Antimicrobial and antibiofilm activity of biopolymer-Ni, Zn nanoparticle biocomposites synthesized using R. mucilaginosa UANL-001L exopolysaccharide as a capping agent

Javier Alberto Garza-Cervantes,1,2 C Enrique Escárcega-González,1–3 E Díaz Barriga Castro,4 G Mendiola-Garza,1,2 Bruno Antonio Marichal-Cancino,3 Mario Alberto López-Vázquez,3 Jose Ruben Morones-Ramirez1,21Universidad Autóno...

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Autores principales:	Garza-Cervantes JA, Escárcega-González CE, Díaz Barriga Castro E, Mendiola-Garza G, Marichal-Cancino BA, López-Vázquez MA, Morones-Ramirez JR
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Nickel Zinc Nanoparticles Antibiofilm activity Exopolysaccharide capping agent Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a4acfee33a0b49dfa835994f958624c4
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record_format	dspace
institution	DOAJ
collection	DOAJ
language	EN
topic	Nickel Zinc Nanoparticles Antibiofilm activity Exopolysaccharide capping agent Medicine (General) R5-920
spellingShingle	Nickel Zinc Nanoparticles Antibiofilm activity Exopolysaccharide capping agent Medicine (General) R5-920 Garza-Cervantes JA Escárcega-González CE Díaz Barriga Castro E Mendiola-Garza G Marichal-Cancino BA López-Vázquez MA Morones-Ramirez JR Antimicrobial and antibiofilm activity of biopolymer-Ni, Zn nanoparticle biocomposites synthesized using R. mucilaginosa UANL-001L exopolysaccharide as a capping agent
description	Javier Alberto Garza-Cervantes,1,2 C Enrique Escárcega-González,1–3 E Díaz Barriga Castro,4 G Mendiola-Garza,1,2 Bruno Antonio Marichal-Cancino,3 Mario Alberto López-Vázquez,3 Jose Ruben Morones-Ramirez1,21Universidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Químicas, San Nicolás de los Garza, NL 66451, México; 2Centro de Investigación en Biotecnología y Nanotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, Parque de Investigación e Innovación Tecnológica, Apodaca 66629, Nuevo León, México; 3Universidad Autónoma de Aguascalientes, Departamento de Fisiología y Farmacología, Centro de Ciencias Básicas, Aguascalientes, Mexico; 4Centro de Investigación en Química Aplicada, Saltillo, Coahuila, MéxicoIntroduction: Global increase in the consumption of antibiotics has induced selective stress on wild-type microorganisms, pushing them to adapt to conditions of higher antibiotic concentrations, and thus an increased variety of resistant bacterial strains have emerged. Metal nanoparticles synthesized by green methods have been studied and proposed as potential antimicrobial agents against both wild-type and antibiotic-resistant strains; in addition, exopolysaccharides have been used as capping agent of metal nanoparticles due to their biocompatibility, reducing biological risks in a wide variety of applications.Purpose: In this work, we use an exopolysaccharide, from Rhodotorula mucilaginosa UANL-001L, an autochthonous strain from the Mexican northeast, as a capping agent in the synthesis of Zn, and Ni, nanoparticle biopolymer biocomposites.Materials and methods: To physically and chemically characterize the synthesized biocomposites, FT-IR, UV-Vs, TEM, SAED and EDS analysis were carried out. Antimicrobial and antibiofilm biological activity were tested for the biocomposites against two resistant clinical strains, a Gram-positive Staphylococcus aureus, and a Gram-negative Pseudomonas aeruginosa. Antimicrobial activity was determined using a microdilution assay whereas antibiofilm activity was analyzed through crystal violet staining.Results: Biocomposites composed of exopolysaccharide capped Zn and Ni metal nanoparticles were synthesized through a green synthesis methodology. The average size of the Zn and Ni nanoparticles ranged between 8 and 26 nm, respectively. The Ni-EPS biocomposites showed antimicrobial and antibiofilm activity against resistant strains of Staphylococcus aureus and Pseudomonas aeruginosa at 3 and 2 mg/mL, respectively. Moreover, Zn-EPS biocomposites showed antimicrobial activity against resistant Staphylococcus aureus at 1 mg/mL. Both biocomposites showed no toxicity, as renal function showed no differences between treatments and control in the in vivo assays with male rats tests in this study at a concentration of 24 mg/kg of body weight.Conclusion: The exopolysaccharide produced by Rhodotorula mucilaginosa UANL-001L is an excellent candidate as a capping agent in the synthesis of biopolymer-metal nanoparticle biocomposites. Both Ni and Zn-EPS biocomposites demonstrate to be potential contenders as novel antimicrobial agents against both Gram-negative and Gram-positive clinically relevant resistant bacterial strains. Moreover, Ni-EPS biocomposites also showed antibiofilm activity, which makes them an interesting material to be used in different applications to counterattack global health problems due to the emergence of resistant microorganisms.Keywords: nickel, zinc, nanoparticles, antibiofilm activity, exopolysaccharide capping agent
format	article
author	Garza-Cervantes JA Escárcega-González CE Díaz Barriga Castro E Mendiola-Garza G Marichal-Cancino BA López-Vázquez MA Morones-Ramirez JR
author_facet	Garza-Cervantes JA Escárcega-González CE Díaz Barriga Castro E Mendiola-Garza G Marichal-Cancino BA López-Vázquez MA Morones-Ramirez JR
author_sort	Garza-Cervantes JA
title	Antimicrobial and antibiofilm activity of biopolymer-Ni, Zn nanoparticle biocomposites synthesized using R. mucilaginosa UANL-001L exopolysaccharide as a capping agent
