Hematological, Micro-Rheological, and Metabolic Changes Modulated by Local Ischemic Pre- and Post-Conditioning in Rat Limb Ischemia-Reperfusion

In trauma and orthopedic surgery, limb ischemia-reperfusion (I/R) remains a great challenge. The effect of preventive protocols, including surgical conditioning approaches, is still controversial. We aimed to examine the effects of local ischemic pre-conditioning (PreC) and post-conditioning (PostC)...

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Autores principales:	Csaba Korei, Balazs Szabo, Adam Varga, Barbara Barath, Adam Deak, Erzsebet Vanyolos, Zoltan Hargitai, Ilona Kovacs, Norbert Nemeth, Katalin Peto
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	limb ischemia-reperfusion hemorheology metabolites ischemic pre-conditioning ischemic post-conditioning Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a4caa7fb70d9486c9aba3f827a9b2c7b
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spelling	oai:doaj.org-article:a4caa7fb70d9486c9aba3f827a9b2c7b2021-11-25T18:20:51ZHematological, Micro-Rheological, and Metabolic Changes Modulated by Local Ischemic Pre- and Post-Conditioning in Rat Limb Ischemia-Reperfusion10.3390/metabo111107762218-1989https://doaj.org/article/a4caa7fb70d9486c9aba3f827a9b2c7b2021-11-01T00:00:00Zhttps://www.mdpi.com/2218-1989/11/11/776https://doaj.org/toc/2218-1989In trauma and orthopedic surgery, limb ischemia-reperfusion (I/R) remains a great challenge. The effect of preventive protocols, including surgical conditioning approaches, is still controversial. We aimed to examine the effects of local ischemic pre-conditioning (PreC) and post-conditioning (PostC) on limb I/R. Anesthetized rats were randomized into sham-operated (control), I/R (120-min limb ischemia with tourniquet), PreC, or PostC groups (3 × 10-min tourniquet ischemia, 10-min reperfusion intervals). Blood samples were taken before and just after the ischemia, and on the first postoperative week for testing hematological, micro-rheological (erythrocyte deformability and aggregation), and metabolic parameters. Histological samples were also taken. Erythrocyte count, hemoglobin, and hematocrit values decreased, while after a temporary decrease, platelet count increased in I/R groups. Erythrocyte deformability impairment and aggregation enhancement were seen after ischemia, more obviously in the PreC group, and less in PostC. Blood pH decreased in all I/R groups. The elevation of creatinine and lactate concentration was the largest in PostC group. Histology did not reveal important differences. In conclusion, limb I/R caused micro-rheological impairment with hematological and metabolic changes. Ischemic pre- and post-conditioning had additive changes in various manners. Post-conditioning showed better micro-rheological effects. However, by these parameters it cannot be decided which protocol is better.Csaba KoreiBalazs SzaboAdam VargaBarbara BarathAdam DeakErzsebet VanyolosZoltan HargitaiIlona KovacsNorbert NemethKatalin PetoMDPI AGarticlelimb ischemia-reperfusionhemorheologymetabolitesischemic pre-conditioningischemic post-conditioningMicrobiologyQR1-502ENMetabolites, Vol 11, Iss 776, p 776 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	limb ischemia-reperfusion hemorheology metabolites ischemic pre-conditioning ischemic post-conditioning Microbiology QR1-502
spellingShingle	limb ischemia-reperfusion hemorheology metabolites ischemic pre-conditioning ischemic post-conditioning Microbiology QR1-502 Csaba Korei Balazs Szabo Adam Varga Barbara Barath Adam Deak Erzsebet Vanyolos Zoltan Hargitai Ilona Kovacs Norbert Nemeth Katalin Peto Hematological, Micro-Rheological, and Metabolic Changes Modulated by Local Ischemic Pre- and Post-Conditioning in Rat Limb Ischemia-Reperfusion
description	In trauma and orthopedic surgery, limb ischemia-reperfusion (I/R) remains a great challenge. The effect of preventive protocols, including surgical conditioning approaches, is still controversial. We aimed to examine the effects of local ischemic pre-conditioning (PreC) and post-conditioning (PostC) on limb I/R. Anesthetized rats were randomized into sham-operated (control), I/R (120-min limb ischemia with tourniquet), PreC, or PostC groups (3 × 10-min tourniquet ischemia, 10-min reperfusion intervals). Blood samples were taken before and just after the ischemia, and on the first postoperative week for testing hematological, micro-rheological (erythrocyte deformability and aggregation), and metabolic parameters. Histological samples were also taken. Erythrocyte count, hemoglobin, and hematocrit values decreased, while after a temporary decrease, platelet count increased in I/R groups. Erythrocyte deformability impairment and aggregation enhancement were seen after ischemia, more obviously in the PreC group, and less in PostC. Blood pH decreased in all I/R groups. The elevation of creatinine and lactate concentration was the largest in PostC group. Histology did not reveal important differences. In conclusion, limb I/R caused micro-rheological impairment with hematological and metabolic changes. Ischemic pre- and post-conditioning had additive changes in various manners. Post-conditioning showed better micro-rheological effects. However, by these parameters it cannot be decided which protocol is better.
format	article
author	Csaba Korei Balazs Szabo Adam Varga Barbara Barath Adam Deak Erzsebet Vanyolos Zoltan Hargitai Ilona Kovacs Norbert Nemeth Katalin Peto
author_facet	Csaba Korei Balazs Szabo Adam Varga Barbara Barath Adam Deak Erzsebet Vanyolos Zoltan Hargitai Ilona Kovacs Norbert Nemeth Katalin Peto
author_sort	Csaba Korei
title	Hematological, Micro-Rheological, and Metabolic Changes Modulated by Local Ischemic Pre- and Post-Conditioning in Rat Limb Ischemia-Reperfusion
title_short	Hematological, Micro-Rheological, and Metabolic Changes Modulated by Local Ischemic Pre- and Post-Conditioning in Rat Limb Ischemia-Reperfusion
title_full	Hematological, Micro-Rheological, and Metabolic Changes Modulated by Local Ischemic Pre- and Post-Conditioning in Rat Limb Ischemia-Reperfusion
title_fullStr	Hematological, Micro-Rheological, and Metabolic Changes Modulated by Local Ischemic Pre- and Post-Conditioning in Rat Limb Ischemia-Reperfusion
title_full_unstemmed	Hematological, Micro-Rheological, and Metabolic Changes Modulated by Local Ischemic Pre- and Post-Conditioning in Rat Limb Ischemia-Reperfusion
title_sort	hematological, micro-rheological, and metabolic changes modulated by local ischemic pre- and post-conditioning in rat limb ischemia-reperfusion
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a4caa7fb70d9486c9aba3f827a9b2c7b
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Hematological, Micro-Rheological, and Metabolic Changes Modulated by Local Ischemic Pre- and Post-Conditioning in Rat Limb Ischemia-Reperfusion

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