Relationship between age/gender-induced survival changes and the magnitude of inflammatory activation and organ dysfunction in post-traumatic sepsis.
Age/gender may likely influence the course of septic complications after trauma. We aimed to characterize the influence of age/gender on the response of circulating cytokines, cells and organ function in post-traumatic sepsis. We additionally tested whether post-traumatic responses alone can accurat...
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2012
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oai:doaj.org-article:a4e7a65599224057bdaa71109c76b11f2021-11-18T08:05:20ZRelationship between age/gender-induced survival changes and the magnitude of inflammatory activation and organ dysfunction in post-traumatic sepsis.1932-620310.1371/journal.pone.0051457https://doaj.org/article/a4e7a65599224057bdaa71109c76b11f2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23251540/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Age/gender may likely influence the course of septic complications after trauma. We aimed to characterize the influence of age/gender on the response of circulating cytokines, cells and organ function in post-traumatic sepsis. We additionally tested whether post-traumatic responses alone can accurately predict outcomes in subsequent post-traumatic sepsis. A mouse 2-hit model of trauma/hemorrhage (TH, 1(st) hit) and cecal ligation and puncture (CLP, 2(nd) hit) was employed. 3, 15 and 20 month (m) old female (♀) and male (♂) CD-1 mice underwent sublethal TH followed by CLP 2 days later. Blood was sampled daily until day 6 post-TH and survival was followed for 16 days. To compare general response patterns among groups, we calculated two scores: the inflammatory response (including KC, MIP-1α, TNFα, MCP-1, IFNγ, IL-1β,-5,-6,-10) and the organ dysfunction score (Urea, ALT, AST and LDH). Moreover, mice were retrospectively divided into survivors (SUR) and dying (DIE) based on post-CLP outcome. In general, females survived better than males and their survival did not correspond to any specific estrus cycle phase. Pre-CLP phase: the post-TH inflammatory score was weakest in 3 m♂ but there were no changes among remaining groups (similar lack of differences in the organ dysfunction score). TH induced a 40% increase of IFNγ, MIP-1α and IL-5 in 15 m♂ SUR (vs. DIE) but predictive accuracy for post-CLP outcomes was moderate. Post-CLP phase: while stable in males, inflammatory response score in 15 m and 20 m females decreased with age at day 1 and 2 post-CLP. SUR vs. DIE differences in inflammatory and organ dysfunction score were evident but their magnitude was comparable across age/gender. Nearly identical activation of the humoral inflammatory and organ function compartments, both across groups and according to sepsis severity, suggests that they are not directly responsible for the age/gender-dependent disparity in TH-CLP survival in the studied young-to-mature population.Susanne DrechslerKatrin WeixelbaumerPierre RaevenMohammad JafarmadarAnna KhademMartijn van GriensvenSoheyl BahramiMarcin Filip OsuchowskiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e51457 (2012) |
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Medicine R Science Q Susanne Drechsler Katrin Weixelbaumer Pierre Raeven Mohammad Jafarmadar Anna Khadem Martijn van Griensven Soheyl Bahrami Marcin Filip Osuchowski Relationship between age/gender-induced survival changes and the magnitude of inflammatory activation and organ dysfunction in post-traumatic sepsis. |
| description |
