low temperature plasma irradiation products of sodium lactate solution that induce cell death on U251SP glioblastoma cells were identified
title_full_unstemmed	Low temperature plasma irradiation products of sodium lactate solution that induce cell death on U251SP glioblastoma cells were identified
title_sort	low temperature plasma irradiation products of sodium lactate solution that induce cell death on u251sp glioblastoma cells were identified
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a48ba159ba0b4042bfe8677c7933f572
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Low temperature plasma irradiation products of sodium lactate solution that induce cell death on U251SP glioblastoma cells were identified

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