Protective effects of a standardized extract (HemoHIM) using indomethacin- and ethanol/HCl-induced gastric mucosal injury models

Context: HemoHIM is a medicinal herbal preparation of Angelica gigas Nakai (Apiaceae), Cnidium officinale Makino (Umbelliferae), and Paeonia japonica Miyabe (Paeoniaceae) developed for immune regulation. HemoHIM has been investigated for its ability to enhance tissue self-renewal and stimulate immun...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Da-Ae Kwon, Yong Sang Kim, Sin Hwa Baek, Seul-Ki Kim, Hyun Kyu Kim, Sung-Kee Jo, Uhee Jung, Hae-Ran Park, Hak Sung Lee
Formato: article
Lenguaje:EN
Publicado: Taylor & Francis Group 2019
Materias:
Acceso en línea:https://doaj.org/article/a496a429b6754b1f9a7747d53c3b0742
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:a496a429b6754b1f9a7747d53c3b0742
record_format dspace
spelling oai:doaj.org-article:a496a429b6754b1f9a7747d53c3b07422021-11-17T14:21:56ZProtective effects of a standardized extract (HemoHIM) using indomethacin- and ethanol/HCl-induced gastric mucosal injury models1388-02091744-511610.1080/13880209.2019.1651875https://doaj.org/article/a496a429b6754b1f9a7747d53c3b07422019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1651875https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: HemoHIM is a medicinal herbal preparation of Angelica gigas Nakai (Apiaceae), Cnidium officinale Makino (Umbelliferae), and Paeonia japonica Miyabe (Paeoniaceae) developed for immune regulation. HemoHIM has been investigated for its ability to enhance tissue self-renewal and stimulate immune systems. To date, studies on the protective effects of HemoHIM against gastritis and gastric ulcers have not been conducted. Objective: The protective effects of HemoHIM using models of indomethacin and ethanol/hydrochloric acid (EtOH/HCl)-induced gastric mucosal injury were investigated. Materials and methods: Rats were divided into five groups (n = 10): control, indomethacin, or EtOH/HCl groups, HemoHIM 250, 500 mg kg−1, and cimetidine 100 mg kg−1, respectively. Indomethacin (80 mg kg−1) and 60% EtOH/150 mM HCl were administered orally 1 h after the administration of samples and rats were anesthetized 3 h after induction. The lesion area (%), inhibition ratio (%), and total acidity were investigated, and tissues were histopathologically analyzed using hematoxylin and-eosin (H&E) staining. Results: HemoHIM significantly reduced gastric injury in indomethacin-induced model (250 and 500 mg kg−1; 64.30% and 67.75%, p < 0.001) compared to indomethacin group. In the EtOH/HCl-induced model, HemoHIM reduced gastric lesion (250 and 500 mg kg−1; 61.05% and 73.37%, p < 0.001) and gastric acidity (250 and 500 mg kg−1; 37.80 and 45.20 meq L−1, p < 0.001) compared to EtOH/HCl group. H&E staining of the gastric mucosa showed decreased erosion and hemorrhage in HemoHIM group compared to EtOH/HCl group. Discussion and conclusions: Based on the results, HemoHIM is potential candidate for the treatment of gastritis and gastric ulcers.Da-Ae KwonYong Sang KimSin Hwa BaekSeul-Ki KimHyun Kyu KimSung-Kee JoUhee JungHae-Ran ParkHak Sung LeeTaylor & Francis Grouparticleinflammationgastritisgastric ulcerangelica gigas nakaicnidium officinale makinopaeonia japonica miyabeTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 543-549 (2019)
institution DOAJ
collection DOAJ
language EN
topic inflammation
gastritis
gastric ulcer
angelica gigas nakai
cnidium officinale makino
paeonia japonica miyabe
Therapeutics. Pharmacology
RM1-950
spellingShingle inflammation
gastritis
gastric ulcer
angelica gigas nakai
cnidium officinale makino
paeonia japonica miyabe
Therapeutics. Pharmacology
RM1-950
Da-Ae Kwon
Yong Sang Kim
Sin Hwa Baek
Seul-Ki Kim
Hyun Kyu Kim
Sung-Kee Jo
Uhee Jung
Hae-Ran Park
Hak Sung Lee
Protective effects of a standardized extract (HemoHIM) using indomethacin- and ethanol/HCl-induced gastric mucosal injury models
