The indispensable contribution of s38 protein to ovarian-eggshell morphogenesis in Drosophila melanogaster
Abstract Drosophila chorion represents a remarkable model system for the in vivo study of complex extracellular-matrix architectures. For its organization and structure, s38 protein is considered as a component of major importance, since it is synthesized and secreted during early choriogenesis. How...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2018
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/a49045d6c5254abab766dd279e5bc670 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:a49045d6c5254abab766dd279e5bc670 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:a49045d6c5254abab766dd279e5bc6702021-12-02T15:08:35ZThe indispensable contribution of s38 protein to ovarian-eggshell morphogenesis in Drosophila melanogaster10.1038/s41598-018-34532-22045-2322https://doaj.org/article/a49045d6c5254abab766dd279e5bc6702018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34532-2https://doaj.org/toc/2045-2322Abstract Drosophila chorion represents a remarkable model system for the in vivo study of complex extracellular-matrix architectures. For its organization and structure, s38 protein is considered as a component of major importance, since it is synthesized and secreted during early choriogenesis. However, there is no evidence that proves its essential, or redundant, role in chorion biogenesis. Hence, we show that targeted downregulation of s38 protein, specifically in the ovarian follicle-cell compartment, via employment of an RNAi-mediated strategy, causes generation of diverse dysmorphic phenotypes, regarding eggshell’s regionally and radially specialized structures. Downregulation of s38 protein severely impairs fly’s fertility and is unable to be compensated by the s36 homologous family member, thus unveiling s38 protein’s essential contribution to chorion’s assembly and function. Altogether, s38 acts as a key skeletal protein being critically implicated in the patterning establishment of a highly structured tripartite endochorion. Furthermore, it seems that s38 loss may sensitize choriogenesis to stochastic variation in its coordination and timing.Athanassios D. VelentzasPanagiotis D. VelentzasStamatia A. KatarachiaAthanasios K. AnagnostopoulosNiki E. SagioglouEleni V. ThanouMaria M. TsiokaVassiliki E. MpakouZoe KolliaVassilios E. GavriilIssidora S. PapassideriGeorge Th. TsangarisAlkiviadis-Constantinos CefalasEvangelia SarantopoulouDimitrios J. StravopodisNature PortfolioarticleEndochorionDorsal AppendagesChorion ProteinsLays EggsAdhesion Force ValuesMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-17 (2018) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Endochorion Dorsal Appendages Chorion Proteins Lays Eggs Adhesion Force Values Medicine R Science Q |
| spellingShingle |
Endochorion Dorsal Appendages Chorion Proteins Lays Eggs Adhesion Force Values Medicine R Science Q Athanassios D. Velentzas Panagiotis D. Velentzas Stamatia A. Katarachia Athanasios K. Anagnostopoulos Niki E. Sagioglou Eleni V. Thanou Maria M. Tsioka Vassiliki E. Mpakou Zoe Kollia Vassilios E. Gavriil Issidora S. Papassideri George Th. Tsangaris Alkiviadis-Constantinos Cefalas Evangelia Sarantopoulou Dimitrios J. Stravopodis The indispensable contribution of s38 protein to ovarian-eggshell morphogenesis in Drosophila melanogaster |
| description |
Abstract Drosophila chorion represents a remarkable model system for the in vivo study of complex extracellular-matrix architectures. For its organization and structure, s38 protein is considered as a component of major importance, since it is synthesized and secreted during early choriogenesis. However, there is no evidence that proves its essential, or redundant, role in chorion biogenesis. Hence, we show that targeted downregulation of s38 protein, specifically in the ovarian follicle-cell compartment, via employment of an RNAi-mediated strategy, causes generation of diverse dysmorphic phenotypes, regarding eggshell’s regionally and radially specialized structures. Downregulation of s38 protein severely impairs fly’s fertility and is unable to be compensated by the s36 homologous family member, thus unveiling s38 protein’s essential contribution to chorion’s assembly and function. Altogether, s38 acts as a key skeletal protein being critically implicated in the patterning establishment of a highly structured tripartite endochorion. Furthermore, it seems that s38 loss may sensitize choriogenesis to stochastic variation in its coordination and timing. |
| format |
article |
| author |
Athanassios D. Velentzas Panagiotis D. Velentzas Stamatia A. Katarachia Athanasios K. Anagnostopoulos Niki E. Sagioglou Eleni V. Thanou Maria M. Tsioka Vassiliki E. Mpakou Zoe Kollia Vassilios E. Gavriil Issidora S. Papassideri George Th. Tsangaris Alkiviadis-Constantinos Cefalas Evangelia Sarantopoulou Dimitrios J. Stravopodis |
| author_facet |
Athanassios D. Velentzas Panagiotis D. Velentzas Stamatia A. Katarachia Athanasios K. Anagnostopoulos Niki E. Sagioglou Eleni V. Thanou Maria M. Tsioka Vassiliki E. Mpakou Zoe Kollia Vassilios E. Gavriil Issidora S. Papassideri George Th. Tsangaris Alkiviadis-Constantinos Cefalas Evangelia Sarantopoulou Dimitrios J. Stravopodis |
| author_sort |
Athanassios D. Velentzas |
| title |
The indispensable contribution of s38 protein to ovarian-eggshell morphogenesis in Drosophila melanogaster |
| title_short |
The indispensable contribution of s38 protein to ovarian-eggshell morphogenesis in Drosophila melanogaster |
| title_full |
The indispensable contribution of s38 protein to ovarian-eggshell morphogenesis in Drosophila melanogaster |
| title_fullStr |
The indispensable contribution of s38 protein to ovarian-eggshell morphogenesis in Drosophila melanogaster |
| title_full_unstemmed |
The indispensable contribution of s38 protein to ovarian-eggshell morphogenesis in Drosophila melanogaster |
| title_sort |
indispensable contribution of s38 protein to ovarian-eggshell morphogenesis in drosophila melanogaster |
| publisher |
Nature Portfolio |
| publishDate |
2018 |
| url |
https://doaj.org/article/a49045d6c5254abab766dd279e5bc670 |
| work_keys_str_mv |
AT athanassiosdvelentzas theindispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT panagiotisdvelentzas theindispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT stamatiaakatarachia theindispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT athanasioskanagnostopoulos theindispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT nikiesagioglou theindispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT elenivthanou theindispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT mariamtsioka theindispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT vassilikiempakou theindispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT zoekollia theindispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT vassiliosegavriil theindispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT issidoraspapassideri theindispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT georgethtsangaris theindispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT alkiviadisconstantinoscefalas theindispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT evangeliasarantopoulou theindispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT dimitriosjstravopodis theindispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT athanassiosdvelentzas indispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT panagiotisdvelentzas indispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT stamatiaakatarachia indispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT athanasioskanagnostopoulos indispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT nikiesagioglou indispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT elenivthanou indispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT mariamtsioka indispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT vassilikiempakou indispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT zoekollia indispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT vassiliosegavriil indispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT issidoraspapassideri indispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT georgethtsangaris indispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT alkiviadisconstantinoscefalas indispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT evangeliasarantopoulou indispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster AT dimitriosjstravopodis indispensablecontributionofs38proteintoovarianeggshellmorphogenesisindrosophilamelanogaster |
| _version_ |
1718388078284898304 |