Biosynthesis of vitamins and cofactors in bacterium-harbouring trypanosomatids depends on the symbiotic association as revealed by genomic analyses.

Some non-pathogenic trypanosomatids maintain a mutualistic relationship with a betaproteobacterium of the Alcaligenaceae family. Intensive nutritional exchanges have been reported between the two partners, indicating that these protozoa are excellent biological models to study metabolic co-evolution...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cecilia C Klein, João M P Alves, Myrna G Serrano, Gregory A Buck, Ana Tereza R Vasconcelos, Marie-France Sagot, Marta M G Teixeira, Erney P Camargo, Maria Cristina M Motta
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a4b2a55d28af4a63a09cb714a0092338
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a4b2a55d28af4a63a09cb714a0092338
record_format	dspace
spelling	oai:doaj.org-article:a4b2a55d28af4a63a09cb714a00923382021-11-18T08:45:40ZBiosynthesis of vitamins and cofactors in bacterium-harbouring trypanosomatids depends on the symbiotic association as revealed by genomic analyses.1932-620310.1371/journal.pone.0079786https://doaj.org/article/a4b2a55d28af4a63a09cb714a00923382013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24260300/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Some non-pathogenic trypanosomatids maintain a mutualistic relationship with a betaproteobacterium of the Alcaligenaceae family. Intensive nutritional exchanges have been reported between the two partners, indicating that these protozoa are excellent biological models to study metabolic co-evolution. We previously sequenced and herein investigate the entire genomes of five trypanosomatids which harbor a symbiotic bacterium (SHTs for Symbiont-Haboring Trypanosomatids) and the respective bacteria (TPEs for Trypanosomatid Proteobacterial Endosymbiont), as well as two trypanosomatids without symbionts (RTs for Regular Trypanosomatids), for the presence of genes of the classical pathways for vitamin biosynthesis. Our data show that genes for the biosynthetic pathways of thiamine, biotin, and nicotinic acid are absent from all trypanosomatid genomes. This is in agreement with the absolute growth requirement for these vitamins in all protozoa of the family. Also absent from the genomes of RTs are the genes for the synthesis of pantothenic acid, folic acid, riboflavin, and vitamin B6. This is also in agreement with the available data showing that RTs are auxotrophic for these essential vitamins. On the other hand, SHTs are autotrophic for such vitamins. Indeed, all the genes of the corresponding biosynthetic pathways were identified, most of them in the symbiont genomes, while a few genes, mostly of eukaryotic origin, were found in the host genomes. The only exceptions to the latter are: the gene coding for the enzyme ketopantoate reductase (EC:1.1.1.169) which is related instead to the Firmicutes bacteria; and two other genes, one involved in the salvage pathway of pantothenic acid and the other in the synthesis of ubiquinone, that are related to Gammaproteobacteria. Their presence in trypanosomatids may result from lateral gene transfer. Taken together, our results reinforce the idea that the low nutritional requirement of SHTs is associated with the presence of the symbiotic bacterium, which contains most genes for vitamin production.Cecilia C KleinJoão M P AlvesMyrna G SerranoGregory A BuckAna Tereza R VasconcelosMarie-France SagotMarta M G TeixeiraErney P CamargoMaria Cristina M MottaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79786 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cecilia C Klein João M P Alves Myrna G Serrano Gregory A Buck Ana Tereza R Vasconcelos Marie-France Sagot Marta M G Teixeira Erney P Camargo Maria Cristina M Motta Biosynthesis of vitamins and cofactors in bacterium-harbouring trypanosomatids depends on the symbiotic association as revealed by genomic analyses.
