Serum cytokine profiles in healthy nonhuman primates are blunted by sedation and demonstrate sexual dimorphism as detected by a validated multiplex immunoassay

Abstract Cytokine profiling is a valuable tool for monitoring immune responses associated with disease and treatment. This study assessed the impact of sex and sedation on serum cytokines in healthy nonhuman primates (NHPs). Twenty-three cytokines were measured from serum using a bead-based multiple...

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Autores principales:	Laura Hocum Stone, Scott Hunter Oppler, Julia L. Nugent, Sarah Gresch, Bernhard J. Hering, Michael P. Murtaugh, Rebecca L. Hegstad-Davies, Sabarinathan Ramachandran, Melanie L. Graham
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a4e0c2066c624879b3e74792408b6da4
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spelling	oai:doaj.org-article:a4e0c2066c624879b3e74792408b6da42021-12-02T13:24:07ZSerum cytokine profiles in healthy nonhuman primates are blunted by sedation and demonstrate sexual dimorphism as detected by a validated multiplex immunoassay10.1038/s41598-021-81953-72045-2322https://doaj.org/article/a4e0c2066c624879b3e74792408b6da42021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81953-7https://doaj.org/toc/2045-2322Abstract Cytokine profiling is a valuable tool for monitoring immune responses associated with disease and treatment. This study assessed the impact of sex and sedation on serum cytokines in healthy nonhuman primates (NHPs). Twenty-three cytokines were measured from serum using a bead-based multiplex assay. Assay validation for precision, sensitivity, recovery, linearity, and stability was performed. Samples from male and female cynomolgus and rhesus macaques either cooperating or sedated were compared. All cytokines except TNFα demonstrated acceptable sensitivity and precision, with variable recovery and linearity. IFNγ, IL-2, IL-5, IL-6, IL-8, IL-12/23 (p40), IL-13, IL-15, MCP-1, TGFα, VEGF met acceptance criteria; G-CSF, IL-4, IL-10, MIP1α, sCD40L were marginal. Higher cytokine levels were observed in females and cytokine levels were blunted in sedated NHPs when compared to awake cooperating NHPs. Significant differences observed in cytokines related to sex, species, or imposed by handling highlight the importance of model design on translational relevance for clinical settings.Laura Hocum StoneScott Hunter OpplerJulia L. NugentSarah GreschBernhard J. HeringMichael P. MurtaughRebecca L. Hegstad-DaviesSabarinathan RamachandranMelanie L. GrahamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laura Hocum Stone Scott Hunter Oppler Julia L. Nugent Sarah Gresch Bernhard J. Hering Michael P. Murtaugh Rebecca L. Hegstad-Davies Sabarinathan Ramachandran Melanie L. Graham Serum cytokine profiles in healthy nonhuman primates are blunted by sedation and demonstrate sexual dimorphism as detected by a validated multiplex immunoassay
description	Abstract Cytokine profiling is a valuable tool for monitoring immune responses associated with disease and treatment. This study assessed the impact of sex and sedation on serum cytokines in healthy nonhuman primates (NHPs). Twenty-three cytokines were measured from serum using a bead-based multiplex assay. Assay validation for precision, sensitivity, recovery, linearity, and stability was performed. Samples from male and female cynomolgus and rhesus macaques either cooperating or sedated were compared. All cytokines except TNFα demonstrated acceptable sensitivity and precision, with variable recovery and linearity. IFNγ, IL-2, IL-5, IL-6, IL-8, IL-12/23 (p40), IL-13, IL-15, MCP-1, TGFα, VEGF met acceptance criteria; G-CSF, IL-4, IL-10, MIP1α, sCD40L were marginal. Higher cytokine levels were observed in females and cytokine levels were blunted in sedated NHPs when compared to awake cooperating NHPs. Significant differences observed in cytokines related to sex, species, or imposed by handling highlight the importance of model design on translational relevance for clinical settings.
format	article
author	Laura Hocum Stone Scott Hunter Oppler Julia L. Nugent Sarah Gresch Bernhard J. Hering Michael P. Murtaugh Rebecca L. Hegstad-Davies Sabarinathan Ramachandran Melanie L. Graham
author_facet	Laura Hocum Stone Scott Hunter Oppler Julia L. Nugent Sarah Gresch Bernhard J. Hering Michael P. Murtaugh Rebecca L. Hegstad-Davies Sabarinathan Ramachandran Melanie L. Graham
author_sort	Laura Hocum Stone
title	Serum cytokine profiles in healthy nonhuman primates are blunted by sedation and demonstrate sexual dimorphism as detected by a validated multiplex immunoassay
title_short	Serum cytokine profiles in healthy nonhuman primates are blunted by sedation and demonstrate sexual dimorphism as detected by a validated multiplex immunoassay
title_full	Serum cytokine profiles in healthy nonhuman primates are blunted by sedation and demonstrate sexual dimorphism as detected by a validated multiplex immunoassay
title_fullStr	Serum cytokine profiles in healthy nonhuman primates are blunted by sedation and demonstrate sexual dimorphism as detected by a validated multiplex immunoassay
title_full_unstemmed	Serum cytokine profiles in healthy nonhuman primates are blunted by sedation and demonstrate sexual dimorphism as detected by a validated multiplex immunoassay
title_sort	serum cytokine profiles in healthy nonhuman primates are blunted by sedation and demonstrate sexual dimorphism as detected by a validated multiplex immunoassay
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a4e0c2066c624879b3e74792408b6da4
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Serum cytokine profiles in healthy nonhuman primates are blunted by sedation and demonstrate sexual dimorphism as detected by a validated multiplex immunoassay

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