Preparation and purification of anti immunoglobulin Y antibodies from rabbit

Background and Objective: Chicken egg yolk antibodies (IgY) play an important role as an alternative to mammalian polyclonal antibodies. They are recently used in medical research for prophylaxis, diagnosis and treatment of diseases. Anti-IgY antibodies are used for immunoassay and therapy. The aim...

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Autores principales:	M Pour Amir, S Mahjoub, GH Maliji
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2004
Materias:	immunoglobulin y anti-igy antibody rabbit purification Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a4e13d9d629c42e4b4f8039af1c0e708
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spelling	oai:doaj.org-article:a4e13d9d629c42e4b4f8039af1c0e7082021-11-10T09:15:56ZPreparation and purification of anti immunoglobulin Y antibodies from rabbit1561-41072251-7170https://doaj.org/article/a4e13d9d629c42e4b4f8039af1c0e7082004-01-01T00:00:00Zhttp://jbums.org/article-1-2732-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170Background and Objective: Chicken egg yolk antibodies (IgY) play an important role as an alternative to mammalian polyclonal antibodies. They are recently used in medical research for prophylaxis, diagnosis and treatment of diseases. Anti-IgY antibodies are used for immunoassay and therapy. The aim of this study was preparation and purification of anti-immunoglobulin Y antibodies from rabbit serum and confirm of its activity. Methods: IgY and other water-soluble proteins were extracted with acid and chloroform and then thiophilic chromatography (T-gel) was performed and applied for purification of IgY. Two female rabbits were immunized with IgY in Freud’s adjuvant and then booster doses were administered, blood samples were collected by times. Ammonium sulfate precipitation, then gel filtration or T-gel chromatography used for purification of Anti-IgY antibodies. Cellulous acetate electrophoresis, double immunodiffusion and ELISA procedures used for confirm of identification and activity. Findings: The purified protein (IgY) was observed in the γ-globulin region of electrophoresis. The purified antibodies from rabbit serum were observed in the γ-globulin region, which reacted against IgY in double immunodiffusion and the titer of purified antibody-using ELISA, 1.10000 obtained. Conclusion: These antibodies serve as the basis of many immunoassays for detection and assay of several molecules as anti-immunoglobulin Y reagents.M Pour AmirS MahjoubGH MalijiBabol University of Medical Sciencesarticleimmunoglobulin yanti-igy antibodyrabbitpurificationMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 6, Iss 1, Pp 7-13 (2004)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	immunoglobulin y anti-igy antibody rabbit purification Medicine R Medicine (General) R5-920
spellingShingle	immunoglobulin y anti-igy antibody rabbit purification Medicine R Medicine (General) R5-920 M Pour Amir S Mahjoub GH Maliji Preparation and purification of anti immunoglobulin Y antibodies from rabbit
description	Background and Objective: Chicken egg yolk antibodies (IgY) play an important role as an alternative to mammalian polyclonal antibodies. They are recently used in medical research for prophylaxis, diagnosis and treatment of diseases. Anti-IgY antibodies are used for immunoassay and therapy. The aim of this study was preparation and purification of anti-immunoglobulin Y antibodies from rabbit serum and confirm of its activity. Methods: IgY and other water-soluble proteins were extracted with acid and chloroform and then thiophilic chromatography (T-gel) was performed and applied for purification of IgY. Two female rabbits were immunized with IgY in Freud’s adjuvant and then booster doses were administered, blood samples were collected by times. Ammonium sulfate precipitation, then gel filtration or T-gel chromatography used for purification of Anti-IgY antibodies. Cellulous acetate electrophoresis, double immunodiffusion and ELISA procedures used for confirm of identification and activity. Findings: The purified protein (IgY) was observed in the γ-globulin region of electrophoresis. The purified antibodies from rabbit serum were observed in the γ-globulin region, which reacted against IgY in double immunodiffusion and the titer of purified antibody-using ELISA, 1.10000 obtained. Conclusion: These antibodies serve as the basis of many immunoassays for detection and assay of several molecules as anti-immunoglobulin Y reagents.
format	article
author	M Pour Amir S Mahjoub GH Maliji
author_facet	M Pour Amir S Mahjoub GH Maliji
author_sort	M Pour Amir
title	Preparation and purification of anti immunoglobulin Y antibodies from rabbit
title_short	Preparation and purification of anti immunoglobulin Y antibodies from rabbit
title_full	Preparation and purification of anti immunoglobulin Y antibodies from rabbit
title_fullStr	Preparation and purification of anti immunoglobulin Y antibodies from rabbit
title_full_unstemmed	Preparation and purification of anti immunoglobulin Y antibodies from rabbit
title_sort	preparation and purification of anti immunoglobulin y antibodies from rabbit
publisher	Babol University of Medical Sciences
publishDate	2004
url	https://doaj.org/article/a4e13d9d629c42e4b4f8039af1c0e708
work_keys_str_mv	AT mpouramir preparationandpurificationofantiimmunoglobulinyantibodiesfromrabbit AT smahjoub preparationandpurificationofantiimmunoglobulinyantibodiesfromrabbit AT ghmaliji preparationandpurificationofantiimmunoglobulinyantibodiesfromrabbit
_version_	1718440222512906240

Preparation and purification of anti immunoglobulin Y antibodies from rabbit

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