Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents

Sougata Ghosh1, Sumersing Patil1, Mehul Ahire1, Rohini Kitture2, Sangeeta Kale3, Karishma Pardesi4, Swaranjit S Cameotra5, Jayesh Bellare6, Dilip D Dhavale7, Amit Jabgunde7, Balu A Chopade11Institute of Bioinformatics and Biotechnology, University of Pune, Pune, 2Department of Electronic Science, Fe...

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Autores principales:	Ghosh S, Patil S, Ahire M, Kitture R, Kale S, Pardesi K, Cameotra SS, Bellare J, Dhavale DD, Jabgunde A, Chopade BA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a4e2ec8d6a024b07b2a912400078423d
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spelling	oai:doaj.org-article:a4e2ec8d6a024b07b2a912400078423d2021-12-02T05:02:07ZSynthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents1176-91141178-2013https://doaj.org/article/a4e2ec8d6a024b07b2a912400078423d2012-02-01T00:00:00Zhttp://www.dovepress.com/synthesis-of-silver-nanoparticles-using-dioscorea-bulbifera-tuber-extr-a9183https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Sougata Ghosh1, Sumersing Patil1, Mehul Ahire1, Rohini Kitture2, Sangeeta Kale3, Karishma Pardesi4, Swaranjit S Cameotra5, Jayesh Bellare6, Dilip D Dhavale7, Amit Jabgunde7, Balu A Chopade11Institute of Bioinformatics and Biotechnology, University of Pune, Pune, 2Department of Electronic Science, Fergusson College, Pune, 3Department of Applied Physics, Defense Institute of Advanced Technology, Girinagar, Pune, 4Department of Microbiology, University of Pune, Pune, 5Institute of Microbial Technology, Chandigarh, 6Department of Chemical Engineering, Indian Institute of Technology, Mumbai, 7Garware Research Centre, Department of Chemistry, University of Pune, Pune, IndiaBackground: Development of an environmentally benign process for the synthesis of silver nanomaterials is an important aspect of current nanotechnology research. Among the 600 species of the genus Dioscorea, Dioscorea bulbifera has profound therapeutic applications due to its unique phytochemistry. In this paper, we report on the rapid synthesis of silver nanoparticles by reduction of aqueous Ag+ ions using D. bulbifera tuber extract.Methods and results: Phytochemical analysis revealed that D. bulbifera tuber extract is rich in flavonoid, phenolics, reducing sugars, starch, diosgenin, ascorbic acid, and citric acid. The biosynthesis process was quite fast, and silver nanoparticles were formed within 5 hours. Ultraviolet-visible absorption spectroscopy, transmission electron microscopy, high-resolution transmission electron microscopy, energy dispersive spectroscopy, and x-ray diffraction confirmed reduction of the Ag+ ions. Varied morphology of the bioreduced silver nanoparticles included spheres, triangles, and hexagons. Optimization studies revealed that the maximum rate of synthesis could be achieved with 0.7 mM AgNO3 solution at 50&deg;C in 5 hours. The resulting silver nanoparticles were found to possess potent antibacterial activity against both Gram-negative and Gram-positive bacteria. Beta-lactam (piperacillin) and macrolide (erythromycin) antibiotics showed a 3.6-fold and 3-fold increase, respectively, in combination with silver nanoparticles selectively against multidrug-resistant Acinetobacter baumannii. Notable synergy was seen between silver nanoparticles and chloramphenicol or vancomycin against Pseudomonas aeruginosa, and was supported by a 4.9-fold and 4.2-fold increase in zone diameter, respectively. Similarly, we found a maximum 11.8-fold increase in zone diameter of streptomycin when combined with silver nanoparticles against E. coli, providing strong evidence for the synergistic action of a combination of antibiotics and silver nanoparticles.Conclusion: This is the first report on the synthesis of silver nanoparticles using D. bulbifera tuber extract followed by an estimation of its synergistic potential for enhancement of the antibacterial activity of broad spectrum antimicrobial agents.Keywords: Dioscorea bulbifera tuber extract, silver nanoparticles, antimicrobial synergyGhosh SPatil SAhire MKitture RKale SPardesi KCameotra SSBellare JDhavale DDJabgunde AChopade BADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 483-496 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ghosh S Patil S Ahire M Kitture R Kale S Pardesi K Cameotra SS Bellare J Dhavale DD Jabgunde A Chopade BA Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents
