Sorghum extract: Phytochemical, proximate, and GC-MS analyses

Introduction. Sorghum is available cereal seeds of African origin belonging to the Poaceae family. However, its metabolites and proximate composition have not studied well, which led to the under-utilization of this cereal. This research aimed to investigate the classes of phytochemical and proximat...

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Autores principales:	Olayinka O. Ajani, Taiwo F. Owoeye, Kehinde D. Akinlabu, Oladotun P. Bolade, Oluwatimilehin E. Aribisala, Bamidele M. Durodola
Formato:	article
Lenguaje:	EN
Publicado:	Kemerovo State University 2021
Materias:	sorghum phytochemicals nutritional value chromatography proximate analysis bioactive compounds grain Food processing and manufacture TP368-456
Acceso en línea:	https://doaj.org/article/a4a8b3d468a94e5ebdc0295afcde3a09
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spelling	oai:doaj.org-article:a4a8b3d468a94e5ebdc0295afcde3a092021-11-19T04:02:56ZSorghum extract: Phytochemical, proximate, and GC-MS analyses2308-40572310-959910.21603/2308-4057-2021-2-371-378https://doaj.org/article/a4a8b3d468a94e5ebdc0295afcde3a092021-11-01T00:00:00Zhttp://jfrm.ru/en/issues/1879/1951/https://doaj.org/toc/2308-4057https://doaj.org/toc/2310-9599Introduction. Sorghum is available cereal seeds of African origin belonging to the Poaceae family. However, its metabolites and proximate composition have not studied well, which led to the under-utilization of this cereal. This research aimed to investigate the classes of phytochemical and proximate compositions of sorghum extract in order to assess its nutraceutical potential for food chemistry and dietary formulations. Study objects and methods. We studied the sorghum seed oil extract obtained with the help of a Soxhlet extractor. Sorghum was purchased in Ota, Nigeria. The bioactive compounds were identified by standard methods of phytochemical screening, the nutritional content was investigated with proximate analysis, and the secondary metabolites in the sorghum extract were determined using gas chromatography – mass spectrometry (GC-MS). Result and discussion. The phytochemical screening showed the presence of steroids, saponins, terpenoids, alkaloids, cardiac glycosides, and quinones in the sorghum extract. The oil yield obtained was 11.00 ± 0.18%. The proximate analysis revealed 5.94% moisture content, 3.05% ash, 0.20% crude fiber, 11.00% fat, 5.54% protein, and 74.27% carbohydrates. The selected physicochemical parameters measured in the sorghum extract included cloud point (0.40°C), specific gravity at 25°C (0.81), and refractive index (1.46). The GC-MS analysis revealed the presence of 9,12-octadecadienoic acid (Z,Z)-, stigmasterol, 8-dodecen-1-ol, acetate, (Z)-, vitamin E, linoleic acid ethyl ester, and 9,12-octadecadienoic acid, methyl ester, which accounted for about 85% in the sorghum composition. Other constituents, presented at lower amounts, included 12-heptadecyn-1-ol, 1H-Imidazole-5-ethanamine, 1-methyl-, and cyclononene. Conclusion. The findings of this study revealed high nutritive potential of sorghum, which make it a rich source of energy for humans and animals.Olayinka O. AjaniTaiwo F. OwoeyeKehinde D. AkinlabuOladotun P. BoladeOluwatimilehin E. AribisalaBamidele M. DurodolaKemerovo State Universityarticlesorghumphytochemicalsnutritional valuechromatographyproximate analysisbioactive compoundsgrainFood processing and manufactureTP368-456ENFoods and Raw Materials, Vol 9, Iss 2, Pp 371-378 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sorghum phytochemicals nutritional value chromatography proximate analysis bioactive compounds grain Food processing and manufacture TP368-456
spellingShingle	sorghum phytochemicals nutritional value chromatography proximate analysis bioactive compounds grain Food processing and manufacture TP368-456 Olayinka O. Ajani Taiwo F. Owoeye Kehinde D. Akinlabu Oladotun P. Bolade Oluwatimilehin E. Aribisala Bamidele M. Durodola Sorghum extract: Phytochemical, proximate, and GC-MS analyses
description	Introduction. Sorghum is available cereal seeds of African origin belonging to the Poaceae family. However, its metabolites and proximate composition have not studied well, which led to the under-utilization of this cereal. This research aimed to investigate the classes of phytochemical and proximate compositions of sorghum extract in order to assess its nutraceutical potential for food chemistry and dietary formulations. Study objects and methods. We studied the sorghum seed oil extract obtained with the help of a Soxhlet extractor. Sorghum was purchased in Ota, Nigeria. The bioactive compounds were identified by standard methods of phytochemical screening, the nutritional content was investigated with proximate analysis, and the secondary metabolites in the sorghum extract were determined using gas chromatography – mass spectrometry (GC-MS). Result and discussion. The phytochemical screening showed the presence of steroids, saponins, terpenoids, alkaloids, cardiac glycosides, and quinones in the sorghum extract. The oil yield obtained was 11.00 ± 0.18%. The proximate analysis revealed 5.94% moisture content, 3.05% ash, 0.20% crude fiber, 11.00% fat, 5.54% protein, and 74.27% carbohydrates. The selected physicochemical parameters measured in the sorghum extract included cloud point (0.40°C), specific gravity at 25°C (0.81), and refractive index (1.46). The GC-MS analysis revealed the presence of 9,12-octadecadienoic acid (Z,Z)-, stigmasterol, 8-dodecen-1-ol, acetate, (Z)-, vitamin E, linoleic acid ethyl ester, and 9,12-octadecadienoic acid, methyl ester, which accounted for about 85% in the sorghum composition. Other constituents, presented at lower amounts, included 12-heptadecyn-1-ol, 1H-Imidazole-5-ethanamine, 1-methyl-, and cyclononene. Conclusion. The findings of this study revealed high nutritive potential of sorghum, which make it a rich source of energy for humans and animals.
format	article
author	Olayinka O. Ajani Taiwo F. Owoeye Kehinde D. Akinlabu Oladotun P. Bolade Oluwatimilehin E. Aribisala Bamidele M. Durodola
author_facet	Olayinka O. Ajani Taiwo F. Owoeye Kehinde D. Akinlabu Oladotun P. Bolade Oluwatimilehin E. Aribisala Bamidele M. Durodola
author_sort	Olayinka O. Ajani
title	Sorghum extract: Phytochemical, proximate, and GC-MS analyses
title_short	Sorghum extract: Phytochemical, proximate, and GC-MS analyses
title_full	Sorghum extract: Phytochemical, proximate, and GC-MS analyses
title_fullStr	Sorghum extract: Phytochemical, proximate, and GC-MS analyses
title_full_unstemmed	Sorghum extract: Phytochemical, proximate, and GC-MS analyses
title_sort	sorghum extract: phytochemical, proximate, and gc-ms analyses
publisher	Kemerovo State University
publishDate	2021
url	https://doaj.org/article/a4a8b3d468a94e5ebdc0295afcde3a09
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Sorghum extract: Phytochemical, proximate, and GC-MS analyses

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