Peri-Prostatic Adipocyte-Released TGFβ Enhances Prostate Cancer Cell Motility by Upregulation of Connective Tissue Growth Factor

Periprostatic adipose tissue (PPAT) has emerged as a key player in the prostate cancer (PCa) microenvironment. In this study, we evaluated the ability of PPAT to promote PCa cell migration, as well as the molecular mechanisms involved. Methods: We collected conditioned mediums from in vitro differen...

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Autores principales:	Evelina La Civita, Antonietta Liotti, Michele Cennamo, Felice Crocetto, Matteo Ferro, Pasquale Liguoro, Amelia Cimmino, Ciro Imbimbo, Francesco Beguinot, Pietro Formisano, Daniela Terracciano
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	adipocytes prostate cancer TGFβ1 peri-prostatic adipose tissue cell migration Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a4a980bb92f74dc9babe0bac00f3cabd
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spelling	oai:doaj.org-article:a4a980bb92f74dc9babe0bac00f3cabd2021-11-25T16:50:54ZPeri-Prostatic Adipocyte-Released TGFβ Enhances Prostate Cancer Cell Motility by Upregulation of Connective Tissue Growth Factor10.3390/biomedicines91116922227-9059https://doaj.org/article/a4a980bb92f74dc9babe0bac00f3cabd2021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1692https://doaj.org/toc/2227-9059Periprostatic adipose tissue (PPAT) has emerged as a key player in the prostate cancer (PCa) microenvironment. In this study, we evaluated the ability of PPAT to promote PCa cell migration, as well as the molecular mechanisms involved. Methods: We collected conditioned mediums from in vitro differentiated adipocytes isolated from PPAT taken from PCa patients during radical prostatectomy. Migration was studied by scratch assay. Results: Culture with CM of human PPAT (AdipoCM) promotes migration in two different human androgen-independent (AI) PCa cell lines (DU145 and PC3) and upregulated the expression of CTGF. SB431542, a well-known TGFβ receptor inhibitor, counteracts the increased migration observed in presence of AdipoCM and decreased CTGF expression, suggesting that a paracrine secretion of TGFβ by PPAT affects motility of PCa cells. Conclusions: Collectively, our study showed that factors secreted by PPAT enhanced migration through CTGF upregulation in AI PCa cell lines. These findings reveal the potential of novel therapeutic strategies targeting adipocyte-released factors and TGFβ/CTGF axis to fight advanced PCa dissemination.Evelina La CivitaAntonietta LiottiMichele CennamoFelice CrocettoMatteo FerroPasquale LiguoroAmelia CimminoCiro ImbimboFrancesco BeguinotPietro FormisanoDaniela TerraccianoMDPI AGarticleadipocytesprostate cancerTGFβ1peri-prostatic adipose tissuecell migrationBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1692, p 1692 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adipocytes prostate cancer TGFβ1 peri-prostatic adipose tissue cell migration Biology (General) QH301-705.5
spellingShingle	adipocytes prostate cancer TGFβ1 peri-prostatic adipose tissue cell migration Biology (General) QH301-705.5 Evelina La Civita Antonietta Liotti Michele Cennamo Felice Crocetto Matteo Ferro Pasquale Liguoro Amelia Cimmino Ciro Imbimbo Francesco Beguinot Pietro Formisano Daniela Terracciano Peri-Prostatic Adipocyte-Released TGFβ Enhances Prostate Cancer Cell Motility by Upregulation of Connective Tissue Growth Factor
description	Periprostatic adipose tissue (PPAT) has emerged as a key player in the prostate cancer (PCa) microenvironment. In this study, we evaluated the ability of PPAT to promote PCa cell migration, as well as the molecular mechanisms involved. Methods: We collected conditioned mediums from in vitro differentiated adipocytes isolated from PPAT taken from PCa patients during radical prostatectomy. Migration was studied by scratch assay. Results: Culture with CM of human PPAT (AdipoCM) promotes migration in two different human androgen-independent (AI) PCa cell lines (DU145 and PC3) and upregulated the expression of CTGF. SB431542, a well-known TGFβ receptor inhibitor, counteracts the increased migration observed in presence of AdipoCM and decreased CTGF expression, suggesting that a paracrine secretion of TGFβ by PPAT affects motility of PCa cells. Conclusions: Collectively, our study showed that factors secreted by PPAT enhanced migration through CTGF upregulation in AI PCa cell lines. These findings reveal the potential of novel therapeutic strategies targeting adipocyte-released factors and TGFβ/CTGF axis to fight advanced PCa dissemination.
format	article
author	Evelina La Civita Antonietta Liotti Michele Cennamo Felice Crocetto Matteo Ferro Pasquale Liguoro Amelia Cimmino Ciro Imbimbo Francesco Beguinot Pietro Formisano Daniela Terracciano
author_facet	Evelina La Civita Antonietta Liotti Michele Cennamo Felice Crocetto Matteo Ferro Pasquale Liguoro Amelia Cimmino Ciro Imbimbo Francesco Beguinot Pietro Formisano Daniela Terracciano
author_sort	Evelina La Civita
title	Peri-Prostatic Adipocyte-Released TGFβ Enhances Prostate Cancer Cell Motility by Upregulation of Connective Tissue Growth Factor
title_short	Peri-Prostatic Adipocyte-Released TGFβ Enhances Prostate Cancer Cell Motility by Upregulation of Connective Tissue Growth Factor
title_full	Peri-Prostatic Adipocyte-Released TGFβ Enhances Prostate Cancer Cell Motility by Upregulation of Connective Tissue Growth Factor
title_fullStr	Peri-Prostatic Adipocyte-Released TGFβ Enhances Prostate Cancer Cell Motility by Upregulation of Connective Tissue Growth Factor
title_full_unstemmed	Peri-Prostatic Adipocyte-Released TGFβ Enhances Prostate Cancer Cell Motility by Upregulation of Connective Tissue Growth Factor
title_sort	peri-prostatic adipocyte-released tgfβ enhances prostate cancer cell motility by upregulation of connective tissue growth factor
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a4a980bb92f74dc9babe0bac00f3cabd
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Peri-Prostatic Adipocyte-Released TGFβ Enhances Prostate Cancer Cell Motility by Upregulation of Connective Tissue Growth Factor

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