Campylobacter jejuni in Different Canine Populations: Characteristics and Zoonotic Potential

With most epidemiological studies focused on poultry, dogs are often overlooked as a reservoir of Campylobacter, even though these animals maintain close daily contact with humans. The present study aimed to obtain a first insight into the presence and characteristics of ...

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Autores principales:	Maria-Leonor Lemos, Alexandra Nunes, Massimo Ancora, Cesare Cammà, Paulo Martins da Costa, Mónica Oleastro
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Campylobacter</i> spp. dogs whole genome sequencing One Health Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a4e45df4bc2246e0a971bf75b4ac11d5
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id	oai:doaj.org-article:a4e45df4bc2246e0a971bf75b4ac11d5
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spelling	oai:doaj.org-article:a4e45df4bc2246e0a971bf75b4ac11d52021-11-25T18:24:24Z<i>Campylobacter jejuni</i> in Different Canine Populations: Characteristics and Zoonotic Potential10.3390/microorganisms91122312076-2607https://doaj.org/article/a4e45df4bc2246e0a971bf75b4ac11d52021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2231https://doaj.org/toc/2076-2607With most epidemiological studies focused on poultry, dogs are often overlooked as a reservoir of <i>Campylobacter</i>, even though these animals maintain close daily contact with humans. The present study aimed to obtain a first insight into the presence and characteristics of <i>Campylobacter</i> spp. in different canine populations in Portugal, and to evaluate its zoonotic potential through genomic analysis. From a total of 125 rectal swabs collected from companion (<i>n</i> = 71) and hunting dogs (<i>n</i> = 54) living in two different settings, rural (<i>n</i> = 75) and urban (<i>n</i> = 50), 32 <i>Campylobacter</i> spp. isolates were obtained. Four different <i>Campylobacter</i> species were identified by Multiplex PCR and MALDI-TOF mass spectrometry, of which <i>Campylobacter jejuni</i> (<i>n</i> = 14, 44%) was overall the most frequently found species. Relevant resistance phenotypes were detected in <i>C. jejuni,</i> with 93% of the isolates being resistant to ciprofloxacin, 64% to tetracycline, and 57% to ampicillin, and three isolates being multi-drug-resistant. Comparison of the phenotypic and genotypic traits with human isolates from Portuguese patients revealed great similarity between both groups. Particularly relevant, the wgMLST analysis allowed the identification of isolates from human and dogs without any apparent epidemiological relationship, sharing high genetic proximity. Notwithstanding the limited sample size, considering the high genomic diversity of <i>C. jejuni,</i> the genetic overlap between human and dog strains observed in this study confirmed that the occurrence of this species in dogs is of public health concern, reinforcing the call for a One Health approach.Maria-Leonor LemosAlexandra NunesMassimo AncoraCesare CammàPaulo Martins da CostaMónica OleastroMDPI AGarticle<i>Campylobacter</i> spp.dogswhole genome sequencingOne HealthBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2231, p 2231 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Campylobacter</i> spp. dogs whole genome sequencing One Health Biology (General) QH301-705.5
spellingShingle	<i>Campylobacter</i> spp. dogs whole genome sequencing One Health Biology (General) QH301-705.5 Maria-Leonor Lemos Alexandra Nunes Massimo Ancora Cesare Cammà Paulo Martins da Costa Mónica Oleastro <i>Campylobacter jejuni</i> in Different Canine Populations: Characteristics and Zoonotic Potential
description	With most epidemiological studies focused on poultry, dogs are often overlooked as a reservoir of <i>Campylobacter</i>, even though these animals maintain close daily contact with humans. The present study aimed to obtain a first insight into the presence and characteristics of <i>Campylobacter</i> spp. in different canine populations in Portugal, and to evaluate its zoonotic potential through genomic analysis. From a total of 125 rectal swabs collected from companion (<i>n</i> = 71) and hunting dogs (<i>n</i> = 54) living in two different settings, rural (<i>n</i> = 75) and urban (<i>n</i> = 50), 32 <i>Campylobacter</i> spp. isolates were obtained. Four different <i>Campylobacter</i> species were identified by Multiplex PCR and MALDI-TOF mass spectrometry, of which <i>Campylobacter jejuni</i> (<i>n</i> = 14, 44%) was overall the most frequently found species. Relevant resistance phenotypes were detected in <i>C. jejuni,</i> with 93% of the isolates being resistant to ciprofloxacin, 64% to tetracycline, and 57% to ampicillin, and three isolates being multi-drug-resistant. Comparison of the phenotypic and genotypic traits with human isolates from Portuguese patients revealed great similarity between both groups. Particularly relevant, the wgMLST analysis allowed the identification of isolates from human and dogs without any apparent epidemiological relationship, sharing high genetic proximity. Notwithstanding the limited sample size, considering the high genomic diversity of <i>C. jejuni,</i> the genetic overlap between human and dog strains observed in this study confirmed that the occurrence of this species in dogs is of public health concern, reinforcing the call for a One Health approach.
format	article
author	Maria-Leonor Lemos Alexandra Nunes Massimo Ancora Cesare Cammà Paulo Martins da Costa Mónica Oleastro
author_facet	Maria-Leonor Lemos Alexandra Nunes Massimo Ancora Cesare Cammà Paulo Martins da Costa Mónica Oleastro
author_sort	Maria-Leonor Lemos
title	<i>Campylobacter jejuni</i> in Different Canine Populations: Characteristics and Zoonotic Potential
title_short	<i>Campylobacter jejuni</i> in Different Canine Populations: Characteristics and Zoonotic Potential
title_full	<i>Campylobacter jejuni</i> in Different Canine Populations: Characteristics and Zoonotic Potential
title_fullStr	<i>Campylobacter jejuni</i> in Different Canine Populations: Characteristics and Zoonotic Potential
title_full_unstemmed	<i>Campylobacter jejuni</i> in Different Canine Populations: Characteristics and Zoonotic Potential
title_sort	<i>campylobacter jejuni</i> in different canine populations: characteristics and zoonotic potential
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a4e45df4bc2246e0a971bf75b4ac11d5
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<i>Campylobacter jejuni</i> in Different Canine Populations: Characteristics and Zoonotic Potential

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