A Comprehensive Genome-wide Analysis of Long Non-coding RNA and mRNA Expression Profiles of JAK2V617F-positive Classical Myeloproliferative Neoplasms

Aberrant expression of long non-coding RNAs (lncRNAs) is involved in the progression of myeloid neoplasms, but the role of lncRNAs in the JAK2V617F-positive subtype of classical myeloproliferative neoplasms (cMPNs) remains unclear. This study was conducted to clarify the expression and regulation pa...

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Autores principales:	Jie Zhou, Hao Wu, Cheng Guo, Bing Li, Li-Li Zhou, Ai-Bin Liang, Jian-Fei Fu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	classical myeloproliferative neoplasms jak2v617f-positive lncrna mrna microarray analysis itgb3 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/a49dad70d7b94da89b20c34b0e2a04f3
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spelling	oai:doaj.org-article:a49dad70d7b94da89b20c34b0e2a04f32021-11-11T14:23:43ZA Comprehensive Genome-wide Analysis of Long Non-coding RNA and mRNA Expression Profiles of JAK2V617F-positive Classical Myeloproliferative Neoplasms2165-59792165-598710.1080/21655979.2021.2000226https://doaj.org/article/a49dad70d7b94da89b20c34b0e2a04f32021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2000226https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Aberrant expression of long non-coding RNAs (lncRNAs) is involved in the progression of myeloid neoplasms, but the role of lncRNAs in the JAK2V617F-positive subtype of classical myeloproliferative neoplasms (cMPNs) remains unclear. This study was conducted to clarify the expression and regulation patterns of lncRNAs in JAK2V617F-positive cMPNs, and to explore new potential carcinogenic factors of cMPNs. Bioinformatics analysis of microarray detection and wet testing verification were performed to study the expression and regulation signature of differentially expressed lncRNAs (DELs) and related genes (DEGs) in cMPNs. The expression of lncRNAs and mRNAs were observed to significantly dysregulated in JAK2V617F-positive cMPN patients compared with the normal controls. Co-expression analysis indicated that there were significant differences of the co-expression pattern of lncRNAs and mRNAs in JAK2V617F-positive cMPN patients compared to normal controls. GO and KEGG pathway analysis of DEGs and DELs showed the involvement of several pathways previously reported to regulate the pathogenesis of leukemia and cMPNs. Cis- and trans-regulation analysis of lncRNAs showed that ZNF141, DHX29, NOC2L, MAS1L, AFAP1L1, and CPN2 were significantly cis-regulated by lncRNA ENST00000356347, ENST00000456816, hsa-mir-449c, NR_026874, TCONS_00012136, uc003lqp.2, and ENST00000456816, respectively, and DELs were mostly correlated with transcription factors including CTBP2, SUZ12, REST, STAT2, and GATA4 to jointly regulate multiple target genes. In summary, expression profiles of lncRNAs and mRNAs were significantly altered in JAK2V617F-positive cMPNs, the relative signaling pathway, co-expression, cis- and trans-regulation were regulated by dysregulation of lncRNAs and several important genes, such as ITGB3, which may act as a promising carcinogenic factor, warrant further investigation.Jie ZhouHao WuCheng GuoBing LiLi-Li ZhouAi-Bin LiangJian-Fei FuTaylor & Francis Grouparticleclassical myeloproliferative neoplasmsjak2v617f-positivelncrnamrnamicroarray analysisitgb3BiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	classical myeloproliferative neoplasms jak2v617f-positive lncrna mrna microarray analysis itgb3 Biotechnology TP248.13-248.65
spellingShingle	classical myeloproliferative neoplasms jak2v617f-positive lncrna mrna microarray analysis itgb3 Biotechnology TP248.13-248.65 Jie Zhou Hao Wu Cheng Guo Bing Li Li-Li Zhou Ai-Bin Liang Jian-Fei Fu A Comprehensive Genome-wide Analysis of Long Non-coding RNA and mRNA Expression Profiles of JAK2V617F-positive Classical Myeloproliferative Neoplasms
description	Aberrant expression of long non-coding RNAs (lncRNAs) is involved in the progression of myeloid neoplasms, but the role of lncRNAs in the JAK2V617F-positive subtype of classical myeloproliferative neoplasms (cMPNs) remains unclear. This study was conducted to clarify the expression and regulation patterns of lncRNAs in JAK2V617F-positive cMPNs, and to explore new potential carcinogenic factors of cMPNs. Bioinformatics analysis of microarray detection and wet testing verification were performed to study the expression and regulation signature of differentially expressed lncRNAs (DELs) and related genes (DEGs) in cMPNs. The expression of lncRNAs and mRNAs were observed to significantly dysregulated in JAK2V617F-positive cMPN patients compared with the normal controls. Co-expression analysis indicated that there were significant differences of the co-expression pattern of lncRNAs and mRNAs in JAK2V617F-positive cMPN patients compared to normal controls. GO and KEGG pathway analysis of DEGs and DELs showed the involvement of several pathways previously reported to regulate the pathogenesis of leukemia and cMPNs. Cis- and trans-regulation analysis of lncRNAs showed that ZNF141, DHX29, NOC2L, MAS1L, AFAP1L1, and CPN2 were significantly cis-regulated by lncRNA ENST00000356347, ENST00000456816, hsa-mir-449c, NR_026874, TCONS_00012136, uc003lqp.2, and ENST00000456816, respectively, and DELs were mostly correlated with transcription factors including CTBP2, SUZ12, REST, STAT2, and GATA4 to jointly regulate multiple target genes. In summary, expression profiles of lncRNAs and mRNAs were significantly altered in JAK2V617F-positive cMPNs, the relative signaling pathway, co-expression, cis- and trans-regulation were regulated by dysregulation of lncRNAs and several important genes, such as ITGB3, which may act as a promising carcinogenic factor, warrant further investigation.
format	article
author	Jie Zhou Hao Wu Cheng Guo Bing Li Li-Li Zhou Ai-Bin Liang Jian-Fei Fu
author_facet	Jie Zhou Hao Wu Cheng Guo Bing Li Li-Li Zhou Ai-Bin Liang Jian-Fei Fu
author_sort	Jie Zhou
title	A Comprehensive Genome-wide Analysis of Long Non-coding RNA and mRNA Expression Profiles of JAK2V617F-positive Classical Myeloproliferative Neoplasms
title_short	A Comprehensive Genome-wide Analysis of Long Non-coding RNA and mRNA Expression Profiles of JAK2V617F-positive Classical Myeloproliferative Neoplasms
title_full	A Comprehensive Genome-wide Analysis of Long Non-coding RNA and mRNA Expression Profiles of JAK2V617F-positive Classical Myeloproliferative Neoplasms
title_fullStr	A Comprehensive Genome-wide Analysis of Long Non-coding RNA and mRNA Expression Profiles of JAK2V617F-positive Classical Myeloproliferative Neoplasms
title_full_unstemmed	A Comprehensive Genome-wide Analysis of Long Non-coding RNA and mRNA Expression Profiles of JAK2V617F-positive Classical Myeloproliferative Neoplasms
title_sort	comprehensive genome-wide analysis of long non-coding rna and mrna expression profiles of jak2v617f-positive classical myeloproliferative neoplasms
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/a49dad70d7b94da89b20c34b0e2a04f3
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A Comprehensive Genome-wide Analysis of Long Non-coding RNA and mRNA Expression Profiles of JAK2V617F-positive Classical Myeloproliferative Neoplasms

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