Age/gender may likely influence the course of septic complications after trauma. We aimed to characterize the influence of age/gender on the response of circulating cytokines, cells and organ function in post-traumatic sepsis. We additionally tested whether post-traumatic responses alone can accurately predict outcomes in subsequent post-traumatic sepsis. A mouse 2-hit model of trauma/hemorrhage (TH, 1(st) hit) and cecal ligation and puncture (CLP, 2(nd) hit) was employed. 3, 15 and 20 month (m) old female (♀) and male (♂) CD-1 mice underwent sublethal TH followed by CLP 2 days later. Blood was sampled daily until day 6 post-TH and survival was followed for 16 days. To compare general response patterns among groups, we calculated two scores: the inflammatory response (including KC, MIP-1α, TNFα, MCP-1, IFNγ, IL-1β,-5,-6,-10) and the organ dysfunction score (Urea, ALT, AST and LDH). Moreover, mice were retrospectively divided into survivors (SUR) and dying (DIE) based on post-CLP outcome. In general, females survived better than males and their survival did not correspond to any specific estrus cycle phase. Pre-CLP phase: the post-TH inflammatory score was weakest in 3 m♂ but there were no changes among remaining groups (similar lack of differences in the organ dysfunction score). TH induced a 40% increase of IFNγ, MIP-1α and IL-5 in 15 m♂ SUR (vs. DIE) but predictive accuracy for post-CLP outcomes was moderate. Post-CLP phase: while stable in males, inflammatory response score in 15 m and 20 m females decreased with age at day 1 and 2 post-CLP. SUR vs. DIE differences in inflammatory and organ dysfunction score were evident but their magnitude was comparable across age/gender. Nearly identical activation of the humoral inflammatory and organ function compartments, both across groups and according to sepsis severity, suggests that they are not directly responsible for the age/gender-dependent disparity in TH-CLP survival in the studied young-to-mature population. |
| format |
article |
| author |
Susanne Drechsler Katrin Weixelbaumer Pierre Raeven Mohammad Jafarmadar Anna Khadem Martijn van Griensven Soheyl Bahrami Marcin Filip Osuchowski |
| author_facet |
Susanne Drechsler Katrin Weixelbaumer Pierre Raeven Mohammad Jafarmadar Anna Khadem Martijn van Griensven Soheyl Bahrami Marcin Filip Osuchowski |
| author_sort |
Susanne Drechsler |
| title |
Relationship between age/gender-induced survival changes and the magnitude of inflammatory activation and organ dysfunction in post-traumatic sepsis. |
| title_short |
Relationship between age/gender-induced survival changes and the magnitude of inflammatory activation and organ dysfunction in post-traumatic sepsis. |
| title_full |
Relationship between age/gender-induced survival changes and the magnitude of inflammatory activation and organ dysfunction in post-traumatic sepsis. |
| title_fullStr |
Relationship between age/gender-induced survival changes and the magnitude of inflammatory activation and organ dysfunction in post-traumatic sepsis. |
| title_full_unstemmed |
Relationship between age/gender-induced survival changes and the magnitude of inflammatory activation and organ dysfunction in post-traumatic sepsis. |
| title_sort |
relationship between age/gender-induced survival changes and the magnitude of inflammatory activation and organ dysfunction in post-traumatic sepsis. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/a4e7a65599224057bdaa71109c76b11f |
| work_keys_str_mv |
AT susannedrechsler relationshipbetweenagegenderinducedsurvivalchangesandthemagnitudeofinflammatoryactivationandorgandysfunctioninposttraumaticsepsis AT katrinweixelbaumer relationshipbetweenagegenderinducedsurvivalchangesandthemagnitudeofinflammatoryactivationandorgandysfunctioninposttraumaticsepsis AT pierreraeven relationshipbetweenagegenderinducedsurvivalchangesandthemagnitudeofinflammatoryactivationandorgandysfunctioninposttraumaticsepsis AT mohammadjafarmadar relationshipbetweenagegenderinducedsurvivalchangesandthemagnitudeofinflammatoryactivationandorgandysfunctioninposttraumaticsepsis AT annakhadem relationshipbetweenagegenderinducedsurvivalchangesandthemagnitudeofinflammatoryactivationandorgandysfunctioninposttraumaticsepsis AT martijnvangriensven relationshipbetweenagegenderinducedsurvivalchangesandthemagnitudeofinflammatoryactivationandorgandysfunctioninposttraumaticsepsis AT soheylbahrami relationshipbetweenagegenderinducedsurvivalchangesandthemagnitudeofinflammatoryactivationandorgandysfunctioninposttraumaticsepsis AT marcinfiliposuchowski relationshipbetweenagegenderinducedsurvivalchangesandthemagnitudeofinflammatoryactivationandorgandysfunctioninposttraumaticsepsis |
| _version_ |
1718422247670022144 |