title_short	Antimicrobial and antibiofilm activity of biopolymer-Ni, Zn nanoparticle biocomposites synthesized using R. mucilaginosa UANL-001L exopolysaccharide as a capping agent
title_full	Antimicrobial and antibiofilm activity of biopolymer-Ni, Zn nanoparticle biocomposites synthesized using R. mucilaginosa UANL-001L exopolysaccharide as a capping agent
title_fullStr	Antimicrobial and antibiofilm activity of biopolymer-Ni, Zn nanoparticle biocomposites synthesized using R. mucilaginosa UANL-001L exopolysaccharide as a capping agent
title_full_unstemmed	Antimicrobial and antibiofilm activity of biopolymer-Ni, Zn nanoparticle biocomposites synthesized using R. mucilaginosa UANL-001L exopolysaccharide as a capping agent
title_sort	antimicrobial and antibiofilm activity of biopolymer-ni, zn nanoparticle biocomposites synthesized using r. mucilaginosa uanl-001l exopolysaccharide as a capping agent
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/a4acfee33a0b49dfa835994f958624c4
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spelling	oai:doaj.org-article:a4acfee33a0b49dfa835994f958624c42021-12-02T04:33:56ZAntimicrobial and antibiofilm activity of biopolymer-Ni, Zn nanoparticle biocomposites synthesized using R. mucilaginosa UANL-001L exopolysaccharide as a capping agent1178-2013https://doaj.org/article/a4acfee33a0b49dfa835994f958624c42019-04-01T00:00:00Zhttps://www.dovepress.com/antimicrobial-and-antibiofilm-activity-of-biopolymer-ni-zn-nanoparticl-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Javier Alberto Garza-Cervantes,1,2 C Enrique Escárcega-González,1–3 E Díaz Barriga Castro,4 G Mendiola-Garza,1,2 Bruno Antonio Marichal-Cancino,3 Mario Alberto López-Vázquez,3 Jose Ruben Morones-Ramirez1,21Universidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Químicas, San Nicolás de los Garza, NL 66451, México; 2Centro de Investigación en Biotecnología y Nanotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, Parque de Investigación e Innovación Tecnológica, Apodaca 66629, Nuevo León, México; 3Universidad Autónoma de Aguascalientes, Departamento de Fisiología y Farmacología, Centro de Ciencias Básicas, Aguascalientes, Mexico; 4Centro de Investigación en Química Aplicada, Saltillo, Coahuila, MéxicoIntroduction: Global increase in the consumption of antibiotics has induced selective stress on wild-type microorganisms, pushing them to adapt to conditions of higher antibiotic concentrations, and thus an increased variety of resistant bacterial strains have emerged. Metal nanoparticles synthesized by green methods have been studied and proposed as potential antimicrobial agents against both wild-type and antibiotic-resistant strains; in addition, exopolysaccharides have been used as capping agent of metal nanoparticles due to their biocompatibility, reducing biological risks in a wide variety of applications.Purpose: In this work, we use an exopolysaccharide, from Rhodotorula mucilaginosa UANL-001L, an autochthonous strain from the Mexican northeast, as a capping agent in the synthesis of Zn, and Ni, nanoparticle biopolymer biocomposites.Materials and methods: To physically and chemically characterize the synthesized biocomposites, FT-IR, UV-Vs, TEM, SAED and EDS analysis were carried out. Antimicrobial and antibiofilm biological activity were tested for the biocomposites against two resistant clinical strains, a Gram-positive Staphylococcus aureus, and a Gram-negative Pseudomonas aeruginosa. Antimicrobial activity was determined using a microdilution assay whereas antibiofilm activity was analyzed through crystal violet staining.Results: Biocomposites composed of exopolysaccharide capped Zn and Ni metal nanoparticles were synthesized through a green synthesis methodology. The average size of the Zn and Ni nanoparticles ranged between 8 and 26 nm, respectively. The Ni-EPS biocomposites showed antimicrobial and antibiofilm activity against resistant strains of Staphylococcus aureus and Pseudomonas aeruginosa at 3 and 2 mg/mL, respectively. Moreover, Zn-EPS biocomposites showed antimicrobial activity against resistant Staphylococcus aureus at 1 mg/mL. Both biocomposites showed no toxicity, as renal function showed no differences between treatments and control in the in vivo assays with male rats tests in this study at a concentration of 24 mg/kg of body weight.Conclusion: The exopolysaccharide produced by Rhodotorula mucilaginosa UANL-001L is an excellent candidate as a capping agent in the synthesis of biopolymer-metal nanoparticle biocomposites. Both Ni and Zn-EPS biocomposites demonstrate to be potential contenders as novel antimicrobial agents against both Gram-negative and Gram-positive clinically relevant resistant bacterial strains. Moreover, Ni-EPS biocomposites also showed antibiofilm activity, which makes them an interesting material to be used in different applications to counterattack global health problems due to the emergence of resistant microorganisms.Keywords: nickel, zinc, nanoparticles, antibiofilm activity, exopolysaccharide capping agent  Garza-Cervantes JAEscárcega-González CEDíaz Barriga Castro EMendiola-Garza GMarichal-Cancino BALópez-Vázquez MAMorones-Ramirez JRDove Medical PressarticleNickelZincNanoparticlesAntibiofilm activityExopolysaccharide capping agentMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 2557-2571 (2019)

Antimicrobial and antibiofilm activity of biopolymer-Ni, Zn nanoparticle biocomposites synthesized using R. mucilaginosa UANL-001L exopolysaccharide as a capping agent

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