description	Some non-pathogenic trypanosomatids maintain a mutualistic relationship with a betaproteobacterium of the Alcaligenaceae family. Intensive nutritional exchanges have been reported between the two partners, indicating that these protozoa are excellent biological models to study metabolic co-evolution. We previously sequenced and herein investigate the entire genomes of five trypanosomatids which harbor a symbiotic bacterium (SHTs for Symbiont-Haboring Trypanosomatids) and the respective bacteria (TPEs for Trypanosomatid Proteobacterial Endosymbiont), as well as two trypanosomatids without symbionts (RTs for Regular Trypanosomatids), for the presence of genes of the classical pathways for vitamin biosynthesis. Our data show that genes for the biosynthetic pathways of thiamine, biotin, and nicotinic acid are absent from all trypanosomatid genomes. This is in agreement with the absolute growth requirement for these vitamins in all protozoa of the family. Also absent from the genomes of RTs are the genes for the synthesis of pantothenic acid, folic acid, riboflavin, and vitamin B6. This is also in agreement with the available data showing that RTs are auxotrophic for these essential vitamins. On the other hand, SHTs are autotrophic for such vitamins. Indeed, all the genes of the corresponding biosynthetic pathways were identified, most of them in the symbiont genomes, while a few genes, mostly of eukaryotic origin, were found in the host genomes. The only exceptions to the latter are: the gene coding for the enzyme ketopantoate reductase (EC:1.1.1.169) which is related instead to the Firmicutes bacteria; and two other genes, one involved in the salvage pathway of pantothenic acid and the other in the synthesis of ubiquinone, that are related to Gammaproteobacteria. Their presence in trypanosomatids may result from lateral gene transfer. Taken together, our results reinforce the idea that the low nutritional requirement of SHTs is associated with the presence of the symbiotic bacterium, which contains most genes for vitamin production.
format	article
author	Cecilia C Klein João M P Alves Myrna G Serrano Gregory A Buck Ana Tereza R Vasconcelos Marie-France Sagot Marta M G Teixeira Erney P Camargo Maria Cristina M Motta
author_facet	Cecilia C Klein João M P Alves Myrna G Serrano Gregory A Buck Ana Tereza R Vasconcelos Marie-France Sagot Marta M G Teixeira Erney P Camargo Maria Cristina M Motta
author_sort	Cecilia C Klein
title	Biosynthesis of vitamins and cofactors in bacterium-harbouring trypanosomatids depends on the symbiotic association as revealed by genomic analyses.
title_short	Biosynthesis of vitamins and cofactors in bacterium-harbouring trypanosomatids depends on the symbiotic association as revealed by genomic analyses.
title_full	Biosynthesis of vitamins and cofactors in bacterium-harbouring trypanosomatids depends on the symbiotic association as revealed by genomic analyses.
title_fullStr	Biosynthesis of vitamins and cofactors in bacterium-harbouring trypanosomatids depends on the symbiotic association as revealed by genomic analyses.
title_full_unstemmed	Biosynthesis of vitamins and cofactors in bacterium-harbouring trypanosomatids depends on the symbiotic association as revealed by genomic analyses.
title_sort	biosynthesis of vitamins and cofactors in bacterium-harbouring trypanosomatids depends on the symbiotic association as revealed by genomic analyses.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a4b2a55d28af4a63a09cb714a0092338
work_keys_str_mv	AT ceciliacklein biosynthesisofvitaminsandcofactorsinbacteriumharbouringtrypanosomatidsdependsonthesymbioticassociationasrevealedbygenomicanalyses AT joaompalves biosynthesisofvitaminsandcofactorsinbacteriumharbouringtrypanosomatidsdependsonthesymbioticassociationasrevealedbygenomicanalyses AT myrnagserrano biosynthesisofvitaminsandcofactorsinbacteriumharbouringtrypanosomatidsdependsonthesymbioticassociationasrevealedbygenomicanalyses AT gregoryabuck biosynthesisofvitaminsandcofactorsinbacteriumharbouringtrypanosomatidsdependsonthesymbioticassociationasrevealedbygenomicanalyses AT anaterezarvasconcelos biosynthesisofvitaminsandcofactorsinbacteriumharbouringtrypanosomatidsdependsonthesymbioticassociationasrevealedbygenomicanalyses AT mariefrancesagot biosynthesisofvitaminsandcofactorsinbacteriumharbouringtrypanosomatidsdependsonthesymbioticassociationasrevealedbygenomicanalyses AT martamgteixeira biosynthesisofvitaminsandcofactorsinbacteriumharbouringtrypanosomatidsdependsonthesymbioticassociationasrevealedbygenomicanalyses AT erneypcamargo biosynthesisofvitaminsandcofactorsinbacteriumharbouringtrypanosomatidsdependsonthesymbioticassociationasrevealedbygenomicanalyses AT mariacristinammotta biosynthesisofvitaminsandcofactorsinbacteriumharbouringtrypanosomatidsdependsonthesymbioticassociationasrevealedbygenomicanalyses
_version_	1718421340323577856

Biosynthesis of vitamins and cofactors in bacterium-harbouring trypanosomatids depends on the symbiotic association as revealed by genomic analyses.

Ejemplares similares