Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers

Abstract Growth factor-eluting polymer systems have been widely reported to improve cell and tissue outcomes; however, measurements of actual growth factor concentration in cell culture conditions are limited. The problem is compounded by a lack of knowledge of growth factor half-lives, which impede...

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Autores principales:	Ivan Ding, Amy M. Peterson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a4d30666bc5a4ff6be41e1846f35baad
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spelling	oai:doaj.org-article:a4d30666bc5a4ff6be41e1846f35baad2021-12-02T14:29:09ZHalf-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers10.1038/s41598-021-89229-w2045-2322https://doaj.org/article/a4d30666bc5a4ff6be41e1846f35baad2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89229-whttps://doaj.org/toc/2045-2322Abstract Growth factor-eluting polymer systems have been widely reported to improve cell and tissue outcomes; however, measurements of actual growth factor concentration in cell culture conditions are limited. The problem is compounded by a lack of knowledge of growth factor half-lives, which impedes efforts to determine real-time growth factor concentrations. In this work, the half-life of basic fibroblast growth factor (FGF2) was determined using enzyme linked immunosorbent assay (ELISA). FGF2 release from polyelectrolyte multilayers (PEMs) was measured and the data was fit to a simple degradation model, allowing for the determination of FGF2 concentrations between 2 and 4 days of culture time. After the first hour, the FGF2 concentration for PEMs assembled at pH = 4 ranged from 2.67 ng/mL to 5.76 ng/mL, while for PEMs assembled at pH = 5, the concentration ranged from 0.62 ng/mL to 2.12 ng/mL. CRL-2352 fibroblasts were cultured on PEMs assembled at pH = 4 and pH = 5. After 2 days, the FGF2-eluting PEM conditions showed improved cell count and spreading. After 4 days, only the pH = 4 assembly condition had higher cells counts, while the PEM assembled at pH = 5 and PEM with no FGF2 showed increased spreading. Overall, the half-life model and cell culture study provide optimal concentration ranges for fibroblast proliferation and a framework for understanding how temporal FGF2 concentration may affect other cell types.Ivan DingAmy M. PetersonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ivan Ding Amy M. Peterson Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers
description	Abstract Growth factor-eluting polymer systems have been widely reported to improve cell and tissue outcomes; however, measurements of actual growth factor concentration in cell culture conditions are limited. The problem is compounded by a lack of knowledge of growth factor half-lives, which impedes efforts to determine real-time growth factor concentrations. In this work, the half-life of basic fibroblast growth factor (FGF2) was determined using enzyme linked immunosorbent assay (ELISA). FGF2 release from polyelectrolyte multilayers (PEMs) was measured and the data was fit to a simple degradation model, allowing for the determination of FGF2 concentrations between 2 and 4 days of culture time. After the first hour, the FGF2 concentration for PEMs assembled at pH = 4 ranged from 2.67 ng/mL to 5.76 ng/mL, while for PEMs assembled at pH = 5, the concentration ranged from 0.62 ng/mL to 2.12 ng/mL. CRL-2352 fibroblasts were cultured on PEMs assembled at pH = 4 and pH = 5. After 2 days, the FGF2-eluting PEM conditions showed improved cell count and spreading. After 4 days, only the pH = 4 assembly condition had higher cells counts, while the PEM assembled at pH = 5 and PEM with no FGF2 showed increased spreading. Overall, the half-life model and cell culture study provide optimal concentration ranges for fibroblast proliferation and a framework for understanding how temporal FGF2 concentration may affect other cell types.
format	article
author	Ivan Ding Amy M. Peterson
author_facet	Ivan Ding Amy M. Peterson
author_sort	Ivan Ding
title	Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers
title_short	Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers
title_full	Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers
title_fullStr	Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers
title_full_unstemmed	Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers
title_sort	half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a4d30666bc5a4ff6be41e1846f35baad
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Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers

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