Protective role of trehalose during radiation and heavy metal stress in Aureobasidium subglaciale F134

Abstract An isolated black yeast-like strain was obtained from radiation-polluted soil collected from Xinjiang province in northwest China. On the basis of ITS and LSU rDNA sequence analysis, in combination with the colony morphology and phenotypic properties, the isolated strain was revealed to rep...

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Autores principales:	Tingting Liu, Liying Zhu, Zhiping Zhang, He Huang, Zhidong Zhang, Ling Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a4a2b96324be4a37ada88104874c2e65
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spelling	oai:doaj.org-article:a4a2b96324be4a37ada88104874c2e652021-12-02T15:05:02ZProtective role of trehalose during radiation and heavy metal stress in Aureobasidium subglaciale F13410.1038/s41598-017-15489-02045-2322https://doaj.org/article/a4a2b96324be4a37ada88104874c2e652017-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-15489-0https://doaj.org/toc/2045-2322Abstract An isolated black yeast-like strain was obtained from radiation-polluted soil collected from Xinjiang province in northwest China. On the basis of ITS and LSU rDNA sequence analysis, in combination with the colony morphology and phenotypic properties, the isolated strain was revealed to represent a novel variety of Aureobasidium subglaciale, designated as A. subglaciale F134. Compared to other yeasts and bacteria, this isolate displayed superior resistance to gamma irradiation, UV light, and heavy metal ions. It was discovered that the resistance of the isolate was correlated with the stress protector trehalose. Through the overexpression of the trehalose-6-phosphate synthase gene tps1 and the deletion of acid trehalase gene ath1, the APT∆A double mutant exhibited a survival rate of 1% under 20 kGy of gamma-radiation, 2% survival rate at a UV dosage of 250 J/m2, and tolerance towards Pb2+ as high as 1500 mg/L, which was in agreement with the high accumulation of intracellular trehalose compared to the wild-type strain. Finally, the protective effects and the mechanism of trehalose accumulation in A. subglaciale F134 were investigated, revealing a significant activation of the expression of many of the stress tolerance genes, offering new perspectives on the adaptations of radioresistant microorganisms.Tingting LiuLiying ZhuZhiping ZhangHe HuangZhidong ZhangLing JiangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tingting Liu Liying Zhu Zhiping Zhang He Huang Zhidong Zhang Ling Jiang Protective role of trehalose during radiation and heavy metal stress in Aureobasidium subglaciale F134
description	Abstract An isolated black yeast-like strain was obtained from radiation-polluted soil collected from Xinjiang province in northwest China. On the basis of ITS and LSU rDNA sequence analysis, in combination with the colony morphology and phenotypic properties, the isolated strain was revealed to represent a novel variety of Aureobasidium subglaciale, designated as A. subglaciale F134. Compared to other yeasts and bacteria, this isolate displayed superior resistance to gamma irradiation, UV light, and heavy metal ions. It was discovered that the resistance of the isolate was correlated with the stress protector trehalose. Through the overexpression of the trehalose-6-phosphate synthase gene tps1 and the deletion of acid trehalase gene ath1, the APT∆A double mutant exhibited a survival rate of 1% under 20 kGy of gamma-radiation, 2% survival rate at a UV dosage of 250 J/m2, and tolerance towards Pb2+ as high as 1500 mg/L, which was in agreement with the high accumulation of intracellular trehalose compared to the wild-type strain. Finally, the protective effects and the mechanism of trehalose accumulation in A. subglaciale F134 were investigated, revealing a significant activation of the expression of many of the stress tolerance genes, offering new perspectives on the adaptations of radioresistant microorganisms.
format	article
author	Tingting Liu Liying Zhu Zhiping Zhang He Huang Zhidong Zhang Ling Jiang
author_facet	Tingting Liu Liying Zhu Zhiping Zhang He Huang Zhidong Zhang Ling Jiang
author_sort	Tingting Liu
title	Protective role of trehalose during radiation and heavy metal stress in Aureobasidium subglaciale F134
title_short	Protective role of trehalose during radiation and heavy metal stress in Aureobasidium subglaciale F134
title_full	Protective role of trehalose during radiation and heavy metal stress in Aureobasidium subglaciale F134
title_fullStr	Protective role of trehalose during radiation and heavy metal stress in Aureobasidium subglaciale F134
title_full_unstemmed	Protective role of trehalose during radiation and heavy metal stress in Aureobasidium subglaciale F134
title_sort	protective role of trehalose during radiation and heavy metal stress in aureobasidium subglaciale f134
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a4a2b96324be4a37ada88104874c2e65
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Protective role of trehalose during radiation and heavy metal stress in Aureobasidium subglaciale F134

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