description Context: HemoHIM is a medicinal herbal preparation of Angelica gigas Nakai (Apiaceae), Cnidium officinale Makino (Umbelliferae), and Paeonia japonica Miyabe (Paeoniaceae) developed for immune regulation. HemoHIM has been investigated for its ability to enhance tissue self-renewal and stimulate immune systems. To date, studies on the protective effects of HemoHIM against gastritis and gastric ulcers have not been conducted. Objective: The protective effects of HemoHIM using models of indomethacin and ethanol/hydrochloric acid (EtOH/HCl)-induced gastric mucosal injury were investigated. Materials and methods: Rats were divided into five groups (n = 10): control, indomethacin, or EtOH/HCl groups, HemoHIM 250, 500 mg kg−1, and cimetidine 100 mg kg−1, respectively. Indomethacin (80 mg kg−1) and 60% EtOH/150 mM HCl were administered orally 1 h after the administration of samples and rats were anesthetized 3 h after induction. The lesion area (%), inhibition ratio (%), and total acidity were investigated, and tissues were histopathologically analyzed using hematoxylin and-eosin (H&E) staining. Results: HemoHIM significantly reduced gastric injury in indomethacin-induced model (250 and 500 mg kg−1; 64.30% and 67.75%, p < 0.001) compared to indomethacin group. In the EtOH/HCl-induced model, HemoHIM reduced gastric lesion (250 and 500 mg kg−1; 61.05% and 73.37%, p < 0.001) and gastric acidity (250 and 500 mg kg−1; 37.80 and 45.20 meq L−1, p < 0.001) compared to EtOH/HCl group. H&E staining of the gastric mucosa showed decreased erosion and hemorrhage in HemoHIM group compared to EtOH/HCl group. Discussion and conclusions: Based on the results, HemoHIM is potential candidate for the treatment of gastritis and gastric ulcers.
format article
author Da-Ae Kwon
Yong Sang Kim
Sin Hwa Baek
Seul-Ki Kim
Hyun Kyu Kim
Sung-Kee Jo
Uhee Jung
Hae-Ran Park
Hak Sung Lee
author_facet Da-Ae Kwon
Yong Sang Kim
Sin Hwa Baek
Seul-Ki Kim
Hyun Kyu Kim
Sung-Kee Jo
Uhee Jung
Hae-Ran Park
Hak Sung Lee
author_sort Da-Ae Kwon
title Protective effects of a standardized extract (HemoHIM) using indomethacin- and ethanol/HCl-induced gastric mucosal injury models
title_short Protective effects of a standardized extract (HemoHIM) using indomethacin- and ethanol/HCl-induced gastric mucosal injury models
title_full Protective effects of a standardized extract (HemoHIM) using indomethacin- and ethanol/HCl-induced gastric mucosal injury models
title_fullStr Protective effects of a standardized extract (HemoHIM) using indomethacin- and ethanol/HCl-induced gastric mucosal injury models
title_full_unstemmed Protective effects of a standardized extract (HemoHIM) using indomethacin- and ethanol/HCl-induced gastric mucosal injury models
title_sort protective effects of a standardized extract (hemohim) using indomethacin- and ethanol/hcl-induced gastric mucosal injury models
publisher Taylor & Francis Group
publishDate 2019
url https://doaj.org/article/a496a429b6754b1f9a7747d53c3b0742
work_keys_str_mv AT daaekwon protectiveeffectsofastandardizedextracthemohimusingindomethacinandethanolhclinducedgastricmucosalinjurymodels
AT yongsangkim protectiveeffectsofastandardizedextracthemohimusingindomethacinandethanolhclinducedgastricmucosalinjurymodels
AT sinhwabaek protectiveeffectsofastandardizedextracthemohimusingindomethacinandethanolhclinducedgastricmucosalinjurymodels
AT seulkikim protectiveeffectsofastandardizedextracthemohimusingindomethacinandethanolhclinducedgastricmucosalinjurymodels
AT hyunkyukim protectiveeffectsofastandardizedextracthemohimusingindomethacinandethanolhclinducedgastricmucosalinjurymodels
AT sungkeejo protectiveeffectsofastandardizedextracthemohimusingindomethacinandethanolhclinducedgastricmucosalinjurymodels
AT uheejung protectiveeffectsofastandardizedextracthemohimusingindomethacinandethanolhclinducedgastricmucosalinjurymodels
AT haeranpark protectiveeffectsofastandardizedextracthemohimusingindomethacinandethanolhclinducedgastricmucosalinjurymodels
AT haksunglee protectiveeffectsofastandardizedextracthemohimusingindomethacinandethanolhclinducedgastricmucosalinjurymodels
_version_ 1718425500403105792