description	Sougata Ghosh1, Sumersing Patil1, Mehul Ahire1, Rohini Kitture2, Sangeeta Kale3, Karishma Pardesi4, Swaranjit S Cameotra5, Jayesh Bellare6, Dilip D Dhavale7, Amit Jabgunde7, Balu A Chopade11Institute of Bioinformatics and Biotechnology, University of Pune, Pune, 2Department of Electronic Science, Fergusson College, Pune, 3Department of Applied Physics, Defense Institute of Advanced Technology, Girinagar, Pune, 4Department of Microbiology, University of Pune, Pune, 5Institute of Microbial Technology, Chandigarh, 6Department of Chemical Engineering, Indian Institute of Technology, Mumbai, 7Garware Research Centre, Department of Chemistry, University of Pune, Pune, IndiaBackground: Development of an environmentally benign process for the synthesis of silver nanomaterials is an important aspect of current nanotechnology research. Among the 600 species of the genus Dioscorea, Dioscorea bulbifera has profound therapeutic applications due to its unique phytochemistry. In this paper, we report on the rapid synthesis of silver nanoparticles by reduction of aqueous Ag+ ions using D. bulbifera tuber extract.Methods and results: Phytochemical analysis revealed that D. bulbifera tuber extract is rich in flavonoid, phenolics, reducing sugars, starch, diosgenin, ascorbic acid, and citric acid. The biosynthesis process was quite fast, and silver nanoparticles were formed within 5 hours. Ultraviolet-visible absorption spectroscopy, transmission electron microscopy, high-resolution transmission electron microscopy, energy dispersive spectroscopy, and x-ray diffraction confirmed reduction of the Ag+ ions. Varied morphology of the bioreduced silver nanoparticles included spheres, triangles, and hexagons. Optimization studies revealed that the maximum rate of synthesis could be achieved with 0.7 mM AgNO3 solution at 50&deg;C in 5 hours. The resulting silver nanoparticles were found to possess potent antibacterial activity against both Gram-negative and Gram-positive bacteria. Beta-lactam (piperacillin) and macrolide (erythromycin) antibiotics showed a 3.6-fold and 3-fold increase, respectively, in combination with silver nanoparticles selectively against multidrug-resistant Acinetobacter baumannii. Notable synergy was seen between silver nanoparticles and chloramphenicol or vancomycin against Pseudomonas aeruginosa, and was supported by a 4.9-fold and 4.2-fold increase in zone diameter, respectively. Similarly, we found a maximum 11.8-fold increase in zone diameter of streptomycin when combined with silver nanoparticles against E. coli, providing strong evidence for the synergistic action of a combination of antibiotics and silver nanoparticles.Conclusion: This is the first report on the synthesis of silver nanoparticles using D. bulbifera tuber extract followed by an estimation of its synergistic potential for enhancement of the antibacterial activity of broad spectrum antimicrobial agents.Keywords: Dioscorea bulbifera tuber extract, silver nanoparticles, antimicrobial synergy
format	article
author	Ghosh S Patil S Ahire M Kitture R Kale S Pardesi K Cameotra SS Bellare J Dhavale DD Jabgunde A Chopade BA
author_facet	Ghosh S Patil S Ahire M Kitture R Kale S Pardesi K Cameotra SS Bellare J Dhavale DD Jabgunde A Chopade BA
author_sort	Ghosh S
title	Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents
title_short	Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents
title_full	Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents
title_fullStr	Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents
title_full_unstemmed	Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents
title_sort	synthesis of silver nanoparticles using dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/a4e2ec8d6a024b07b2a912400078423d
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Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents

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